54 results about "Streptomyces auratus" patented technology

The invention relates to a method for producing tetracycline by fermentation of streptomyces aureus. The method comprises the following steps: slant spore culture, seed culture and fermentation culture of the streptomyces aureus, and is characterized in that dextrin, sucrose, corn pulp, hydrolyzed soybean meal and other medium-effect carbon-nitrogen sources are adopted to replace original late-effect carbon-nitrogen sources; by reducing the contents of starch and bean flour in an original fermentation culture medium and combining with a formula of a mixture in the fermentation process and adjustment of a replenishing method and placing part of the nitrogen sources in the fermentation culture medium into material replenishment in the fermentation process, the concentration of the culture medium in the fermentation process is effectively controlled, the oxygen dissolution condition in the early stage of fermentation is improved, the final residue of the culture medium of fermentation is controlled by material replenishment, the material consumption is reduced, and the emission of solid wastes is reduced; the metabolism velocity in exponential growth phase in the fermentation process of the tetracycline is effectively controlled, the abnormal situations that a fermentation solution turns red and becomes sour and the like in the fermentation process are reduced, and the fermentation level is improved.

The invention provides a preparation method of technical grade kasumin, which comprises the following steps: filtering a Streptomyces ricroaureus fermentation liquid to remove hyphae, treating the filtrate with hydrochloric acid to regulate the pH value to 2-7, concentrating the filtrate, adding a carrier into the filtrate, pulping under a paddle stirring condition, and carrying out spray drying. By omitting resin impurity removal, ammonium removal and other steps, and carrying out spray drying after adding the carrier into the filtrate and pulping, the method overcomes the defect of low technical grade kasumin yield caused by the resin impurity removal process, and avoids the melting and bonding caused by direct spray drying without resin impurity removal, thereby successfully improving the kasumin yield. Because of the adsorption and dilution effects of the carrier, the preparation method ensures that the product subjected to spray drying has fine fluidity; dry and uniform powder can be formed, and is beneficial to the subsequent preparation; and the production process is simple and practicable.

The invention discloses an organic microbial fertilizer for lettuces and a preparation method thereof. The organic microbial fertilizer for lettuces is prepared from the following components in parts by weight: 4-7 parts of streptomyces aureus, 0.6-0.9 part of bacillus thuringiensis, 3-5 parts of enzyme bacteria, 900-1100 parts of waste liquid precipitates, 240-380 parts of an inorganic fertilizer, 45-55 parts of trace elements, 25-32 parts of an adhesive, 0.5-0.7 part of a stabilizer and 0.1-0.3 part of an effect-stabilizer assistant. The organic microbial fertilizer prepared by the method is capable of increasing the yield of the lettuce, the content of vitamin C and the content of an iron element; and the content of nitrate is reduced, so that the quality of the lettuce is enhanced greatly. Compared with other fertilizers containing the same nutrients, the organic microbial fertilizer is capable of decreasing or lowering crop disease-pest damages which affect the crop quality. The using quantity of a chemical fertilizer is not decreased, and the organic microbial fertilizer is beneficial to environmental protection.

The invention relates to a kasugamycin water-aqua extracting process, which comprises the following steps: carrying out plate-frame filtering on streptomyces microaureus fermentation liquor so as to remove mycelia; carrying out continuous centrifugalization on the filtrate; feeding the obtained centrifugate into an ion-exchange column and carrying out ion-exchange, and desorbing the obtained product at room temperature by using ammonium chloride saturated solution; starting a nanofiltration device to carry out ammonium removal treatment; concentrating the filtrate, so that the kasugamycin content is 0.5-4%; and sequentially carrying out re-filtrating, color blending and packaging on the obtained product. Compared with original processes, according to the invention, continuous centrifugalization is newly increased for removing undissolved substances left out by plate-frame filtering, thereby saving a process of rinsing by using a plenty of water, shortening the production cycle, and reducing the production cost; according to the invention, a step of ammonium removal by using a nanofiltration membrane is added, so that ammonium salts in desorption liquid can be effectively and quickly removed, and then used for recycling; and kasugamycin is subjected to certain-procedure concentration, so that the concentration efficiency is increased, the product appearance is improved, and the product quality is enhanced.

