Streptomyces aureus fermentation tetracycline production method

A kind of Streptomyces aureus and tetracycline technology, which is applied in the fields of biology and medicine, can solve the problems of the appearance quality of the strain to the influence of the inner spore vitality, the complex composition is difficult to accurately determine, and the appearance quality of the inclined plane fluctuates greatly, etc., and achieves strong adaptability, The effect of improved fermentation level and shorter fermentation cycle

Inactive Publication Date: 2019-02-22
NINGXIA QIYUAN PHARMA
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] (1) Bran is the only carbon source provider for spore germination and growth, and its quality is very unstable due to the production location and processing method of wheat. Although agar is used as a coagulant, it has a special effect on the growth of tetracycline slant spores, and its composition is complex Difficult to determine accurately, so the appearance quality of the inclined surface fluctuates greatly due to its influence
[0004] (2) In recent years, due to industrial development, environm...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Streptomyces aureus fermentation tetracycline production method
  • Streptomyces aureus fermentation tetracycline production method
  • Streptomyces aureus fermentation tetracycline production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 (repeat three groups)

[0019] 1. Preparation of spore medium:

[0020] Spore medium formula: bran 1.0%, dipotassium hydrogen phosphate 0.003%, starch 1.0%, magnesium sulfate 0.01%, corn steep liquor 0.3%, yeast powder 0.03%, calcium carbonate 0.01%.

[0021] Preparation method: Dissolve the above materials (except calcium carbonate) and put them in a fixed container, add calcium carbonate to fully dissolve, stir evenly, control the pH at about 7.2, divide into 500ml triangular flasks, each bottle contains 120ml. Carry out sterilization control sterilization temperature 117-121 ℃, pressure 0.08-0.12MPa, sterilization 20-25min, pH after sterilization is 6.4-6.7.

[0022] 2. Spore culture:

[0023] Take 1 tetracycline original strain (Streptomyces aureus), add 1ml of sterile water, shake well, absorb 0.5ml, insert it into the above-mentioned spore culture medium under aseptic conditions, shake well, and culture at 33-35°C for 17 -25 hours.

[0024] 3. Sha...

Embodiment 2

[0044] Embodiment 2 (repeat three groups)

[0045] 1. Preparation of spore medium:

[0046] Spore medium formula: bran 1.2%, dipotassium hydrogen phosphate 0.005%, starch 1.2%, magnesium sulfate 0.02%, corn steep liquor 0.5%, yeast powder 0.05%, calcium carbonate 0.02%.

[0047] Preparation method: Dissolve the above materials (except calcium carbonate) and put them in a fixed container, add calcium carbonate to fully dissolve, stir evenly, control the pH at about 7.2, divide into 500ml triangular flasks, each bottle contains 120ml. Carry out sterilization control sterilization temperature 117-121 ℃, pressure 0.08-0.12MPa, sterilization 20-25min, pH after sterilization is 6.4-6.7.

[0048] 2. Spore preparation:

[0049] Take 1 tetracycline original strain (Streptomyces aureus), add 1ml of sterile water, shake well, absorb 0.5ml, insert it into the above-mentioned spore culture medium under aseptic conditions, shake well, and culture at 33-35°C for 17 -25 hours.

[0050] 3....

Embodiment 3

[0070] Embodiment 3 (repeat three groups)

[0071] 1. Preparation of spore medium:

[0072] Spore medium formula: bran 1.5%, dipotassium hydrogen phosphate 0.006%, starch 1.6%, magnesium sulfate 0.03%, corn steep liquor 0.8%, yeast powder 0.06%, calcium carbonate 0.03%.

[0073] Preparation method: Dissolve the above materials (except calcium carbonate) and put them in a fixed container, add calcium carbonate to fully dissolve, stir evenly, control the pH at about 7.2, divide into 500ml triangular flasks, each bottle contains 120ml. Carry out sterilization control sterilization temperature 117-121 ℃, pressure 0.08-0.12MPa, sterilization 20-25min, pH after sterilization is 6.4-6.7.

[0074] 2. Spore culture:

[0075] Take 1 tetracycline original strain (Streptomyces aureus), add 1ml of sterile water, shake well, absorb 0.5ml, insert it into the above-mentioned spore culture medium under aseptic conditions, shake well, and culture at 33-35°C for 17 -25 hours.

[0076] 3. Sha...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a streptomyces aureus fermentation tetracycline production method. The streptomyces aureus fermentation tetracycline production method comprises the technical processes of dissolving and diluting original streptomyces aureus strains, inoculating and uniformly shaking the dissolved original streptomyces aureus strains into a spore culture medium for cultivation at 33-35 DEGC for 17-25 hours, and sequentially performing seed cultivation and fermented cultivation, wherein the spore culture medium is composed of 1.0-1.5% of bran, 0.003-0.006% of dipotassium phosphate, 1.0-1.6% of starch, 0.01-0.03% of magnesium sulfate, 0.3-0.8% of corn pulp, 0.03-0.06% of yeast powder and 0.01-0.03% of calcium carbonate. Compared with a slant culture manner in the prior art, the streptomyces aureus fermentation tetracycline production method performs fermented tetracycline production through a spore cultivation medium, and by culturing spores through the culture medium, overcomesthe influence factors of agar in a slant culture medium, thereby having the advantages of being free from influence of raw materials, easy in material acquisition and stable in quality; shake-flake seeds are inoculated into the strains cultured through spore solution to ensure robust and well-spread mycelium, and the cultured strains are high in adaptability to culture solutions of every grade, high in metabolism velocity when moved into a fermentation tank, early in tetracycline secretion, short in fermentation cycle and capable of greatly increasing the fermentation level.

Description

technical field [0001] The invention belongs to the technical field of biology and medicine, and in particular relates to a method for fermenting and producing tetracycline by Streptomyces aureus. Background technique [0002] Tetracycline is a class of macrolide antibiotics, mainly produced by Streptomyces aureus. The traditional fermentation strain is to prepare the slant spores from the frozen tube strain, and then inoculate the seed medium from the spores, and inoculate the fermentation medium from the well-grown seeds aseptically. Among them, the slant medium uses bran as the main carbon source and trace element supply, agar as the coagulant, and inorganic phosphate to provide phosphorus and a small amount of nitrogen source. There are following defects in the bacterial classification prepared by above-mentioned culture medium formula: [0003] (1) Bran is the only carbon source provider for spore germination and growth, and its quality is very unstable due to the pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P29/00C12R1/485
CPCC12P29/00
Inventor 章惠霞李学兵张志陈惠琴
Owner NINGXIA QIYUAN PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap