Method for preparing apramycin through fermentation
A technology for the production of apramycin and strains, which is applied in the field of microbial fermentation and can solve problems such as not achieving good results
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Embodiment 1
[0021] The composition of the slant medium by mass percentage is: 2.3% corn starch, 0.2% potassium nitrate, 0.05% magnesium sulfate, 0.05% sodium chloride, 0.05% potassium dihydrogen phosphate, 0.2% yeast powder, 0.3% calcium carbonate, 2.5% agar %.
[0022] The composition of seed bottle culture medium by mass percentage is: glucose 2.0%, cornstarch 2.0%, low-temperature soybean cake powder 2.5%, calcium carbonate 0.3%, magnesium sulfate 0.1%, yeast powder 0.2%, ammonium sulfate 0.15%, corn steep liquor 2% %.
[0023] The composition of the fermentation medium according to mass percentage is: 4.0% of low-temperature soybean meal powder, 6.0% of corn starch, 0.5% of calcium carbonate, 0.2% of potassium dihydrogen phosphate, 0.6% of ammonium chloride, 0.03% of zinc sulfate, 0.1% of magnesium sulfate, Amylase 0.15%.
[0024] a. Slope culture: Inoculate the sand spores of the production strain of Streptomyces obscurus into the slope culture medium, the inoculum amount of the sa...
Embodiment 2
[0030] The composition of the slant medium by mass percentage is: 2.0% corn starch, 0.1% potassium nitrate, 0.025% magnesium sulfate, 0.04% sodium chloride, 0.04% potassium dihydrogen phosphate, 0.1% yeast powder, 0.1% calcium carbonate, 2.0% agar %.
[0031] The composition of the seed bottle medium by mass percentage is: 2.0% of glucose, 1.5% of corn starch, 2.0% of low-temperature soybean cake powder, 0.2% of calcium carbonate, 0.05% of magnesium sulfate, 0.1% of yeast powder, 0.1% of ammonium sulfate, and 1% of corn steep liquor. %.
[0032] The composition of the fermentation medium according to mass percentage is: 3.0% of low-temperature soybean meal powder, 5.0% of corn starch, 0.4% of calcium carbonate, 0.1% of potassium dihydrogen phosphate, 0.6% of ammonium chloride, 0.02% of zinc sulfate, 0.05% of magnesium sulfate, Amylase 0.1%.
[0033] a. Slope culture: inoculate the sand spores of the production strain of Streptomyces obscura into the slope culture medium, the...
Embodiment 3
[0039] The composition of the slant medium by mass percentage is: 2.5% corn starch, 0.2% potassium nitrate, 0.05% magnesium sulfate, 0.06% sodium chloride, 0.06% potassium dihydrogen phosphate, 0.2% yeast powder, 0.3% calcium carbonate, 2.5% agar %.
[0040]The composition of the seed bottle medium according to mass percentage is: 2.5% glucose, 2.0% corn starch, 3.0% low-temperature soybean cake powder, 0.4% calcium carbonate, 0.1% magnesium sulfate, 0.3% yeast powder, 0.15% ammonium sulfate, and 2% corn steep liquor. %.
[0041] The composition of the fermentation medium according to mass percentage is: 4.0% of low-temperature soybean meal powder, 7.0% of corn starch, 0.5% of calcium carbonate, 0.2% of potassium dihydrogen phosphate, 0.7% of ammonium chloride, 0.03% of zinc sulfate, 0.1% of magnesium sulfate, Amylase 0.15%.
[0042] a. Slope culture: Inoculate the sand spores of the production strain of Streptomyces obscura into the slant medium, the inoculation amount of t...
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