Semi-solid fermentation method of natamycin and natamycin extracting method

A semi-solid fermentation, natamycin technology, applied in the directions of fermentation, chemical instruments and methods, microorganism-based methods, etc., can solve the problems of instability of natamycin, affecting biological activity, and reducing extraction yield, etc. The extraction process is simplified, the yield is improved, and the material utilization rate is high.

Inactive Publication Date: 2012-05-09
武汉乐立基生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In strong acid and alkaline aqueous solutions, natamycin is unstable, and chemical reactions such as methylation or saponification will occur, destroying its structure, reducing its purity, and affecting its biological activity, resulting in a decrease in extraction yield.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Use the L-80 strain of Streptomyces luteus S.gilvosporeus as the production strain, and use Gao's No. 1 medium as the slant medium, inoculate and cultivate for 7 days, until the spore pile on the surface of the slant turns from white to grayish yellow, enter 4 ℃ refrigerator. Take it out after one week in the refrigerator, and prepare the spore suspension with sterile distilled water. The spores in the spore suspension are preferably about 100 million per milliliter.

[0048] The slant spores were cultured through secondary expansion to obtain seed liquid. The formula of the seed medium is peptone 1.2%, potassium chloride 0.8%, and glucose 1.8%. Glucose is sterilized separately at 115°C for 20 minutes. Before disinfection, the pH was 7.2. After disinfection, glucose was added in an aseptic manner, and the pH after mixing was 6.9. The primary seeds are packed with 25ml of seed liquid in a 250ml Erlenmeyer flask, cultivated at 29°C, 180rpm, on a large-amplitude shaker...

Embodiment 2

[0059] The Y-42 strain of S. gilvosporeus was used as the production strain. The slant culture medium is glucose 1.2%, yeast extract 0.4%, malt extract 0.3%, peptone 0.5%, agar 2.0%, glucose is sterilized separately, and the method is the same as above. Before disinfection, the pH value was 7.2. After inoculation with spores of the Y42 strain, it was cultured at a constant temperature of 28°C for 7 days, stored in a refrigerator at 4°C for 7 days, and then the spore suspension was made with sterile distilled water.

[0060] Secondary seed expansion is still used for seed expansion cultivation, and the seed medium formula is 2.0% glucose, 0.6% peptone, 0.6% corn steep liquor, 0.8% KCL, pH7.0. Glucose is sterilized separately, and the method is the same as above. The primary seeds were cultivated in a 50L seed tank, feeding 30L, at 28°C to 29°C, with a ventilation rate of 1:0.6 (V / V) and stirring at 220rpm, and cultivated for 24 hours. The secondary seeds are carried out in a ...

Embodiment 3

[0068]This example is carried out on a semi-solid fermentation bed. The used production strain is the L-333 strain of Streptomyces luteus S. gilvosporeus. The cultivation of its slant strain and the preparation of the spore suspension are the same as in Example 2.

[0069] Seed expansion cultivation adopts three-stage expansion cultivation method. The formulation of the seed medium is: 1.0% starch, 2.0% glucose, 1.5% bean cake powder (hot pressed), 0.2% corn steep liquor, 0.5% yeast powder, 0.03% potassium dihydrogen phosphate, and 0.8% light calcium carbonate. pH7.2 before disinfection. Glucose is sterilized separately and mixed with other substances after sterilization.

[0070] 500ml Erlenmeyer flask for primary seed culture. Pack 50ml of seed culture medium in the bottle, and inoculate 5ml of spore suspension of L-333 bacterial strain. Incubate at 29°C for 16 hours on a large-amplitude shaker at 200 rpm. Secondary seed culture is carried out in 2000ml Erlenmeyer flas...

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Abstract

The invention provides a semi-solid fermentation method of natamycin, which adopts porous particle-style substance as a carrier of fermentation substrate. The method comprises the steps of: adopting the porous particle-style substance as the carrier to absorb substrate substance to be inoculated with natamycin-producing strain for semi-solid fermentation; locally drying fermentation materials in air after the semi-solid fermentation is finished; and finally obtaining natamycin powder after processes of packing in column for dipping, leaching, exsolution, dilution precipitation and spraying-drying. The method for producing natamycin requires simple equipment, and the fermenting unit is high and the utilization rate of materials is also high; the extraction process avoids application of strong acid and strong alkali; and the yielding rate of natamycin is high and the method basically avoids discharge of wastewater and waste residue so as to meet the energy-saving and emission-reducing requirements of environment-protection.

Description

technical field [0001] The present invention relates to a fermentation extraction method of antibiotics, in particular to a new fermentation and extraction process of a novel food, beverage and feed preservative Natamycin. Background technique [0002] Natamycin is a broad-spectrum antifungal antibiotic. In June 1982, the U.S. FDA approved its use as a food additive. In 1996, my country approved natamycin to be used in cheese, meat products, cakes, fruit juices, and can also be used directly Add to fermented wine, yogurt and salad dressings. According to relevant experts, natamycin is the only highly effective and safe antifungal new biological source preservative widely recognized in the world. [0003] Natamycin is known to be fermentably produced by three species of Streptomyces, S. chattanovgensis, S. natalensis and S. gilvosporeus. Most of the Nata manufacturers in China produce with different production strains of Streptomyces chrysosporum. [0004] Since the earlies...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/62C07H17/08C07H1/06C12R1/465
Inventor 林开春王西平
Owner 武汉乐立基生物科技有限责任公司
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