The invention discloses a method for high throughput screening of chlortetracycline high-yield strains and belongs to the technical field of metabolic engineering. Through a comparison of growth and yield of streptomyces aureofaciens on five flat plate culture mediums, an optimal culture medium is selected, and guarantee is provided for starting strains. Through a comparison of two primary screening methods which are color reaction between chlortetracycline and Sm3+ and a colorimetric method of co-heat of chlortetracycline and hydrochloric acid, the simple and accurate color reaction is selected as the primary screening method. Repeatability and accuracy of nitric acid color reaction serving as a primary screening means are verified.

The invention relates to the field of fermentation production processes, and discloses a chlortetracycline fermentation production method using refined protein powder. According to the present invention, Streptomyces aureus is adopted as a starting strain, and pure culture and two-stage fermentation are performed to produce chlortetracycline; with the application of the 100% refined protein powder to replace the soybean cake powder, the chemical titer is increased by 2.3% compared to the application of the soybean cake powder, and the yield is increased by 1.8% compared to the unimproved method, such that the yield is effectively increased; and the raw material cost input per tank can be reduced by about 540 yuan after the cost accounting, the effect is good, the fermentation production level is stably increased, and the theoretical and applied basis is provided for the industrial production of the antibiotics and the scientific research.

The invention relates to microbial fertilizer as well as a preparation method and application thereof, and belongs to the field of comprehensive control of plant soil-borne diseases. The microbial fertilizer comprises a microbial agent, a nutritional agent, a carrier and water; the microbial agent comprises bacillus laterosporus, streptomyces jingyangensis, streptomyces lavendulae, streptomyces aureofaciens, bacillus mucilaginosus and trichoderma viride. The microbial fertilizer provided by the invention contains functional microorganisms capable of inhibiting and killing pathogenic bacteria in soil, can effectively prevent and treat tomato canker by a method for inhibiting bacteria by bacteria and can improve the soil healthy environment; germs can be killed from the soil root, various ways of seeds, seedlings, irrigating water, farm tools and the like can be blocked, and pathogen transmission can be avoided; the microbial fertilizer adopts natural microorganisms in the natural world,is green and free of pollution and has long-term effect; the use of pesticide is reduced, the planting cost is reduced and environmental pollution is reduced.

The invention discloses a keratin producing bacterial strain as well as culture conditions and crude enzyme property thereof and belongs to the technical field of microorganisms. Through identification, the bacterial strain is Streptomyces aureofaciens, named as K13 and preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number of CGMCC NO.8047. Through preliminary optimization, the culture medium of the bacterial strain comprises 0.5-1% of wool, 4-8% of glucose, 3-5% of peptone, 0.3% of sodium nitrate, 0.04% of dipotassium phosphate and 0.05% of sodium chloride; the shake culture conditions are that the initial pH (potential of Hydrogen) value is 10.0, the inoculation quantity is 10%, the culture temperature is 30 DEG C, the rotating speed is 220 revolutions per minute, the liquid loading volume is 30ml/250ml, the fermentation period is 64 hours and the keratinase producing activity is up to 44.2U/ml. The keratinase is mesophilic alkaline keratinase, the optimum operation temperature is 50-60 DEG C and the optimum pH is 8.5; the enzyme activity of the keratinase is stable in an alkaline environment (pH 7.0-10.0).

The invention relates to a method for increasing the biological value of kasugamycin. A small Streptomyces aureus ordinary culture medium is composed of soybean extraction powder, NaCl, corn steep liquor dry powder, maltose, fish oil, KH2PO4 and inositol, and an optimized culture medium is formed by adding growth factors and trace elements on the basis of the ordinary culture medium. Small Streptomyces aureus is cultured in the culture medium where the growth factors and the trace elements are added, the biological value of kasugamycin is increased by 19.7%, and the yield of single tank is improved by 10.8%. Small Streptomyces aureus cultured by utilizing the culture medium can obviously improve the biological value and the yield of single tank of kasugamycin.

The invention relates to a method for breeding high-yielding strain of tetracycline. The method is characterized by comprising the steps of: adding water into cultured Streptomyces aureus strains to prepare a spore suspension; shaking the flask for cultivation; filtrating; diluting; filling the suspension in four test tubes; adding a same amount of an EDTA solution and acting for respectively 3, 4, 5 and 7min; adding lysozyme and acting for 3 min; conducting gradient dilution to 10<-5>; and coating 0.1, 0.3 and 0.5ml spore liquid from each dilution on plates for cultivation; calculating a forward-mutation rate and a mortality rate; selecting an acting time with high forward-mutation rate; conducting further flask shaking screening; and selecting strains with high increase rate. The invention adopts a method, which first uses lysozyme to remove cell wall and then conducts EDTA treatment, thus breeding high-quality and high-yielding strain of tetracycline; and compared with an ultraviolet method and an ion implantation method, the method provided by the invention has advantages of simple operation, low cost, high safety, little environmental pollution, high mutation rate of strains, and high shake flask titer of the produced strain.

