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Ginseng endophytic fungus and application thereof

A technology of endophytic fungi and ginseng, which is applied in the field of microorganisms to achieve the effects of increasing the content of ginsenoside Rc and increasing yield

Active Publication Date: 2016-03-23
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, there is no literature report on the isolation of Schizophyllum vulgaris from Panax ginseng

Method used

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  • Ginseng endophytic fungus and application thereof
  • Ginseng endophytic fungus and application thereof
  • Ginseng endophytic fungus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Isolation and purification of ginseng endophytic fungi

[0051] The ginseng endophytic fungus of the present invention is a bacterial strain obtained from cultivated ginseng roots in Tonghua City, Jilin Province.

[0052] The ginseng endophytic fungus of the present invention is separated and obtained by the following steps: washing with tap water for 30 minutes to remove sediment, and then washing with deionized water for 3 times. Cut the roots into 2cm-long root segments and follow the procedure for surface disinfection: 75% ethanol, 30s→1.3M sodium hypochlorite (3-5% available chlorine), 3min→75% ethanol, 30s→sterile deionized water wash 3 times . After the filter paper was blotted to dry the residual water, the root segment was cut into 5mm thick with a sterilized scalpel, and placed in PDA (potato 200g / L, glucose 20g / L, agar, 15g / L) containing 100mg / L penicillin for cultivation Incubate at 26±2°C on the substrate. The samples were observed for fungal ...

Embodiment 2

[0055] Embodiment 2: Ginseng hairy root suspension culture characteristics

[0056] What the present invention adopts is suspension culture, and 1 / 2 MS culture solution 100ml is contained in the Erlenmeyer flask of 250ml volume, the ginseng hairy root that the pre-liquid cultivation is good is inoculated in the culture medium that has been sterilized, and each bottle is inoculated 1g, at 25 ℃ , 20-50 days of dark cultivation at 135rpm, and the culture medium was replaced once a week.

[0057] like figure 2 As shown, the ginseng hairy roots of the present invention grow in clusters in 1 / 2MS liquid medium, wherein A is the overall shape of the hairy root, and B is the side shape of the hairy root.

Embodiment 3

[0058] Embodiment 3: Determination of content of saponins in ginseng hairy root

[0059] When the ginseng hairy roots prepared in Example 2 are harvested, the culture medium is discarded, washed with distilled water for 3 times, and the residual water is blotted with filter paper. After drying at 50°C to constant weight, the content of ginsenosides was determined according to the following method.

[0060] High-performance liquid chromatography (HPLC) was used to analyze the content of saponins in hairy roots of ginseng, mainly to quantitatively detect the contents of Rc, Rb1, Rb2, Re, Rd, Rg1, Rg2, Rg3 and Rh2. Specific steps are as follows:

[0061] Preparation of ginseng hairy root HPLC injection sample: Accurately weigh the hairy root dried to constant weight, record the dry weight and grind it into powder. Accurately weigh 0.5 g of hairy root powder, add 250 ml of ethanol for ultrasonic extraction for 60 minutes, and concentrate to 10 ml by rotary evaporation. The obta...

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Abstract

The invention discloses a ginseng endophytic fungus. The ginseng endophytic fungus is separated from a living plant body of Araliaceae Panax species Panax ginseng C.A. Mey. with an endophytic fungus separation and purification technology and is identified as Schizophyllum commune according to microbial taxonomy. The strain is collected in the CGMCC (China General Microbiological Culture Collection Center) (Institute of Microbiology, Chinese Academy of Sciences), and the collection number is CGMCC 11009. The invention further discloses an application of the ginseng endophytic fungus to increase of the biomass of tissue culture ginseng hairy roots of host ginseng and the content of ginsenosides in the host ginseng.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to ginseng endophytic fungi and applications thereof. Background technique [0002] Ginseng (PanaxginsengC.A.Mey.) is a plant of the genus Panax genus Araliaceae. Its dried roots and rhizomes are used as medicine with the same name. It was first recorded in "Shen Nong's Herbal Classic" and listed as top grade. It is a commonly used traditional Chinese medicine in my country. Sweet, slightly bitter, slightly warm in nature. Return lung, spleen, heart, kidney meridian, have the effects such as invigorating vitality, invigorating spleen and benefiting lung, promoting body fluid to quench thirst, calming the nerves and improving intelligence. In modern clinical practice, it is mainly used to treat heart-qi deficiency syndrome, coronary heart disease, arrhythmia and other diseases. It is one of the most widely used and most deeply researched traditional Chinese medicines. Modern stud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 韩婷陈泠秦路平辛海量郑承剑贾敏翟欣沈慧杨阳
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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