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Culture medium for fermenting and producing rifamycin by virtue of Amycolatopsis mediterranei and culture method

A Mediterranean pseudo-branchless, fermentation medium technology, applied in the field of fermentation, can solve the problems of weak competitiveness in the international market, long fermentation cycle, and high energy consumption

Inactive Publication Date: 2017-10-10
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1 Domestic rifamycin fermentation production unit is relatively low, 60m 3 The fermentation unit of the fermenter does not exceed 22000u / ml, and there is a certain gap with the international production technology level
[0005] 2 The production cycle of rifamycin is generally 200-220h, the fermentation cycle is longer and the energy consumption is higher
[0006] Based on the above reasons, the production cost of rifamycin is relatively high, and the international market competitiveness is weak

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Primary seed culture: seed medium volume 1m 3 .

[0042] Transformed molasses 13kg, fly maggot dry powder 12kg, cell protein 9kg, yeast self-dissolving powder FM901 8kg, light calcium carbonate 1kg, ammonium sulfate 0.5kg.

[0043] First sterilize the seed medium and cool it down to 20°C, keep the pressure with sterile air, and then put 500ml of the cultured Amycolatopsis mediterranean mother bottle fermentation broth into the primary seed medium under flame protection Proceed to seed culture.

[0044] The culture conditions are: tank pressure 0.03-0.06MPa; tank temperature 28-39°C; air flow: 40m 3 / h; stirring speed 50r / min.

[0045] Inoculate until the cell concentration is 40%, and the culture time is 65h.

[0046] Secondary seed culture: seed medium volume 10m 3 .

[0047] Transformed molasses 160kg, fly maggot dry powder 140kg, bacterial protein 110kg, yeast self-dissolving powder FM901 50kg, microbial compound nitrogen FT100 80kg, light calcium carbonate 10k...

Embodiment 2

[0062] Primary seed culture: seed medium volume 1m 3 .

[0063] Transformed molasses 14kg, fly maggot dry powder 13kg, cell protein 10kg, yeast self-dissolving powder FM901 9kg, light calcium carbonate 2kg, ammonium sulfate 0.6kg.

[0064] First sterilize the seed medium and cool it down to 22°C, keep the pressure with sterile air, and then put 520ml of the cultured Amycolatopsis mediterranean mother bottle fermentation broth into the primary seed medium under flame protection Proceed to seed culture.

[0065] The culture conditions are: tank pressure 0.03-0.06MPa; tank temperature 28-39°C; air flow: 45m 3 / h; stirring speed 53r / min.

[0066] Inoculate until the cell concentration is 41%, and the culture time is 66 hours.

[0067] Secondary seed culture: seed medium volume 10m 3 .

[0068] Transformed molasses 170kg, fly maggot dry powder 150kg, cell protein 120kg, yeast self-dissolving powder FM901 60kg, microbial compound nitrogen FT100 90kg, light calcium carbonate 11...

Embodiment 3

[0083] Primary seed culture: seed medium volume 1m 3 .

[0084] Transformed molasses 15kg, fly maggot dry powder 14kg, cell protein 11kg, yeast self-dissolving powder FM901 10kg, light calcium carbonate 3kg, ammonium sulfate 0.7kg.

[0085] First, sterilize the seed medium and cool it to 23°C, keep the pressure with sterile air, and then put 550ml of the cultured Amycolatopsis mediterranean mother bottle fermentation broth into the primary seed medium under flame protection Proceed to seed culture.

[0086] The culture conditions are: tank pressure 0.03~0.06MPa; tank temperature 28~39℃; air flow: 50m 3 / h; stirring speed 55r / min.

[0087] Inoculate until the cell concentration is 42%, and the culture time is 67h.

[0088] Secondary seed culture: seed medium volume 10m 3 .

[0089] Transformed molasses 180kg, fly maggot dry powder 160kg, cell protein 130kg, yeast self-dissolving powder FM901 70kg, microbial compound nitrogen FT100 100kg, light calcium carbonate 12kg, ammo...

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PUM

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Abstract

The invention relates to a culture medium for fermenting and producing rifamycin by virtue of Amycolatopsis mediterranei and a culture method. According to the culture medium and the culture method, a carbon source, a nitrogen source and an optimal compatibility proportion in a seed culture medium and a fermentation culture medium for producing rifamycin through fermentation of Amycolatopsis mediterranei are determined. By utilizing a culture medium formula and a fermentation production control process provided by the invention, the level of a fermentation technique of rifamycin can be increased, the fermentation cost is lowered, the sources of raw and auxiliary sources are not influenced by the environment, the adequate supply of the raw and auxiliary sources is guaranteed, and the stable and efficient production of rifamycin is realized.

Description

technical field [0001] The invention belongs to the field of fermentation technology, and in particular relates to a culture medium and a culture method for fermenting and producing rifamycin by using Amycolatopsis mediterranei. Background technique [0002] Rifamycins are a class of anssa macrolide antibiotics produced by Amycolatopsis mediterranei. These antibiotics have low toxicity, high efficacy and broad antibacterial spectrum, and are widely used in the treatment of G+ (Gram-positive bacteria), tuberculosis, leprosy and AIDS-related mycobacterial infections. [0003] Since the discovery of rifamycin in the late 1950s, a lot of research has been done on its structure and properties and its fermentation process, and the fermentation unit has been greatly improved. However, in recent years, the industrialization level of rifamycin in my country has increased slowly. The existing problems are mainly: [0004] 1 Domestic rifamycin fermentation production unit is relativel...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12N1/20C12R1/01
CPCC12P17/189C12N1/20
Inventor 赵海兵王大伟
Owner 宁夏泰瑞制药股份有限公司
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