The invention discloses streptomycete bacterial strains and a combined application of the streptomycete bacterial strains for preventing and treating sclerotinia rot of colza and belongs to the technical field of microorganisms. The actinomycetes are separated from a 10-15cm deep soil sample collected in saline-alkali soil in suburb of Taian City, Shandong Province by using an actinomycetes separating technique. Authenticated by microbial taxonomy, the strains are named as streptomyces rimosus M527 and streptomyces aureofaciens M930, wherein S.rimosus M527 is preserved in China Center For Type Culture Collection on June 19th, 2013 with the preservation number of CCTCCNO: M2013270; S.aureofaciensM930 is preserved in the CCTCC on December 25th, 2013 with the preservation number of CCTCCNO:M2013711. The two bacterial strains can ferment to generate active substances which have relatively strong antagonism to sclerotinia sclerotiorum(Lib.)debary, and can be used for preventing and treating sclerotinia rot of colza. When the fermenting products of the two bacterial strains are mixed in a weight ratio of 4 to 1 to prepare a mixed preparation, the effect for preventing sclerotinia rot of colza is more remarkable than that of the two metabolites which are respectively used as single preparations.

The invention relates to a mutagenesis and screening method of sterptomyces kasugaensis efficiently producing kasugamycin, belonging to the technical field of microorganisms. The method utilizes a newatmospheric and room-temperature plasma (ARTP) breeding system to mutagenize a strain producing kasugamycin, and mainly includes the following steps: (1) slant culture of sterptomyces kasugaensis andcounting of slant spores; (2) preparation of spore suspension; (3) mutagenesis of sterptomyces kasugaensis by atmospheric and room-temperature plasma (ARTP); and (4) screening of a strain efficientlyproducing kasugamycin. The highest potency of shaking flask fermentation of sterptomyces kasugaensis screened by the method can reach 5091 [mu]g/mL, which is 130% higher than that of the original strain. The method has important economic values in industrial production.

The invention discloses a preparation method of tetracycline based on fermentation effect improvement. The preparation method includes the following steps that 1, fermentation liquor is prepared: golden streptomyces aureus spores are inoculated to a spore culture medium for culture for 5 days, then the prepared spores are transferred to a seeding tank for culture, and the prepared spores undergo fermentation culture after seed culture is completed; 2, the fermentation liquor is acidified; 3, the separation and purification of the fermentation liquor is are performed: filtration treatment is conducted on the acidified filtrate by using a plate frame, the filtrate obtained after filtration is decolored by using 122 ion exchange resin till the light transmittance of the filtrate is up to 90% or above, the filtrate is fed into a crystallizing tank for crystallization, then centrifugal separation is performed till the water content of wet crystals obtained after separation is up to 20% by mass percentage concentration, the wet crystals obtained through centrifugation are dried, and finally dried powder with the water content of 10% or below by mass percentage concentration is obtained. The preparation method includes few steps, reaction conditions are mild, industrialized production of tetracycline hydrochloride is easy to achieved, and a new thought is provided for production of the tetracycline hydrochloride.

The manufacturing method of biopesticide is characterized as follows: (1) the streptomyces aureus bacterial spawn is first stage cultured in first stage fermentation tank with air volume 1:0.3-0.6 and stirring 200-250 rpm, temperature 27-30 deg.C for 24-36 hrs; (2) and stage cultured with air volume 1:0.3-0.6, stirring 180-210 rpm, temperature 27-30 deg.c for 28-32 hr; (3) third stage cultured with culture dedium pH 7.2-7.4, air volume 1:0.2-0.4, stirring 160 rpm for 48-50 hr.. The invented biopesticide is of high effective, no poison, no hazard etc.

The invention discloses a method for producing aureomycin by using streptomyces aureus. According to the method, a culture medium and a culture method are combined and optimized, so that the synthesis and accumulation of aureomycin are promoted, and the proportion of impurity tetracycline and demethyl aureomycin is further reduced. A high-yield strain breeding model is constructed based on multiple composite mutagenesis and precursor/product tolerance, so that the diversity of a mutation library is higher, the method is simple, convenient and rapid, the screening efficiency is improved, the screening workload is reduced, and the method has certain application value and guiding significance for the reduction of the production cost and the improvement of the productivity of aureomycin andother streptomyces-derived antibiotics. The mutant strain can efficiently synthesize and accumulate aureomycin, the content of the impurity tetracycline and demethyl aureomycin is remarkably reduced, the genetic stability is good, the form, color, aureomycin production level and the content of main impurities are basically stable after continuous passage for five generations, and the mutant strain can be applied to industrial fermentation production and can be used as a production strain for further research and development.

