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Method for preparing nano silver through pyrenochaeta penicillium

A technology of Penicillium aculeatus and Penicillium aculeatus, which is applied in the direction of nanotechnology, metal processing equipment, transportation and packaging, etc. It can solve the problems affecting the application of nanomaterials, harsh conditions of existence, high price, etc., and achieve safe reaction conditions Controllable, unique biological properties, good bio-affinity effects

Inactive Publication Date: 2017-08-18
HUNAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of preparing nano-silver by chemical method is that it is easy to operate, flexible and changeable, and the size of nano-particles is small, but it needs to use expensive and highly toxic reducing agent, stabilizer or dispersant, which is easy to cause harm to the environment. By-products affect the application of nanomaterials, especially in the medical field
Physical methods mainly include mechanical grinding method, vacuum condensation deposition method and laser turbidity method. The advantages are that the reaction principle is simple, and the obtained product has less impurities and high purity, but requires high equipment requirements, high energy consumption, and low yield. There are harsh conditions, high reaction energy consumption, high cost, and a single reaction product. Therefore, there is an urgent need for a green, environmentally friendly, mild reaction condition, low energy consumption, and low-cost nano-silver preparation method

Method used

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  • Method for preparing nano silver through pyrenochaeta penicillium
  • Method for preparing nano silver through pyrenochaeta penicillium
  • Method for preparing nano silver through pyrenochaeta penicillium

Examples

Experimental program
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Effect test

Embodiment 1

[0038] A kind of method utilizing Penicillium aculeatus to prepare nano-silver

[0039] (1) Obtaining Penicillium aculeatus strains: Inoculate a strain of Penicillium aculeatus on a PDA plate medium, culture it upside down at 30 °C for 72 h to activate the strain, and then add 3 mL of sterile water to the above PDA plate , to obtain a single spore suspension, the concentration of the single spore suspension is 8 × 10 6 spores / mL, take 200 μL of the single spore suspension and add it to 250 mL of sterilized PDB liquid medium, and shake it at 30°C with a rotation speed of 160 rpm for 72 hours. Collect the Penicillium aculeatus thallus by net filtration, and repeatedly wash with sterile deionized water until there is no medium component on the Penicillium aculeatus thallus;

[0040] (2) Preparation of Penicillium aculeatus cell soaking solution: Mix the Penicillium aculeatus cell obtained in step (1) with sterile deionized water at a mass volume ratio of 20 g: 100 mL, then incu...

Embodiment 2

[0043] Effect of the mass-volume ratio of Penicillium aculeatus to water on the preparation of nano-silver during secondary fermentation

[0044] Step (1) is as embodiment 1;

[0045] (2) Preparation of Penicillium aculeatus cell soaking solution: the Penicillium aculeatus cell obtained in step (1) and sterile deionized water were respectively mass-volume ratio 5g: 100 mL, 10g: 100 mL, 15g: 100 mL , 20g: 100 mL, 30g: 100 mL were mixed and cultured for 48 hours at 30°C with a rotation speed of 160 rpm. The five groups of fermentation broth were centrifuged at a rotation speed of 12,000 rpm for 5 minutes, and collected by Whatman No.1 filter paper. clear liquid to prepare 5 groups of Penicillium aculeatus thalline soaking liquids;

[0046] (3) Add silver ions to the five groups of Penicillium aculeatus cell soaking solutions prepared in step (2) respectively, so that the concentration of silver ions is 1.0 mmol / L, adjust the pH value to 8, and react in the dark at 30°C for 72 h...

Embodiment 3

[0048] Effect of fermentation time on the preparation of nano-silver in the secondary fermentation process

[0049] Step (1) is as embodiment 1;

[0050] (2) Preparation of Penicillium aculeatus cell soaking solution: Mix the Penicillium aculeatus cell obtained in step (1) with sterile deionized water at a mass volume ratio of 10 g: 100 mL, and then incubate at 30°C with a rotational speed of 160 rpm Cultivate under shaking for 24 h, 48 h, and 72 h respectively, centrifuge the three groups of fermentation liquids at 12000 rpm for 5 min, filter and collect the supernatant with Whatman No.1 filter paper, and prepare three groups of Penicillium aculeatus cells soaked liquid;

[0051] Step (3) is as in Example 2. During the preparation process, the color change of the reaction solution was observed, and the full wavelength scanning of the reaction solution was carried out with a UV-visible spectrophotometer. The detection range was 300-700 nm, and the scanning interval was 0.5 n...

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Abstract

The invention relates to a method for preparing nano silver through pyrenochaeta penicillium and belongs to the field of nano material preparation. According to the method, a pyrenochaeta penicillium chrysogenum soak solution mainly serves as a reaction solution to prepare the nano silver, operation is easy, no other reducing agent or additive needs to be added, the whole process is environmentally friendly, safe and non-toxic, reaction conditions are safe and controllable, the nano silver prepared through the method is spherical or approximately spherical, and the particle size is 5-60 nm. Due to the fact that reductase and other bioactivators in the pyrenochaeta penicillium chrysogenum soak solution participate in the process that silver nitrate is reduced into the nano silver and also wrap the surfaces of silver nano particles after the silver nano particles are formed, protein coats are formed, compared with nano silver and silver nitrate which are synthesized through a chemical method, the nano silver has unique biological characteristics and good biocompatibility, the nano silver can enter microbial cells more easily and disturb normal metabolism of microorganisms so that the microorganisms can be dead, thus, better bacteriostatic activity is achieved, application of the nano silver in the field of biomedicine is more advantageous, and the application prospects are broader.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation, and in particular relates to a method for preparing nanosilver by using Penicillium aculeatus. Background technique [0002] In recent years, with the continuous development of nano-silver preparation technology, the application of nano-silver has become more and more extensive. Silver nanomaterials have special physical and chemical properties, and are widely used in important fields such as industry and medicine. In industrial production, nano-silver has better catalytic activity than silver, and the addition of nano-silver can effectively improve the optical, thermal, magnetic and other characteristics of the product. In the field of medicine, nano-silver is often used as a drug for the prevention and treatment of diseases. At present, the synthesis methods of nano-silver mainly include chemical methods and physical methods. Among them, the development of chemical methods is...

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/02B82Y40/00
CPCB82Y40/00B22F9/24B22F1/054B22F1/065B22F1/16
Inventor 马靓汤建新刘纯
Owner HUNAN UNIV OF TECH
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