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New purpose of foam killer

A defoamer and bacteria-producing technology, which is applied in the direction of foam dispersion/prevention, fermentation, etc., can solve the problems of affecting the oxygen mass transfer rate, causing serious consequences in the fermentation process, and affecting the physiological metabolism of antibiotic-producing bacteria.

Inactive Publication Date: 2009-10-21
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the defoamer can quickly defoam, it also inhibits the dispersion of bubbles, making the volumetric oxygen mass transfer coefficient K L a decreases, thereby significantly affecting the mass transfer rate of oxygen, causing the dissolved oxygen (DO) to drop rapidly or even to zero. If DO is lower than the critical value for a long time, it will have serious consequences for the fermentation process
Defoamers not only have the physical effect of defoaming, but also affect the physiological metabolism of antibiotic-producing bacteria, but most defoamers have a negative impact

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1 Materials and methods

[0053] 1.1 Strains

[0054] Bitespiramycin genetic engineering strain WSJ-1-195 was provided by Shenyang Tonglian Pharmaceutical Group Shanghai Research Institute.

[0055] 1.2 Culture medium formula (see Wang Yiguang, Jinlianfang, Jinwenzao, etc. Cloning of 4'acylase gene and expression in spiramycin-producing bacteria [J]. Acta Biological Engineering, 1992, 8(1): 1)

[0056] 1.2.1 Incline medium starch, soybean meal, NaCl, CaCO 3 , KH 2 PO 4 , peptone, etc.

[0057] 1.2.2 Seed medium starch, soybean meal, NaCl, CaCO 3 , KH 2 PO 4 , peptone, etc.

[0058] 1.2.3 Fermentation Shake Flask Medium Glucose, starch, fish meal, NH 4 NO 3 , CaCO 3 , KH 2 PO 4 , MgSO 4 ·7H 2 O, NaCl, etc.

[0059] 1.2.4 Medium for the determination of biological potency beef extract, peptone, glucose, yeast extract, agar, etc.

[0060] 1.3 Culture method

[0061] 1.3.1 Incline cultivation method

[0062] Add 40ml of slant medium to the eggplant bottle...

Embodiment 2

[0098] Physiological Effects in Fermentation of Erythromycin

[0099] The No. 12 defoamer in Example 1 verified the same effect in the fermentation of another macrolide antibiotic—erythromycin.

[0100] Operating conditions: Dig fresh slant (1cm×1cm) into a 500mL shake flask with 80mL seed medium, temperature 34°C, rotation speed 220r / min, after 44-48h of cultivation, transfer to a fermentation bottle for cultivation, inoculum size 5%, the temperature is 34°C, the rotation speed is 240r / min, and cultivated for 144h.

[0101] In the test group, different concentrations of No. 12 defoamer were added to the medium, and no defoamer was used as a control, and the biological potency was measured after the fermentation.

[0102] Image 6 It shows that adding 0.02% of the preferred antifoaming agent group increases the fermentation unit of erythromycin by 30.9%.

[0103] The results show that the No. 12 defoamer has a good effect on the defoaming and antifoaming of the bitspiramyci...

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Abstract

The invention discloses the functions of breaking foam, restraining foam and accelerating biosynthesis of polyether modification polysiloxane substance in the zymolysis process of macrolide antibiotic producing bacteria. The invention also discloses a use method of the polyether modification polysiloxane substance.

Description

technical field [0001] The invention relates to the field of microbial fermentation. More specifically it relates to new applications of defoamers in microbial fermentation. Background technique [0002] During the submerged fermentation of antibiotics, a large amount of foam will be formed due to stirring and aeration, and the protein, sugar and metabolites in the fermentation broth can stabilize the foam. Excessive foam has brought many adverse effects to fermentation, such as reducing the utilization rate of equipment and increasing the chance of contamination. When the foam is severe, it will also affect the normal operation of ventilation and stirring, resulting in abnormal metabolism. However, proper foam formation has a significant effect on the improvement of the oxygen transfer rate (OTR) of antibiotic fermentation. Therefore, screening a suitable defoamer to control the appropriate amount of foam is one of the important links in the antibiotic fermentation proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62B01D19/04
Inventor 郝玉有刘延茂王军峰庄英萍储炬王永红张嗣良
Owner EAST CHINA UNIV OF SCI & TECH
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