The invention discloses a streptomyces aureus mutant strain, a breeding method thereof and an optimized culture medium. The mutant strain can efficiently synthesize and accumulate aureomycin, the content of impurity tetracycline and demeclocycline is remarkably reduced, the genetic stability is good, the form, color, aureomycin production level and main impurity content of the mutant strain are basically stable after five generations of continuous passage, and the mutant strain can be applied to industrial fermentation production and can be used as a production strain for further research and development. The optimized culture medium is beneficial to promoting synthesis and accumulation of the aureomycin, and the proportion of the impurity tetracycline and demeclocycline is further reduced. A high-yield strain breeding model is constructed based on multiple compound mutagenesis and precursor/product tolerance, the diversity of a mutation library is higher, simpleness, convenience and quickness are achieved, the screening efficiency is improved, the screening workload is reduced, and certain application value and guiding significance for reducing the production cost and improving the productivity of the aureomycin and other streptomycete source antibiotics are achieved.

The invention relates to a preparation method and application of a rare earth luminescent complex. Through synthesis of (2, 2-triethoxysilylpropionamido) benzoyl-p-tolylsulfoxide, with (2, 2-triethoxysilylpropionamido) benzoyl-p-tolylsulfoxide as a ligand, an europium rare earth perchlorate solid binary complex Eu (pps-Si)2.(ClO4)3.6H2O is synthesized, the solid binary complex can be well used asa fluorescence indicator to detect whether mixed fungi pollution happens during a Kasugamycin production process by fermentation of Streptomyces microaureus, and the indicator has high sensitivity, simple operation and strong practicability. When the method is used for detecting mixed fungi during the Kasugamycin production process by fermentation of Streptomyces microaureus, the detection procedure is optimized, and the method has the characteristics of simple operation, strong practicability and the like.

The invention relates to a streptomyces aureus fermentation tetracycline production method. The streptomyces aureus fermentation tetracycline production method comprises the technical processes of dissolving and diluting original streptomyces aureus strains, inoculating and uniformly shaking the dissolved original streptomyces aureus strains into a spore culture medium for cultivation at 33-35 DEGC for 17-25 hours, and sequentially performing seed cultivation and fermented cultivation, wherein the spore culture medium is composed of 1.0-1.5% of bran, 0.003-0.006% of dipotassium phosphate, 1.0-1.6% of starch, 0.01-0.03% of magnesium sulfate, 0.3-0.8% of corn pulp, 0.03-0.06% of yeast powder and 0.01-0.03% of calcium carbonate. Compared with a slant culture manner in the prior art, the streptomyces aureus fermentation tetracycline production method performs fermented tetracycline production through a spore cultivation medium, and by culturing spores through the culture medium, overcomesthe influence factors of agar in a slant culture medium, thereby having the advantages of being free from influence of raw materials, easy in material acquisition and stable in quality; shake-flake seeds are inoculated into the strains cultured through spore solution to ensure robust and well-spread mycelium, and the cultured strains are high in adaptability to culture solutions of every grade, high in metabolism velocity when moved into a fermentation tank, early in tetracycline secretion, short in fermentation cycle and capable of greatly increasing the fermentation level.

The invention discloses a purification process of tetracycline. The purification process comprises the following steps: 1) preparing a tetracycline crude product: conducting fermentation cultivation of streptomyces aureus spores in a fermentation tank, adding oxalic acid and conducting acidifying treatment; conducting filtration so as to obtain a filtrate, adding calcium salt to the filtrate under an alkaline condition, conducting precipitation, and conducting acid pickling on a precipitate so as to obtain the tetracycline crude product; 2) drying the tetracycline crude product; 3) preparing a tetracycline-inorganic salt dissolved solution, namely, dissolving the dried tetracycline crude product by virtue of a saturated inorganic salt solution, so that the tetracycline-inorganic salt dissolved solution is obtained; and 4) precipitating the tetracycline-inorganic salt dissolved solution, namely, dropping ammonia water to the tetracycline-inorganic salt dissolved solution to regulate pH value to 3-5, decreasing temperature to 0-10 DEG C, keeping the dissolved solution for 1-2h until a tetracycline-inorganic salt precipitate is separated out, and then sequentially conducting vacuum filtration and vacuum drying treatment, so that a finished tetracycline product is obtained. The purification process provided by the invention has fewer steps, so that the loss of the tetracycline in a purifying process is reduced.

