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Culture medium and culture method for producing coenzyme Q10 by fermentation of rhodobacter sphaeroides

A technology of Rhodobacter sphaeroides and fermentation medium, which is applied in the field of biological fermentation, can solve the problems of high cost of medium formula, affecting the fermentation technology level of coenzyme Q10 production strains, etc., and achieves the effect of increasing fermentation units and reducing costs.

Inactive Publication Date: 2021-01-22
NINGXIA TAISHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because glucose is easy to carbonize under high temperature conditions, the culture medium containing glucose is prone to produce harmful substances after high temperature sterilization, which affects the fermentation technology level of coenzyme Q10 production strains
[0006] 3 The carbon source in the coenzyme Q10 medium is glucose, and the nitrogen source is mainly soybean powder, yeast extract, peptone or beef extract, and the cost of the medium formula is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Primary seed culture: primary seed liquid volume 1m 3

[0070] The composition of primary seed medium: molasses fermentation liquid 20kg, primary peanut meal 19kg, alfalfa powder 8kg, DDGS 13kg, ammonium sulfate 0.4kg, light calcium carbonate 1kg.

[0071] Primary seed culture: first sterilize the primary seed medium and cool it to room temperature, control the pH at 6.0, and keep the pressure with sterile air, then shake the cultured Rhodobacter sphaeroides 1L in the flask under flame protection Connect to the primary seed medium for primary seed cultivation, tank pressure 0.02-0.06MPa; tank temperature 31-32°C; stirring speed: 120r / min; air velocity: 100m 3 / h. Transplant when the OD value reaches 3.1.

[0072] Secondary seed culture: Secondary seed liquid volume 10m 3

[0073] The composition of secondary seed medium: 220kg of molasses fermentation liquid, 210kg of primary peanut meal, 100kg of alfalfa powder, 150kg of DDGS, 70kg of silkworm chrysalis protein, 5...

Embodiment 2

[0089] Primary seed culture: primary seed liquid volume 1m 3

[0090] The composition of primary seed medium: 21kg of molasses fermentation broth, 20kg of primary peanut meal, 9kg of alfalfa powder, 14kg of DDGS, 0.5kg of ammonium sulfate, and 1.5kg of light calcium carbonate.

[0091] Primary seed culture: first sterilize the primary seed medium and cool it to room temperature, control the pH at 6.2, and keep the pressure with sterile air, then shake the cultured Rhodobacter sphaeroides with 1.2 L is connected to the primary seed medium for primary seed cultivation, the tank pressure is 0.02-0.06MPa; the tank temperature is 31-32°C; the stirring speed: 125r / min; the air velocity: 130m 3 / h. Transplant when the OD value reaches 3.3.

[0092] Secondary seed culture: Secondary seed liquid volume 10m 3

[0093] The composition of secondary seed medium: molasses fermentation liquid 230kg, primary peanut meal 220kg, alfalfa powder 110kg, DDGS 160kg, silkworm chrysalis protein ...

Embodiment 3

[0109] Primary seed culture: primary seed liquid volume 1m 3

[0110] The composition of primary seed medium: molasses fermentation liquid 22kg, primary peanut meal 21kg, alfalfa powder 10kg, DDGS 15kg, ammonium sulfate 0.6kg, light calcium carbonate 2kg.

[0111] Primary seed culture: first sterilize the primary seed medium and cool it down to room temperature, control the pH at 6.4, and keep the pressure with sterile air, then under the protection of the flame, shake the cultured Rhodobacter sphaeroides with 1.5 L is connected to the primary seed medium for primary seed cultivation, the tank pressure is 0.02-0.06MPa; the tank temperature is 31-32°C; the stirring speed: 135r / min; the air velocity: 150m3 / h. Transplant when the OD value reaches 3.5.

[0112] Secondary seed culture: Secondary seed liquid volume 10m 3

[0113] The composition of secondary seed medium: 240kg of molasses fermentation liquid, 230kg of primary peanut meal, 120kg of alfalfa powder, 170kg of DDGS,...

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Abstract

The invention relates to a culture medium and a culture method for producing coenzyme Q10 by fermentation of rhodobacter sphaeroides. According to the invention, the carbon source, the nitrogen sourceand the optimal compatibility ratio in the primary seed culture medium, the secondary seed culture medium and the fermentation culture medium for producing the coenzyme Q10 by fermentation of rhodobacter sphaeroides in a three-stage fermentation mode are confirmed. By utilizing the culture medium formula and the fermentation control process provided by the invention, the fermentation unit of thecoenzyme Q10 can be improved, the fermentation cost is reduced, and the stable and efficient production of the coenzyme Q10 is realized.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, in particular to a culture medium and a culture method for producing coenzyme Q10 by fermenting Rhodobacter sphaeroides. Background technique [0002] Coenzyme Q10 is a fat-soluble antioxidant that can activate the nutrition of human cells and cell energy. It has the functions of improving human immunity, enhancing anti-oxidation, delaying aging and enhancing human vitality. It is widely used in medicine for diseases of the cardiovascular system. It is widely used in nutritional health products and food additives. [0003] At present, the fermentation production of coenzyme Q10 in China adopts a three-stage fermentation mode. The process is as follows: the coenzyme Q10 production strain is cultured in a shake flask, connected to the first-level seed tank for cultivation, and transferred to the second-level seed tank after meeting the transplanting conditions Cultivate, transplant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/66C12N1/20C12Q3/00C12R1/01
CPCC12P7/66C12N1/20C12Q3/00
Inventor 任勇李小萍
Owner NINGXIA TAISHENG BIOTECH CO LTD
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