Fermentation medium for fermentation production of antibiotic calicheamicin gamma1 I, fermentation method suitable for the fermentation medium and use of the fermentation medium

Abstract

The invention provides a fermentation medium for fermentation production of an antibiotic calicheamicin gamma1 I. The fermentation medium comprises one or more organic carbon sources and one or more organic nitrogen sources, wherein based on a total volume of the fermentation medium, the content of the one or more organic carbon sources is in a range of 5 to 130g / L and is preferably in a range of 20 to 110g / L; the content of the one or more organic nitrogen sources is in a range of 2 to 150g / L, is preferably in a range of 4 to 60g / L and is more preferably in a range of 15 to 40g / L; the one or more organic carbon sources are selected from cane sugar, molasses, glycerin and corn starch; and the one or more organic nitrogen sources are selected from fine bean meal and corn steep powder. The invention also provides a fermentation method for fermentation production of an antibiotic calicheamicin gamma1 I by the fermentation medium and a use of the fermentation medium. The fermentation medium greatly provides fermentation titer units of calicheamicin gamma1 I, improves production efficiency, reduces a production cost, and is suitable for large-scale production of calicheamicin gamma1 I.

Description

technical field [0001] The invention belongs to the technical field of industrial microbes, in particular to an antibiotic calicheamicin gamma fermented by Micromonas aculeatus 1 The new fermented medium of I, also relate to produce antibiotic calicheamicin gamma by fermenting with this fermented medium 1 The method of 1 and the purposes of this fermentation medium. Background technique [0002] In 1987, the Lederle laboratory of the American cyanamide company (CYANAMID) isolated Micromonospora echinosporasp. After fermentation in the base, 7 different active compounds were obtained, mainly including: Calicheamicin β 1 Br, γ 1 Br, α 2 I, α 3 I, β 1 I, γ 1 I and δ 1 I. This anti-tumor antibiotic belongs to enediyne antibiotics, which can cause irreversible damage to cells by breaking cell DNA, thereby killing cancer cells. of which Calicheamicin 1 I has the highest anticancer activity. [0003] Due to the high toxicity of enediyne antibiotics, they have not been va...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is exactly to utilize Micromonospora aculeatus fermentation to produce antibiotic calicheamicin gamma 1 The fermentation medium fermentation unit of I is low, and the defect such as low yield provides a new method for Micromonospora aculeatus fermentation to produce antibiotic calicheamicinγ 1 The fermentation medium of I and corresponding fermentation method and purposes, thereby improve calicheamicin gamma 1 The fermentation unit of I improves production efficiency and reduces production costs