1results about How to "Raise the fermentation unit" patented technology

The application discloses a culture method for improving the fermentation yield of tacrolimus and belongs to the technical field of tacrolimus production. The culture method for improving the fermentation yield of tacrolimus comprises the following steps: inoculating Streptomyces tsukuba into a fermentation culture medium for fermentation culture to obtain a tacrolimus fermentation liquor; and performing separation and purification on the tacrolimus fermentation liquor to obtain tacrolimus; and adding macroporous adsorption resin into the fermentation culture medium according to the fermentation time during the fermentation process. The method for improving the fermentation yield of tacrolimus controls the carbon and nitrogen source concentration and pH in the fermentation process and supplements macroporous resin, improves the secondary metabolism efficiency, adsorbs tacrolimus in the fermentation liquor through the macroporous adsorption resin, reduces the negative feedback inhibition effect on the secondary metabolism of the production strain caused by high-concentration tacrolimus, and significantly improves the tacrolimus yield.