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Method for preparing apramycin through fermentation

A technology for the production of apramycin and strains, which is applied in the field of microbial fermentation and can solve problems such as not achieving good results

Inactive Publication Date: 2014-12-24
HEBEI SHENGXUE DACHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Apramycin is produced by the fermentation of Streptomyces darker. At present, the research on the production of apramycin by microbial fermentation is mainly the mutagenesis treatment of strains, the optimization of fermentation medium, or the optimization of fermentation process, and no good results have been achieved. Effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The composition of the slant medium by mass percentage is: 2.3% corn starch, 0.2% potassium nitrate, 0.05% magnesium sulfate, 0.05% sodium chloride, 0.05% potassium dihydrogen phosphate, 0.2% yeast powder, 0.3% calcium carbonate, 2.5% agar %.

[0022] The composition of seed bottle culture medium by mass percentage is: glucose 2.0%, cornstarch 2.0%, low-temperature soybean cake powder 2.5%, calcium carbonate 0.3%, magnesium sulfate 0.1%, yeast powder 0.2%, ammonium sulfate 0.15%, corn steep liquor 2% %.

[0023] The composition of the fermentation medium according to mass percentage is: 4.0% of low-temperature soybean meal powder, 6.0% of corn starch, 0.5% of calcium carbonate, 0.2% of potassium dihydrogen phosphate, 0.6% of ammonium chloride, 0.03% of zinc sulfate, 0.1% of magnesium sulfate, Amylase 0.15%.

[0024] a. Slope culture: Inoculate the sand spores of the production strain of Streptomyces obscurus into the slope culture medium, the inoculum amount of the sa...

Embodiment 2

[0030] The composition of the slant medium by mass percentage is: 2.0% corn starch, 0.1% potassium nitrate, 0.025% magnesium sulfate, 0.04% sodium chloride, 0.04% potassium dihydrogen phosphate, 0.1% yeast powder, 0.1% calcium carbonate, 2.0% agar %.

[0031] The composition of the seed bottle medium by mass percentage is: 2.0% of glucose, 1.5% of corn starch, 2.0% of low-temperature soybean cake powder, 0.2% of calcium carbonate, 0.05% of magnesium sulfate, 0.1% of yeast powder, 0.1% of ammonium sulfate, and 1% of corn steep liquor. %.

[0032] The composition of the fermentation medium according to mass percentage is: 3.0% of low-temperature soybean meal powder, 5.0% of corn starch, 0.4% of calcium carbonate, 0.1% of potassium dihydrogen phosphate, 0.6% of ammonium chloride, 0.02% of zinc sulfate, 0.05% of magnesium sulfate, Amylase 0.1%.

[0033] a. Slope culture: inoculate the sand spores of the production strain of Streptomyces obscura into the slope culture medium, the...

Embodiment 3

[0039] The composition of the slant medium by mass percentage is: 2.5% corn starch, 0.2% potassium nitrate, 0.05% magnesium sulfate, 0.06% sodium chloride, 0.06% potassium dihydrogen phosphate, 0.2% yeast powder, 0.3% calcium carbonate, 2.5% agar %.

[0040]The composition of the seed bottle medium according to mass percentage is: 2.5% glucose, 2.0% corn starch, 3.0% low-temperature soybean cake powder, 0.4% calcium carbonate, 0.1% magnesium sulfate, 0.3% yeast powder, 0.15% ammonium sulfate, and 2% corn steep liquor. %.

[0041] The composition of the fermentation medium according to mass percentage is: 4.0% of low-temperature soybean meal powder, 7.0% of corn starch, 0.5% of calcium carbonate, 0.2% of potassium dihydrogen phosphate, 0.7% of ammonium chloride, 0.03% of zinc sulfate, 0.1% of magnesium sulfate, Amylase 0.15%.

[0042] a. Slope culture: Inoculate the sand spores of the production strain of Streptomyces obscura into the slant medium, the inoculation amount of t...

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Abstract

The invention discloses a method for preparing apramycin through fermentation. The method comprises the following steps: performing slant culture, culturing a seed liquid, and performing fermentation culture. The method is simple to operate, and due to replenishment of corn steep liquor in the fermentation culture, the fermentation unit of apramycin is greatly increased. The fermentation unit in apramycin prepared by the method can be up to 8400U / mL.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for fermenting and producing apramycin. Background technique [0002] Apramycin is an aminoglycoside antibiotic, more specifically, its chemical structure contains a suberic diose structure. Apramycin has the characteristics of broad antibacterial spectrum, strong effect, less residue, low toxicity, and is not easy to produce drug resistance. It is widely used in infections caused by Escherichia coli, Salmonella and mycoplasma. As a drug-type feed additive, apramycin can significantly promote weight gain and improve feed conversion rate, and has been widely used in the field of livestock and poultry breeding. [0003] Apramycin is produced by the fermentation of Streptomyces darker. At present, the research on the production of apramycin by microbial fermentation is mainly the mutagenesis treatment of strains, the optimization of fermentation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/48C12R1/465
Inventor 李可乐刘聪洁袁昉王绘砖李敏
Owner HEBEI SHENGXUE DACHENG PHARMA
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