The invention relates to microbe technique field, endophytic fungus of Chinese Holly of the invention collected from Chinese Holly living plant of Tianmu Mountain of Linan City is obtained by endophytic fungus microbe separation technology, named as Trichoderma harzianum Rifai stem L identified by microbial taxonomy stored by general microbilogy centre of China Forestry Culture Collection Center on date of august 14th 2006, registration number is CGMCC NO.1780. The most prominent feature is the stem can generate activated component seaquiterpenoids Trichothec-9-en-4-ol,12,13-epoxy-,acetate,(4beta)-(8CI,9CI) by fermentation for inhibiting phytopathogenic fungi bacteria, it is an important microbe resource in agriculture.