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Culture medium and culture method for fermentation producing spinetoram by utilizing saccharopolyspora spinosa

A technology of spinosyn and fermentation medium, which is applied in the field of fermentation, can solve the problems of weak competitiveness, low fermentation level, lack of literature reports on large-scale large-scale production of spinosad fermentation technology, etc., and achieve an increase in fermentation units , the effect of reducing fermentation energy consumption

Inactive Publication Date: 2017-05-24
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1 There is a lack of literature reports on the fermentation process of large-scale production of spinosad in China, which cannot provide effective technical support for large-scale production models
[0005] 2 The fermentation level of industrialized large-scale production is low, and its fermentation level is maintained at about 1-2g / L, resulting in weak competitiveness of products in the international market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Primary seed culture: the volume of seed medium is 100L.

[0055] Starch molasses 500g, bran 800g, olive oil 200g, fish meal 300g, corn steep liquor dry powder 1100g, neutral soybean peptone 400g, light calcium carbonate 100g.

[0056] The quality of the primary seed medium after sterilization is: amino nitrogen 20.4mg / L, reducing sugar 2.1g / 100ml.

[0057] The primary seed cultivation conditions are: tank pressure 0.03-0.06MPa; tank temperature 29-30°C; air flow: 30m 3 / h; stirring speed 60r / min.

[0058] The cultivation of the primary seed medium is completed, the bacterial cell concentration is 28%, and the cultivation time is 50h.

[0059] Secondary seed culture: the volume of seed medium is 500L.

[0060] Starch molasses 3500g, bran 5500g, olive oil 1500g / L, fish meal 2500g, corn steep liquor dry powder 6000g / L, neutral soybean peptone 2500g, biuret 100g, light calcium carbonate 1000g; potassium dihydrogen phosphate 15g.

[0061] The quality of the secondary seed...

Embodiment 2

[0080] Primary seed culture: the volume of seed medium is 100L.

[0081] Starch molasses 600g, bran 900g, olive oil 300g, fish meal 400g, corn steep liquor dry powder 1200g, neutral soybean peptone 500g, light calcium carbonate 200g.

[0082] The quality of the primary seed medium after sterilization is: amino nitrogen 22.1mg / L, reducing sugar 2.3g / 100ml.

[0083] The primary seed cultivation conditions are: tank pressure 0.03-0.06MPa; tank temperature 29-30°C; air flow: 35m 3 / h; stirring speed 65r / min.

[0084] The cultivation of the primary seed medium was completed, the bacterial cell concentration was 29%, and the cultivation time was 51 hours.

[0085] Secondary seed culture: the volume of seed medium is 500L.

[0086] Starch molasses 4000g, bran 6000g, olive oil 2000g / L, fish meal 3000g, corn steep liquor dry powder 6500g / L, neutral soybean peptone 3000g, biuret 150g, light calcium carbonate 1500g; potassium dihydrogen phosphate 20g.

[0087] The mass of the seconda...

Embodiment 3

[0106] Primary seed culture: the volume of seed medium is 100L.

[0107] Starch molasses 700g, bran 1000g, olive oil 400g, fish meal 500g, corn steep liquor dry powder 1300g, neutral soybean peptone 600g, light calcium carbonate 300g.

[0108] The quality of the primary seed medium after sterilization is: amino nitrogen 24.8mg / L, reducing sugar 2.5g / 100ml.

[0109] The primary seed cultivation conditions are: tank pressure 0.03-0.06MPa; tank temperature 29-30°C; air flow: 40m 3 / h; stirring speed 70r / min.

[0110] The cultivation of the primary seed medium is completed, the cell concentration is 30%, and the cultivation time is 52h.

[0111] Secondary seed culture: the volume of seed medium is 500L.

[0112] Starch molasses 4500g, bran 6500g, olive oil 2500g / L, fish meal 3500g, corn steep liquor dry powder 7000g / L, neutral soybean peptone 3500g, biuret 200g, light calcium carbonate 2000g; potassium dihydrogen phosphate 25g.

[0113] The quality of the secondary seed medium...

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PUM

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Abstract

The invention relates to a culture medium and a culture method for fermentation producing spinetoram by utilizing saccharopolyspora spinosa. According to the culture medium and the culture method, a seed culture medium of fermentation producing spinetoram by utilizing saccharopolyspora spinosa is determined, and a carbon source, a nitrogen source and the best compatibility ratio in the fermentation culture medium are determined. According to the culture medium formula and the fermentation production control process provided by the invention, the fermentation unit of spinetoram can be improved, product quality is guaranteed, fermentation period is controlled to be within 170 hours and fermentation cost is reduced; and moreover, raw and auxiliary materials are not affected by the environment to the greatest extent, so that sufficient supply is guaranteed, and stable and efficiency production of spinetoram is realized.

Description

technical field [0001] The invention belongs to the field of fermentation technology, in particular to a culture medium and a culture method for producing spinosad by fermentation of Saccharopolyspora spinosa. Background technique [0002] Spinosad is produced from the fermentation of the actinomycete Saccharopolyspora spinosa, and is mainly used to control Lepidoptera pests (Plutella xylostella, Beet Spodoptera) and Thysanoptera pests (Thrips). Spinosad not only has all the functions of spinosad in controlling pests, but also can play an excellent effect on spinosad when it has no significant effect in controlling crop pests such as fruits and nuts. The usage of spinosyn is lower than that of the pesticides currently on the market, and its residence time in the environment is shorter than that of the old pesticides; this excellent environmental characteristic will bring it a new The market is an extremely important supplement to the spinosad product sales market. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12R1/01
CPCC12P19/62
Inventor 任勇
Owner 宁夏泰瑞制药股份有限公司
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