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Streptomycete bacterial strains and combined application of Streptomycete bacterial strains for preventing and treating sclerotinia rot of colza

A technology of Streptomyces and Streptomyces fissures, applied in the field of microorganisms, can solve problems such as low efficiency, no application, and many operating steps

Inactive Publication Date: 2015-01-28
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among natural products, the research and development of botanical pesticides are the most concerned. At present, some studies have shown that the active ingredients in plant extracts have a certain inhibitory effect on the plant. However, the extraction of plant active ingredients has many steps and low efficiency, but no application has been seen. Actinomycetes have become the main resource for biological control of plant diseases and insect pests and the main source of biopesticide development because of their easy production of active substances such as anti-pathogenic components
Most of the fungi and bacteria that have been reported to have inhibitory effects in the biological control of rape sclerotinia at home and abroad, such as Trichoderma, Pseudomonas fluorescens and Bacillus, screen the biological agents that antagonize actinomycetes to the pathogen of rape sclerotinia from the soil. There are not many reports on prevention and treatment

Method used

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  • Streptomycete bacterial strains and combined application of Streptomycete bacterial strains for preventing and treating sclerotinia rot of colza

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Embodiment 1: ( Streptomyces rimosus M527 and Streptomyces aureofaciens Isolation and screening of M930)

[0017] Follow these steps:

[0018] (1) Materials and culture medium: The materials used to isolate Streptomyces fissures M527 and Streptomyces aureus M930 are soil samples collected from saline-alkali land in the suburbs of Tai’an City, Shandong Province. Potassium acid potassium PDA medium (the formula is: weigh 200g potatoes, wash, peel and chop them, add 1L of distilled water to boil for half an hour, filter with gauze, add 20g of glucose and 20g of agar, fully dissolve and filter with gauze while hot, Dispense into Erlenmeyer flasks or glass test tubes, sterilize under high pressure at 121°C for 20 minutes; add potassium dichromate 20 μg mL when in use -1 ; In the following experiments, the medium used for routine culture of actinomycetes was all configured with distilled water);

[0019] (2) Separation and purification: put the above-mentioned freshly ...

Embodiment 2

[0027] Embodiment 2: ( Streptomyces rimosus M527 and Streptomyces aureofaciens M930 Preparation method of fermentation metabolites)

[0028] Follow these steps:

[0029] (1) Activation culture of strains: the stored Streptomyces rimosus M527 and Streptomyces aureofaciens M930 spore suspension (1×10 8 cfu mL -1 ) into the potato dextrose liquid medium, cultivated for 48 hours at 28°C±1°C for fermentation;

[0030] (2) Fermentation culture: Fermentation medium contains 10g of soybean cake powder, 4g of corn flour, 20g of soluble starch, 5g of glucose, 5g of peptone, and CoCl 2 0.02g, beef extract 5g, CaCO 3 5g, 5g of yeast extract, 50mL of fermentation medium per 300mL triangular bottle; after preparation, sterilize at 121°C for 20min, and use potato glucose liquid medium to culture Streptomyces rimosus M527 and Streptomyces aureofaciens M930 was inserted into the fermentation medium at a rate of 5% (v / v), and fermented for 84 hours at 28°C±1°C with a shaker ...

Embodiment 3

[0033] Embodiment 3: ( Streptomyces rimosus M527 and Streptomyces aureofaciens Inhibition of M930 metabolites against Sclerotinia sclerotiorum)

[0034] (1) Accurately weigh Streptomyces rimosus M527 and Streptomyces aureofaciens M930 metabolite 0.1g, and add 10mL volume of sterile water, ultrasonic vibration to fully dissolve, respectively prepare the mother solution with a final concentration of 10 g / L;

[0035] (2) Determination of antibacterial activity: Take the above-mentioned mother liquor and dilute it into solutions with a concentration of 1g / L and 0.5g / L respectively, put 1mL of each concentration solution in a sterile petri dish, and rapidly mix ( Streptomyces rimosus M527 and Streptomyces aureofaciens The final concentrations of M930 metabolites are respectively 0.1g / L and 0.05g / L), and after cooling, put a diameter of 4mm Sclerotinia sclerotiorum bacterium cake on each medium plane respectively, and the bacteria cake is connected to the center ...

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Abstract

The invention discloses streptomycete bacterial strains and a combined application of the streptomycete bacterial strains for preventing and treating sclerotinia rot of colza and belongs to the technical field of microorganisms. The actinomycetes are separated from a 10-15cm deep soil sample collected in saline-alkali soil in suburb of Taian City, Shandong Province by using an actinomycetes separating technique. Authenticated by microbial taxonomy, the strains are named as streptomyces rimosus M527 and streptomyces aureofaciens M930, wherein S.rimosus M527 is preserved in China Center For Type Culture Collection on June 19th, 2013 with the preservation number of CCTCCNO: M2013270; S.aureofaciensM930 is preserved in the CCTCC on December 25th, 2013 with the preservation number of CCTCCNO:M2013711. The two bacterial strains can ferment to generate active substances which have relatively strong antagonism to sclerotinia sclerotiorum(Lib.)debary, and can be used for preventing and treating sclerotinia rot of colza. When the fermenting products of the two bacterial strains are mixed in a weight ratio of 4 to 1 to prepare a mixed preparation, the effect for preventing sclerotinia rot of colza is more remarkable than that of the two metabolites which are respectively used as single preparations.

Description

technical field [0001] The invention relates to the technical field of microbes, and relates to the application of metabolites of two strains of biocontrol actinomycetes, Streptomyces fissures M527 and Streptomyces aureus M930, in the prevention and treatment of rape sclerotinia. technical background [0002] Rapeseed, native to my country, is planted both in the north and the south. Rapeseed is a low-fat vegetable and contains dietary fiber, so it has high nutritional value and medicinal effect. Sclerotinia sclerotiorum (Lib.) de Bary) caused by Sclerotinia sclerotiorum (Lib.) de Bary is a worldwide disease and ranks first among the three major diseases of rapeseed in my country. Drying, reduced siliques, shriveled seeds, reduced thousand-grain weight and oil yield, and reduced yield are one of the main factors affecting the yield and quality of rapeseed. The disease is particularly serious in the main rape producing areas in the middle and lower reaches of the Yangtze Riv...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/59C12R1/485
CPCA01N63/00C12N1/20C12N1/205C12R2001/49C12R2001/59
Inventor 马正俞晓平路丹丹罗帅边亚琳王正亮申屠旭萍
Owner CHINA JILIANG UNIV
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