Airlift type loop reactor and method for penicillin fermentation by using the same

Abstract

The present invention discloses an airlift type loop reactor and a method for penicillin fermentation by using the airlift type loop reactor. The lower part of the tower body of the airlift type loop reactor is the reaction section, and the upper part is the expanded gas-liquid separation section, wherein a height ratio of the gas-liquid separation section to the reaction section is 0.15-0.25, a diameter ratio is 1.25-1.42, a ratio of the total height of the tower body to the inner diameter of the reaction section is 4.5-8, a ratio of the inner diameter of a draft tube to the inner diameter of the tower body is 0.5-0.78, and a height ratio of the draft tube to the reaction section is 0.9-0.65. In the airlift type loop reactor, air is introduced, and a strain producing penicillium chrysogenum is adopted to produce penicillin, wherein the fermentation temperature is 20-30 DEG C, the pH value is 6.0-7.0, the fermentation pressure is 0.08-0.1 MPa, the ventilation ratio is controlled to 0.8-3 vvm, and the fermentation time is 160-170 hours.

Description

technical field [0001] The invention relates to a reactor for fermenting penicillin and a method for fermenting penicillin using the reactor, in particular to an airlift loop reactor and a method for penicillin fermentation using the airlift loop reactor. Background technique [0002] The aerobic fermentation of the filamentous bacterial system with high viscosity non-Newtonian fluid, such as penicillin fermentation, generally adopts a tank-type fermentation reactor with mechanical agitation, and the production is carried out under the condition of introducing air, and the mechanical agitation makes the air evenly distributed in the In the tank, to ensure the growth and metabolism of the bacteria, but there are two important problems in this method: on the one hand, the energy consumption of the stirring motor and the energy consumption of the compressed air make the energy consumption of the fermentation process generally high. Power consumption accounts for 30-50% of the c...

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Problems solved by technology

[0002] The aerobic fermentation of the filamentous bacterial system with high viscosity non-Newtonian fluid, such as penicillin fermentation, generally adopts a tank-type fermentation reactor with mechanical agitation, and the production is carried out under the condition of introducing air, and the mechanical agitation makes the air evenly distributed in the In the tank, to ensure the growth and metabolism of the bacteria, but there are two important problems in this method: on the one hand, the energy consumption of the stirring motor and the energy consumption of the compressed air make the energy consumption of the fermentation process generally high. Power consumption accounts for 30-50% of the cost. In the production of penicillin fermentation, a mechanically stirred fermenter is used for fermentation, and the average power consumption is 20-25Kwh / BOU (BOU penicillin activity unit, the same below); on the other hand, mechanical The shear force of stirring also affects the growth of mycelium

[0006] (1) Because the circulation driving force of the airlift loop reactor (the density difference between gas and liquid inside and outside the draft tube) is small, it often fails to meet the needs of dissolved oxygen in the fermentation process; or requires an excessive gas velocity to maintain Circulation, making the process less economical and making the reactor difficult to operate

[0007] (2) The fluid shear force of the airlift loop reactor is small. In the primary metabolic stage of Penicillium, in the case of sufficient gas and nutrient supply, if the external shear force is small, Penicillium is easy to form agglomeration , is not conducive to the production of resistance in the secondary metabolic stage

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