Preparation method of monensin premix
A technology of monensin and premix, which is applied to the preparation of antibiotic premix and the field of preparation of monensin premix, can solve the problems of high production cost and environmental protection cost, unrealizable production expansion, and unfavorable morbidity. It can reduce the production cost, prevent pollution and avoid harm
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Embodiment 1
[0027] The preparation method of monensin premix is as follows:
[0028] a. Seed cultivation
[0029] 1) Strain preparation
[0030] Take the sand tube containing Streptomyces cinnamon, add 5 mL of sterile water under aseptic conditions and shake well, then apply it to the spore-forming slant medium with a sterile inoculation needle, and culture it at 32°C±0.5°C for 11 sky.
[0031] 2) Seed shake flask culture
[0032] Inoculate the spores prepared in step 1) into the culture medium of the shake flask, and cultivate at a constant temperature of 32±0.5° C. for 25 hours at a rotation speed of 220-260 rpm.
[0033] The culture medium of the shake flask culture is composed of the following components according to the mass percentage concentration: 1.5% of soybean cake powder, 0.2% of yeast powder, 0.4% of glucose, 1.0% of dextrin, 0.06% of calcium carbonate, and the balance is water; The pH value of the medium was 7.05.
[0034] 3) Seed tank culture
[0035] Add the strain...
Embodiment 2
[0045] The preparation method of monensin premix is as follows:
[0046] a. Seed cultivation
[0047] 1) The strain preparation is the same as in Example 1.
[0048] 2) Seed shake flask culture
[0049] Operation is with embodiment 1.
[0050] The medium for shaking flask culture is composed of the following components in terms of mass percentage concentration: 1.4% of soybean cake powder, 0.2% of yeast powder, 0.35% of glucose, 1.5% of dextrin, 0.08% of calcium carbonate, and the balance is water. The pH of the medium after sterilization was 7.0.
[0051] 3) Seed tank culture
[0052] Add the strains cultivated in step 2) into the seed tank with an inoculation amount of 0.0035% seed solution, cultivate for 23 hours at a temperature of 32±0.5°C and a tank pressure of 0.04 MPa, and take samples for testing.
[0053] Test results: the appearance is light yellow, thick, normal smell, no bacteria, pH value is 6.9, biomass is 20%, monensin titer is 178U / mL, mycelium is stron...
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