The invention discloses application of streptomyces aureus Tu117 as a marker for detecting and diagnosing hypertension to preparation of a corresponding detection tool. A detection object of the detection tool is excrement, intestinal tissues or intestinal contents collected from a subject. The streptomyces aureus Tu117 of the invention is relatively high in content in bodies of hypertension model mice and hypertension patients, can be used for establishing a detection method or preparing a detection and diagnosis kit, and is used for auxiliary diagnosis and risk assessment of hypertension and prehypertension.

The invention relates to a bevel solid culture medium and culture method for producing tetracycline through fermentation of streptomyces aureus. The culture medium is prepared from, by weight, 2.0-2.5% of bran, 0.005-0.008% of dipotassium phosphate, 0.008-0.013% of diammonium hydrogen phosphate, 0.003-0.006% of magnesium sulfate, 0.5-1.0% of corn steep liquor, 0.003% of calcium carbonate, 1.8-2.5%of agar and the balance water. By changing the raw material composition in the streptomyces aureus bevel solid culture medium, the defect that culture quality fluctuation is caused by raw material quality fluctuation in the original formula is overcome, it is ensured that the prepared bevel spores are stable in both appearance and culture activity, and the fermentation level is also stably improved.

The invention provides kasugamycin raw powder, and a preparation method and application thereof. The preparation method of the kasugamycin raw powder comprises the following steps: carrying out shake-flask culture, primary seeding tank fermentation culture, secondary seeding tank fermentation culture and fermentation tank fermentation culture on streptomyces microaureus strains; and adjusting thepH value of the obtained fermentation product with an acid solution, performing tank sterilization and filtering, performing evaporating to obtain an aqueous kasugamycin solution, adding sodium benzoate, carrying out concentrating, and then carrying out spray drying and crushing to obtain the kasugamycin raw powder. The kasugamycin raw powder prepared by the invention is prepared by fermenting streptomyces microaureofaciens step by step and strictly controlling a production process; the kasugamycin raw powder is high in content, high in quality, safe to use and free of toxic and side effects;and the preparation method is simple and efficient. The kasugamycin raw powder prepared by the invention can be used for preventing and treating plant pathogenic bacteria and preparing a purifying agent for purifying an aquaculture water body, and has wide application prospects.

The present invention relates to a method for inoculation by adopting streptomyces aureofaciens freezing shake flask hypha to replace spore. The method comprises: (1) spore culture, (2) shake flask hypha culture, (3) seed culture, and (4) deep liquid ventilation fermentation. According to the present invention, a new inoculation process (shake flask hypha fermentation) is emphatically developed, the freezing shake flask hypha is adopted to replace the spore to be inoculated so as to reduce a use amount of the spore, improve consistency of a fermentation seed age and seed quality, stabilize microbial metabolism, reduce seed preparation workload and labor intensity, and improve resistance production capacity and work efficiency of streptomyces aureofaciens.

The present invention relates to a method for increasing the fermentation level of tetracycline. The method is characterized in that during a fermentation process of tetracycline production using Streptomyces aureus as a fermentation strain, the carbon source and the nitrogen source of a material supplementation culture medium and other substances are subjected to separated sterilization, and the separated material supplementation is performed according to the requirements of the strain on the carbon source and the nitrogen source. According to the present invention, the carbon source and the nitrogen source in the material supplementation culture medium during the fermentation production process using the Streptomyces aureus as the fermentation strain are respectively subjected to the sterilization treatment, the quality of the material obtained through the method is good, the adverse effect of the toxic substance produced from the sugar and nitrogen mixed sterilization on the bacteria is reduced, the utilization efficiency of the sugar water is increased, and the fermentation level is increased; and during the material supplementation process, the material supplementation amount is flexibly controlled according to the requirements of the strain on the carbon source and the nitrogen source, such that the requirements of the bacterial growth reproduction and synthesis at the growth stage can be completely met, the high degree utilization of the carbon source and the nitrogen source can be ensured, the supplementation amount during the process can be reduced, the fermentation unit can be increased, and the material consumption can be reduced.