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Fermentation culture medium and fermentation method for improving enramycin yield

The technology of fermentation medium and enramycin, which is applied in the field of microbial fermentation, can solve the problems of high production cost of susceptible bacteria, enramycin, and unsatisfactory fermentation results, and achieves the effect of improving the fermentation unit.

Active Publication Date: 2014-11-05
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the production cost of enramycin is relatively high, the fermentation result is unsatisfactory, and it is easy to infect bacteria.

Method used

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  • Fermentation culture medium and fermentation method for improving enramycin yield
  • Fermentation culture medium and fermentation method for improving enramycin yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Inoculate the experimental bacteria on a solid slant medium under aseptic conditions, and culture at a constant temperature of 28°C for 7 days; cultivate the fungicide Streptomyces in the seed medium to obtain a seed liquid, and the seed liquid is 8%-10% (v / v) inoculum amount is inoculated in a 50L fermenter with a liquid content of 35L of fermentation medium for fermentation, the fermentation temperature is 28°C, and the fermentation period is 8 days. Samples were taken (HPLC) after fermentation to determine that the titer of enramycin could reach 9133 μg / mL.

[0028] Wherein, the composition of the solid slant medium is: glucose 10g / L, tryptone 1g / L, agar 22g / L, yeast powder 1g / L.

[0029] The composition of described seed culture medium is: corn meal 40g / L, corn steep liquor 28g / L, cottonseed meal meal 5g / L, corn gluten meal 5g / L, ammonium sulfate 3g / L, ferrous sulfate 0.36g / L, phosphoric acid Potassium dihydrogen 1.25g / L, calcium carbonate 22g / L, foam enemy 0.6g / L,...

Embodiment 2

[0032] The experimental strains were inoculated on a solid slant medium under sterile conditions, and cultured at a constant temperature of 28°C for 7 days; the seed liquid was inoculated in the fermentation medium with an inoculation amount of 8%-10%, the fermentation temperature was 28°C, and the fermentation period was 10 days . Samples were taken (HPLC) after fermentation to determine that the titer of enramycin could reach 8517 μg / mL.

[0033] Described solid slant culture medium and seed culture medium are with embodiment 1;

[0034] The composition of described fermentation medium: glucose 40g / L, cornstarch 15g / L, soybean meal powder 21g / L, cottonseed cake powder 10g / L, corn steep liquor hydrolyzate 25g / L, yeast extract powder 1g / L, NaCl15g / L , NH 4 Cl5g / L, CaCO 3 20g / L, foam enemy 0.6g / L, adjust the pH to 7.1 with 20% liquid alkali, and finally put in calcium carbonate.

Embodiment 3

[0036] The experimental strains were inoculated on a solid slant medium under sterile conditions, and cultured at a constant temperature of 28°C for 7 days; the seed liquid was inoculated in the fermentation medium with an inoculation amount of 8%-10%, the fermentation temperature was 28°C, and the fermentation period was 9 days . Sampling (HPLC) was performed after fermentation, and the titer of enramycin could reach 9048 μg / mL.

[0037] Described solid slant culture medium and seed culture medium are with embodiment 1;

[0038]The composition of described fermentation medium: glucose 40g / L, cornstarch 25g / L, soybean meal powder 21g / L, cottonseed cake powder 10g / L, corn steep liquor hydrolyzate 20g / L, yeast extract powder 1g / L, NaCl15g / L , NH 4 Cl5g / L, CaCO 3 20g / L, foam enemy 0.6g / L, adjust the pH to 7.0 with 20% liquid alkali, and finally put in calcium carbonate.

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Abstract

The invention provides a fermentation culture medium for improving enramycin yield. The content of organic carbon sources in the fermentation culture medium is 40-80 g / L; the content of organic nitrogen sources in the fermentation medium is 10-60 g / L. The invention also provides a method for fermentation production of enramycin, wherein the method comprises the steps of inoculating the fermentation culture medium with a streptomyces fungicidicus seed liquid, carrying out liquid fermentation, and after carrying out fermentation culture for 192-240 hours, extracting enramycin from the fermentation liquid. The fermentation culture medium and the fermentation method greatly improve the fermentation unit of enramycin, and are suitable for large-scale production of enduracidin.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a fermentation medium and a fermentation method for increasing the yield of enramycin. Background technique [0002] Enramycin is a polypeptide antibiotic composed of unsaturated fatty acids and more than a dozen amino acids, which is fermented by the actinomyces Streptomyces fungicidious NO.B5477 isolated from the soil. The drug was developed by Takeda Pharmaceutical Co., Ltd. in Japan in 1966, officially registered in Japan in 1974, and has since been registered and widely used in many countries. In 1993, the Ministry of Agriculture of my country approved the registration of the drug in my country. In 2005, a domestic manufacturer and Schering Plow Animal Health Corp. of the United States began to cooperate in the production of enramycin premix. Enramycin is an organic base composed of 17 amino acid molecules and fatty acid molecules of 13 different types. Among them, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04C12R1/465
Inventor 李荣杰张雪锋尚海涛徐斌杨为华邓远德张印
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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