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Culture medium and culture method for producing spinosad by fermenting saccharopolyspora spinosa

A technology of Saccharopolyspora spinosa and fermentation medium, applied in the field of biological fermentation, can solve the problems of high fermentation production cost, affecting the level of spinosad production strain fermentation technology, etc., and achieve the effect of improving fermentation units

Pending Publication Date: 2020-08-04
NINGXIA TAIYICIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because glucose is easy to carbonize under high temperature conditions, the culture medium containing glucose is prone to produce harmful substances after high temperature sterilization, which affects the fermentation technology level of spinosad production strains
[0006] 3 The carbon source in the spinosad medium is glucose, and the nitrogen source is mainly soybean cake powder, cottonseed cake powder, yeast powder or peptone or beef extract, resulting in high fermentation production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Primary seed culture:

[0065] Primary seed medium composition: molasses powder 30kg, soybean molasses 14kg, meat and bone meal 14kg, hydrolyzed feather meal 18kg, corn extract powder 20kg, ammonium sulfate 0.4kg, light calcium carbonate 1kg; add water to 1m 3 .

[0066] First, sterilize the primary seed medium and cool it to room temperature, control the pH at 6.4, and keep the pressure with sterile air, and then, under the protection of the flame, transfer the cultured Saccharopolyspora spinosa fermentation broth into the primary Carry out primary seed cultivation in seed medium, tank pressure 0.02-0.05MPa, tank temperature 27-28°C, stirring speed: 0-5h, use air stirring instead of mechanical stirring; 6h-transplanting: 60r / min; air flow rate 20m 3 / h, to 10% cell concentration, stop culturing.

[0067] Secondary seed culture:

[0068] Secondary seed medium composition: molasses powder 225kg, soybean molasses 90kg, meat and bone meal 80kg, hydrolyzed feather meal 9...

Embodiment 2

[0074] Primary seed culture:

[0075] Primary seed medium composition: molasses powder 31kg, soybean molasses 15kg, meat and bone meal 12kg, hydrolyzed feather meal 16kg, corn extract powder 18kg, ammonium sulfate 0.5kg, light calcium carbonate 2kg; add water to 1m 3 .

[0076] First, sterilize the primary seed medium and cool it down to room temperature, control the pH at 6.5, and keep the pressure with sterile air, and then, under the protection of the flame, transfer the cultured Saccharopolyspora spinosa fermentation broth into the primary Carry out primary seed cultivation in seed medium, tank pressure 0.02-0.05MPa, tank temperature 27-28°C, stirring speed: 0-5h, use air stirring instead of mechanical stirring; 6h-transplanting: 65r / min; air flow rate 25m 3 / h, to 12% cell concentration, stop culturing.

[0077] Secondary seed culture:

[0078] Secondary seed medium composition: molasses powder 200kg, soybean molasses 80kg, meat and bone meal 70kg, hydrolyzed feather m...

Embodiment 3

[0084] Primary seed culture:

[0085] Primary seed medium composition: molasses powder 33kg, soybean molasses 16kg, meat and bone meal 13kg, hydrolyzed feather meal 17kg, corn extract powder 19kg, ammonium sulfate 0.6kg, light calcium carbonate 3kg; add water to 1m 3 .

[0086] First, sterilize the primary seed medium and cool it to room temperature, control the pH at 6.6, and keep the pressure with sterile air, and then transfer the cultured Saccharopolyspora spinosa shake flask fermentation broth into the primary stage under flame protection. Carry out primary seed cultivation in seed medium, tank pressure 0.02-0.05MPa, tank temperature 27-28°C, stirring speed: 0-5h, use air stirring instead of mechanical stirring; 6h-transplanting: 70r / min; air flow rate 30m 3 / h, until the bacterial cell concentration is 15%, stop culturing.

[0087] Secondary seed culture:

[0088] The composition of secondary seed medium: molasses powder 210kg, soybean molasses 85kg, meat and bone mea...

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PUM

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Abstract

The invention relates to a culture medium and a culture method for producing spinosad through fermentation of saccharopolyspora spinosa. A carbon source, a nitrogen source and optimal compatibility ratio in a primary seed medium, a secondary seed medium and a fermentation medium for production of spinosad through fermentation of the saccharopolyspora spinosa in a three-stage fermentation mode areconfirmed. By utilizing the culture medium formula and the fermentation control process provided by the invention, the fermentation unit of rice spinosad can be improved, the fermentation cost is reduced, the sources of raw materials and auxiliary materials are furthest reduced and are not influenced by the environment, sufficient supply of the raw materials and the auxiliary materials is ensured,and stable and efficient production of the spinosad is realized.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a culture medium and a culture method for producing spinosyn by fermentation of Saccharopolyspora spinosa. Background technique [0002] Spinosyn is an intracellular secondary metabolite of aerobic fermentation of Saccharopolyspora spinosa, and the finished product is a white or off-white solid crystal, which is a mixture of spinosad A component and spinosad D component. Spinosyn is a new type of broad-spectrum biopesticide with contact and ingestion toxicity, and it is a microbial-derived biochemical pesticide. Due to the characteristics of low toxicity and low residue of spinosyn, the U.S. Environmental Protection Agency classified spinosyn as a low-risk pesticide product, and awarded it the "Presidential Green Chemical Challenge Award" in 1999. At present, spinosad has become a domestic research and development hotspot. [0003] At present, the th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/62C12R1/01
CPCC12N1/20C12P19/62
Inventor 陈德刚张萍
Owner NINGXIA TAIYICIN BIOTECH CO LTD
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