The invention relates to a molecular marker for enhancement and releasing of pseudosciaena crocea and a screening method of the molecular marker. Themolecular marker is characterized by comprising 3,4-dihydroxymandelic acid, p-hydroxypheny lacetothiohydroximate, 2-propylglutaric acid, cortisol, ergothioneine, (R)-mevalonate, cyclic dehypoxanthine futalosine, mevalonic acid, biotin, 8-amino-7-oxononanoate, N-arachidonyl dopamine, adrenosterone, delta1-piperideine-2-carboxylate, creatine, calicheamicin, T0 L-2-aminoadipate 6-semialdehyde and the like.The screening method comprises the following steps: taking pseudosciaena crocea muscle samples which are temporarily cultured different times, qualitatively and quantitatively determining small molecule metabolites of pseudosciaena croceamuscle tissues by adopting LC-MS/MS, determining the time when pseudosciaena crocea fries adapt to a releasing water area through volcano plots and principal component analysis, and screening through partial least square discriminant analysis and Wien diagram. The method has the advantages that the temporary rearing cost of the pseudosciaena crocea is reduced, and the enhancement and release survival rate is improved.