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Novel culture medium and culture method for fermentation production of mitomycin through Streptomyces caespitosus

A fermentation medium and mitomycin technology, which is applied in the field of fermentation, can solve the problems of less mitomycin, affecting the quality of fermentation broth, reducing total sugar content and the like

Active Publication Date: 2016-03-30
HEILONGJIANG LIANSHUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2 There are few domestic and foreign reports on the production process of mitomycin
[0006] 3 Glucose is added to the medium for fermenting and producing mitomycin, and after high-temperature sterilization, it is easy to cause part of the glucose to be carbonized, reduce the total sugar content, and affect the quality of the fermentation broth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Seed culture: the effective volume of the seed tank is 1m 3 .

[0057]The composition of the seed medium is: mannitol 4kg, sugarcane molasses 8kg, maltose 5kg, maltase 0.02kg, silkworm chrysalis powder 4kg, corn steep liquor 10L, corn gluten powder 3kg, ammonium sulfate 3kg, light calcium carbonate 1kg. Quality of sterilized seed medium: amino nitrogen 42 / 100ml, reducing sugar 51g / L, pH: 7.4.

[0058] Sterilize the seed medium and cool it to 25-30°C, keep the pressure with sterile air, and then under the protection of the flame, ferment the cultured Streptomyces capitus mother bottle fermentation liquid at a rate of 0.5L / m 3 The inoculum amount was inserted into the seed medium for seed culture.

[0059] Seed culture conditions are: tank pressure 0.03-0.05MPa; tank temperature 28-29°C; air flow: 0-10h, using air stirring instead of mechanical stirring; 11h-transplanting: 30m 3 / h; stirring speed 70r / min. Transplant when the cell concentration is 35%, the pH is 6.8, ...

Embodiment 2

[0074] Seed culture: the effective volume of the seed tank is 1m 3 .

[0075] The composition of the seed medium is: mannitol 5kg, sugarcane molasses 9kg, maltose 6kg, maltase 0.03kg, silkworm chrysalis powder 5kg, corn steep liquor 11L, corn gluten powder 4kg, ammonium sulfate 4kg, light calcium carbonate 2kg. The quality of the sterilized seed medium: amino nitrogen 45 / 100ml, reducing sugar 56g / L, pH: 7.2;

[0076] Sterilize the seed medium and cool it down to 25-30°C, keep the pressure with sterile air, and then, under the protection of the flame, ferment the cultured Streptomyces capitus mother bottle fermentation broth at a rate of 0.6L / m 3 The inoculum amount was inserted into the seed medium for seed culture.

[0077] The seed culture conditions are: tank pressure 0.03-0.05MPa; tank temperature 28-29°C; air flow: 0-10h, using air stirring instead of mechanical stirring; 11h-transplanting: 35m 3 / h; stirring speed 72r / min. Transplant when the cell concentration is 36...

Embodiment 3

[0092] Seed culture: the effective volume of the seed tank is 1m 3 .

[0093] The composition of the seed medium is: 6kg of mannitol, 10kg of sugarcane molasses, 7kg of maltose, 0.04kg of maltase, 6kg of silkworm chrysalis powder, 12L of corn steep liquor, 5kg of corn gluten powder, 5kg of ammonium sulfate, and 3kg of light calcium carbonate. The quality of the sterilized seed medium: amino nitrogen 51 / 100ml, reducing sugar 61g / L, pH: 7.0;

[0094] Sterilize the seed medium and cool it down to 25-30°C, keep the pressure with sterile air, and then under the protection of the flame, ferment the cultured Streptomyces capitus mother bottle fermentation broth at a rate of 0.7L / m 3 The inoculum amount was inserted into the seed medium for seed culture.

[0095] The seed culture conditions are: tank pressure 0.03-0.05MPa; tank temperature 28-29°C; air flow: 0-10h, using air stirring instead of mechanical stirring; 11h-transplanting: 40m 3 / h; stirring speed 75r / min. Transplant wh...

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Abstract

The invention relates to a novel culture medium and a culture method for fermentation production of mitomycin through Streptomyces caespitosus. By the aid of the formula of the culture medium and the fermentation control technology, the fermentation unit of the mitomycin can be increased, the fermentation cycle can be shortened, the fermentation cost can be reduced, the influence caused by raw and auxiliary materials on the fermentation unit is reduced to the greatest extent, the production cost is reduced, sources of the raw and auxiliary materials are not affected by the environment, the raw and auxiliary materials are sufficiently supplied, and stable and efficient production of the mitomycin is realized.

Description

technical field [0001] The invention belongs to the field of fermentation technology, and in particular relates to a novel culture medium and a culture method for producing mitomycin by fermentation of Streptomyces capitis. Background technique [0002] Mitomycin (Mitomycin) is a broad-spectrum anti-tumor antibiotic. Since Hata was the first to isolate Mitomycin from the culture fluid of Streptomyces capitulum in 1956, it has been widely used due to its special anti-tumor effect. focus on. [0003] At present, the domestic fermentation production of mitomycin adopts the second fermentation mode, and the main problems are as follows: [0004] 1 The fermentation level of mitomycin is low, generally controlled at around 800u / ml. [0005] 2 There are few domestic and foreign reports on the production process of mitomycin. [0006] 3 Adding glucose to the culture medium for fermenting and producing mitomycin, after high-temperature sterilization, can easily lead to carbonizati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12N1/20C12R1/465
CPCC12N1/20C12P17/182
Inventor 李小萍
Owner HEILONGJIANG LIANSHUN BIOTECHNOLOGY CO LTD
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