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Preparation method of monensin premix

A technology of monensin and premix, which is applied to the preparation of antibiotic premix and the field of preparation of monensin premix, can solve the problems of high production cost and environmental protection cost, unrealizable production expansion, and unfavorable morbidity. It can reduce the production cost, prevent pollution and avoid harm

Active Publication Date: 2015-01-28
金河生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the further development of animal husbandry, the market demand for Moneng premix has been expanding in recent years, and the quality requirements of domestic and foreign customers for Moneng premix are getting higher and higher, and some existing preparation methods have the following Disadvantages: the extraction process is complicated, the consumption of chemical solvents is large, resulting in a large amount of waste solvents and waste residues, and the production cost and environmental protection cost are relatively high; the product particle rate is low, which is not conducive to the dispersion of monensin in the feed; the fermentation process Raw materials that are strictly prohibited in domestic and foreign feed industries, such as fishmeal, are used in the feed, which makes it impossible to expand production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation method of monensin premix is ​​as follows:

[0028] a. Seed cultivation

[0029] 1) Strain preparation

[0030] Take the sand tube containing Streptomyces cinnamon, add 5 mL of sterile water under aseptic conditions and shake well, then apply it to the spore-forming slant medium with a sterile inoculation needle, and culture it at 32°C±0.5°C for 11 sky.

[0031] 2) Seed shake flask culture

[0032] Inoculate the spores prepared in step 1) into the culture medium of the shake flask, and cultivate at a constant temperature of 32±0.5° C. for 25 hours at a rotation speed of 220-260 rpm.

[0033] The culture medium of the shake flask culture is composed of the following components according to the mass percentage concentration: 1.5% of soybean cake powder, 0.2% of yeast powder, 0.4% of glucose, 1.0% of dextrin, 0.06% of calcium carbonate, and the balance is water; The pH value of the medium was 7.05.

[0034] 3) Seed tank culture

[0035] Add the strain...

Embodiment 2

[0045] The preparation method of monensin premix is ​​as follows:

[0046] a. Seed cultivation

[0047] 1) The strain preparation is the same as in Example 1.

[0048] 2) Seed shake flask culture

[0049] Operation is with embodiment 1.

[0050] The medium for shaking flask culture is composed of the following components in terms of mass percentage concentration: 1.4% of soybean cake powder, 0.2% of yeast powder, 0.35% of glucose, 1.5% of dextrin, 0.08% of calcium carbonate, and the balance is water. The pH of the medium after sterilization was 7.0.

[0051] 3) Seed tank culture

[0052] Add the strains cultivated in step 2) into the seed tank with an inoculation amount of 0.0035% seed solution, cultivate for 23 hours at a temperature of 32±0.5°C and a tank pressure of 0.04 MPa, and take samples for testing.

[0053] Test results: the appearance is light yellow, thick, normal smell, no bacteria, pH value is 6.9, biomass is 20%, monensin titer is 178U / mL, mycelium is stron...

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PUM

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Abstract

The invention discloses a preparation method of a monensin premix. The preparation method comprises the following specific steps: preparing cinnamon streptomyce strain; seed flask culture and seed tank culture are performed; when the age of the seed is 20-24h, the pH of a culture solution is 6.6-7.1, the biomass is above 10%, and microscopic examination shows that mycelia are thick and reticular, are stretched and darkly stained and are free of foreign bacteria, performing fermentation culture is performed; when the residual oil amount is below 0.7%, the biomass is above 40%, the mycelium concentration is 40-50%, and the monensin titer is not lower than 40000U / mL, fermentation is finished; monensin is extracted by a one-step spray drying method, wherein the prepared finished monensin product has the monensin content of above 20% and the grain rate of above 75%. According to the preparation method, the fermentation process is simple, so that the fermentation unit can be effectively increased and the production cost can be reduced; usages of an animal-derived nitrogen source and a chemical organic solvent are avoided.

Description

technical field [0001] The invention belongs to the field of veterinary medicine, and relates to a preparation method of an antibiotic premix, in particular to a preparation method of a monensin premix. Background technique [0002] Monensin, a polyether antibiotic, was first isolated from the fermentation broth of Streptomyces cinnamonensis by Haney et al. in 1967. In the 1970s, it was put on the market as an anticoccidial drug. The United States and Japan officially approved it as a feed additive in 1974 and 1977 respectively, and my country was approved in 1985 and applied to production. At present, more than 40 countries have used it as a weight gain agent for beef cattle and mutton sheep and a growth promoter for pigs. Monensin has no effect on negative bacteria, but has a certain inhibitory effect on Staphylococcus, Bacillus, Clostridium, Streptococcus, and mold (Penicillium, Candida). Monensin can affect the energy metabolism in the rumen of ruminants, improve rume...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/16C12R1/465
Inventor 谢昌贤邓维康刘运添王鹏飞
Owner 金河生物科技股份有限公司
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