Industrial fermentation method of riboflavin

A riboflavin and industrial technology, applied in the field of riboflavin industrial fermentation, can solve the problems of unfavorable riboflavin production and instability, and achieve the effects of alleviating the problem of insufficient ventilation and oxygen supply, increasing the synthesis speed and shortening the fermentation cycle

Active Publication Date: 2011-08-17
GUANGJI PHARMA MENGZHOU
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Problems solved by technology

This feeding method is greatly affected by changes in the external system, causing the reducing sugar content in the fermentation broth to fluctuate and become unstable, which is not conducive to the production of riboflavin

Method used

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Embodiment

[0028] The method for riboflavin industrial fermentation, its steps are as follows:

[0029] (1) Fill the seed medium into 10 m 3 After the disinfection of the seed tank is completed, insert the prepared Bacillus subtilis spore suspension and cultivate it aerobically for 16-18 hours. The conditions of the seed medium are: biological nitrogen 15g / L, corn steep liquor 0.2g / L, glucose 5g / L, pH 6.8~7.0;

[0030] (2) Fill the fermentation medium into 200m 3 Fermentation tank, add 40kg of sterilized defoamer, the amount of defoamer accounts for 0.02%~0.05% of the total material mass, the conditions of the fermentation medium are: biological nitrogen 3g / L, corn steep liquor 1.2g / L, betaine 0.01g / L, pH6.8~7.2;

[0031] (3) Under the condition of introducing sterile clean air, add the sterilized starch glucose solution at one time to make the glucose concentration reach 2g / L. After disinfection, transfer the cultured seed solution into it and start fermentation, keeping the fermenta...

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PUM

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Abstract

The invention discloses an industrial fermentation method of riboflavin, which comprises the following steps of: filling a seed culture medium into a 10m<3> seed tank, and performing oxic cultivation; and filling a fermentation medium into a 200m<3> fermentation tank for fermentation, mechanically stirring, adding sterilization starch glucose liquid, stopping fermenting till that the glucose is completely consumed or the dissolved oxygen is raised to be 60%, and extracting the riboflavin from the fermentation liquid. After the industrial fermentation method is used, the conventional riboflavin material-supplementing fermentation technology is changed, the low-concentration raw material cultivating bacteria is adopted, the material is quantificationally supplemented according to the changeof the dissolved oxygen of the fermentation solution, the excessive multiplication of the thallus can be effectively controlled, the deviation of the optimum control temperature caused by the excessive supersession can be avoided, the defects of the insufficient ventilation and oxygen supply can be remitted, and the thallus vigor can be kept, so that the indusial fermentation unit of the riboflavin is improved by more than 18g/L, and the yield is increased by 20% compared with the common fermentation.

Description

technical field [0001] The invention relates to a method for riboflavin industrial fermentation. Background technique [0002] The microbial fermentation production of riboflavin adopts three-stage fermentation method. The slant spores of the mature riboflavin-producing bacteria are made into a spore suspension with sterile water, inoculated in the seed medium for cultivation, and finally the secondary fermentation liquid is transplanted to the third-stage fermentation tank for fermentation to obtain riboflavin fermentation liquid . Different riboflavin manufacturers use different ingredients. At present, the main carbon sources used in the microbial fermentation method are vegetable oil, glucose, molasses or rice sugar, etc.; the organic nitrogen sources are mainly peptone, bone glue, fish meal, and corn steep liquor; the inorganic salts include NaCl, K 2 HPO 4 , MgSO 4 Wait. The raw materials used lead to high costs in industrial applications, and the supply is diffi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P25/00
Inventor 何谧王专旭高峰胡发强
Owner GUANGJI PHARMA MENGZHOU
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