3results about How to "Raise the fermentation unit" patented technology

The invention provides a method for increasing the fermentation unit of acarbose. The method mainly comprises the step of adding metal ions into a fermentation culture medium. According to the method, metal ions such as copper sulfate, zinc sulfate or manganese sulfate and the like are added into the fermentation culture medium, and ferric chloride is added in a matched manner, so that the fermentation titer of acarbose is increased to 8.83 mu g / mL under the condition of not changing strains and the fermentation culture medium, and the fermentation unit of acarbose is remarkably increased; in addition, the fermentation period is shortened, and the production cost is reduced.

The present invention relates to a culture medium and a fermentation method for improving the fermentation unit of tylosin, which is a culture medium for producing tylosin by fermenting Streptomyces freundii, comprising a primary seed culture medium, a secondary seed culture medium and a fermentation culture medium, wherein the primary seed culture medium, the secondary seed culture medium and the fermentation culture medium all contain palm oil and petroleum yeast powder, and the fermentation culture medium contains oxygen carriers and n-dodecane. The method adopts a new formula, which can greatly shorten the fermentation cycle by about 18 hours compared with the traditional process, reduce energy consumption and save the production cost of raw materials and auxiliary materials; the use of palm oil and corn starch milk can reduce the total cost by about 25%; the use of petroleum yeast powder and oxygen carriers and n-dodecane in a reasonable ratio in a tylosin fermentation tank can accelerate the production speed on the basis of increasing the palm oil consumption rate, so that the fermentation unit is greatly increased.

The application discloses a culture method for improving the fermentation yield of tacrolimus and belongs to the technical field of tacrolimus production. The culture method for improving the fermentation yield of tacrolimus comprises the following steps: inoculating Streptomyces tsukuba into a fermentation culture medium for fermentation culture to obtain a tacrolimus fermentation liquor; and performing separation and purification on the tacrolimus fermentation liquor to obtain tacrolimus; and adding macroporous adsorption resin into the fermentation culture medium according to the fermentation time during the fermentation process. The method for improving the fermentation yield of tacrolimus controls the carbon and nitrogen source concentration and pH in the fermentation process and supplements macroporous resin, improves the secondary metabolism efficiency, adsorbs tacrolimus in the fermentation liquor through the macroporous adsorption resin, reduces the negative feedback inhibition effect on the secondary metabolism of the production strain caused by high-concentration tacrolimus, and significantly improves the tacrolimus yield.