Fermentation medium for fermentation production of antibiotic calicheamicin gamma1 I, fermentation method suitable for the fermentation medium and use of the fermentation medium
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A technology of fermentation medium and antibiotics, which is applied in the field of industrial microorganisms and can solve the problems of low yield and low fermentation units
Inactive Publication Date: 2012-10-24
SHANGHAI INST OF PHARMA IND
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Problems solved by technology
[0005] The technical problem to be solved by the present invention is exactly to utilize Micromonospora aculeatus fermentation to produce antibiotic calicheamicin gamma 1 The fermentation medium fermentation unit of I is low, and the defect such as low yield provides a new method for Micromonospora aculeatus fermentation to produce antibiotic calicheamicinγ 1 The fermentation medium of I and corresponding fermentation method and purposes, thereby improve calicheamicin gamma 1 The fermentation unit of I improves production efficiency and reduces production costs
Method used
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[0048] The seed liquid that makes according to 1 in comparative example is inoculated with 10% (v / v) inoculum amount and is fermented in the 250ml fermenting shaker flask that liquid filling volume is 20ml, and fermentation temperature is 30 ℃, and fermentation period is 6d, and other conditions are the same as contrast Example 1. calicheamicin| 1 I yield was 119%.
Embodiment 2
[0050] Fermentation medium composition: sucrose 18.0g / L, molasses 7.0g / L, fine bean cypress 4.0g / L, FeSO 4 ·7H 2 O 0.1g / L, MgSO 4 ·7H 2 O 0.2g / L, KI 0.1g / L, CaCO 3 2.5g / L, pH7.2.
[0051] The seed liquid that makes according to 1 in comparative example is inoculated with 10% (v / v) inoculum amount and is fermented in the 250ml fermenting shaker flask that liquid filling volume is 20ml, and fermentation temperature is 30 ℃, and fermentation period is 6d, and other conditions are the same as contrast Example 1. calicheamicin| 1 I yield was 121%.
Embodiment 3
[0053] Composition of fermentation medium: 22.0g / L sucrose, 8.0g / L molasses, 4.0g / L corn steep liquor powder, 4.0g / L fine soybean meal, 4.0g / L cold-pressed soybean cake powder, 4.0g / L tryptone, FeSO 4 ·7H 2O 0.1g / L, MgSO 4 ·7H 2 O 0.2g / L, KI 0.1g / L, CaCO 3 2.5g / L, pH7.2.
[0054] The seed solution prepared by 1 in Comparative Example is inoculated with a 10% (v / v) inoculum amount in a 250ml fermentation shaker flask with a liquid filling volume of 20ml for fermentation, the fermentation temperature is 30°C, and the fermentation period is 6d. Other conditions are the same as compared Example 1. calicheamicin| 1 I yield was 125%.
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Abstract
The invention provides a fermentation medium for fermentation production of an antibiotic calicheamicin gamma1 I. The fermentation medium comprises one or more organic carbon sources and one or more organic nitrogen sources, wherein based on a total volume of the fermentation medium, the content of the one or more organic carbon sources is in a range of 5 to 130g/L and is preferably in a range of 20 to 110g/L; the content of the one or more organic nitrogen sources is in a range of 2 to 150g/L, is preferably in a range of 4 to 60g/L and is more preferably in a range of 15 to 40g/L; the one or more organic carbon sources are selected from cane sugar, molasses, glycerin and corn starch; and the one or more organic nitrogen sources are selected from fine bean meal and corn steep powder. The invention also provides a fermentation method for fermentation production of an antibiotic calicheamicin gamma1 I by the fermentation medium and a use of the fermentation medium. The fermentation medium greatly provides fermentation titer units of calicheamicin gamma1 I, improves production efficiency, reduces a production cost, and is suitable for large-scale production of calicheamicin gamma1 I.
Description
technical field [0001] The invention belongs to the technical field of industrial microbes, in particular to an antibiotic calicheamicin gamma fermented by Micromonas aculeatus 1 The new fermented medium of I, also relate to produce antibiotic calicheamicin gamma by fermenting with this fermented medium 1 The method of 1 and the purposes of this fermentation medium. Background technique [0002] In 1987, the Lederle laboratory of the American cyanamide company (CYANAMID) isolated Micromonospora echinosporasp. After fermentation in the base, 7 different active compounds were obtained, mainly including: Calicheamicin β 1 Br, γ 1 Br, α 2 I, α 3 I, β 1 I, γ 1 I and δ 1 I. This anti-tumor antibiotic belongs to enediyne antibiotics, which can cause irreversible damage to cells by breaking cell DNA, thereby killing cancer cells. of which Calicheamicin 1 I has the highest anticancer activity. [0003] Due to the high toxicity of enediyne antibiotics, they have not been va...
Claims
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