Streptomyces aureus fermentation tetracycline production method

Abstract

The invention relates to a streptomyces aureus fermentation tetracycline production method. The streptomyces aureus fermentation tetracycline production method comprises the technical processes of dissolving and diluting original streptomyces aureus strains, inoculating and uniformly shaking the dissolved original streptomyces aureus strains into a spore culture medium for cultivation at 33-35 DEGC for 17-25 hours, and sequentially performing seed cultivation and fermented cultivation, wherein the spore culture medium is composed of 1.0-1.5% of bran, 0.003-0.006% of dipotassium phosphate, 1.0-1.6% of starch, 0.01-0.03% of magnesium sulfate, 0.3-0.8% of corn pulp, 0.03-0.06% of yeast powder and 0.01-0.03% of calcium carbonate. Compared with a slant culture manner in the prior art, the streptomyces aureus fermentation tetracycline production method performs fermented tetracycline production through a spore cultivation medium, and by culturing spores through the culture medium, overcomesthe influence factors of agar in a slant culture medium, thereby having the advantages of being free from influence of raw materials, easy in material acquisition and stable in quality; shake-flake seeds are inoculated into the strains cultured through spore solution to ensure robust and well-spread mycelium, and the cultured strains are high in adaptability to culture solutions of every grade, high in metabolism velocity when moved into a fermentation tank, early in tetracycline secretion, short in fermentation cycle and capable of greatly increasing the fermentation level.

Description

technical field [0001] The invention belongs to the technical field of biology and medicine, and in particular relates to a method for fermenting and producing tetracycline by Streptomyces aureus. Background technique [0002] Tetracycline is a class of macrolide antibiotics, mainly produced by Streptomyces aureus. The traditional fermentation strain is to prepare the slant spores from the frozen tube strain, and then inoculate the seed medium from the spores, and inoculate the fermentation medium from the well-grown seeds aseptically. Among them, the slant medium uses bran as the main carbon source and trace element supply, agar as the coagulant, and inorganic phosphate to provide phosphorus and a small amount of nitrogen source. There are following defects in the bacterial classification prepared by above-mentioned culture medium formula: [0003] (1) Bran is the only carbon source provider for spore germination and growth, and its quality is very unstable due to the pro...

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Problems solved by technology

[0003] (1) Bran is the only carbon source provider for spore germination and growth, and its quality is very unstable due to the production location and processing method of wheat. Although agar is used as a coagulant, it has a special effect on the growth of tetracycline slant spores, and its composition is complex Difficult to determine accurately, so the appearance quality of the inclined surface fluctuates greatly due to its influence

[0004] (2) In recent years, due to industrial development, environmental pollution has become increasingly serious, and the quality of bran and agar is affected by growth conditions and environment, which is more and more unstable, resulting in unstable quality of the production strains prepared;

[0005] (3) Changes in the quality of raw materials such as bran lead to changes in the newly selected strains. The above-mentioned formula is not suitable for the production of new strains, and the prepared strains are affected from the appearance quality to the internal spore vitality.

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