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Streptomycete strains and combined application thereof in prevention and treatment of pepper seedling blight

A technology of Streptomyces albicans and Streptomyces albicans, applied in the field of microorganisms, can solve problems such as rare biological control reports

Active Publication Date: 2015-01-21
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that most of the fungi and bacteria that have inhibitory effect on biological control of pepper blight are fungi and bacteria, such as Trichoderma harzianum and Serratia, and the biological control reports of screening antagonistic actinomycetes from soil to the pepper blight pathogenic fungus are still unknown. rare

Method used

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  • Streptomycete strains and combined application thereof in prevention and treatment of pepper seedling blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: ( Streptomyces albus 2013 and Streptomyces fradiae Isolation and screening of M931)

[0018] Follow these steps:

[0019] (1) Materials and culture medium: The materials used to isolate Streptomyces albicans 2013 and Streptomyces flexneri M931 were soil samples collected from the rhizosphere of plants in Xixi Wetland, Zhejiang, and the medium used for the isolation of actinomycetes was dichromium-containing Potassium acid potassium PDA medium (the formula is: weigh 200g potatoes, wash, peel and chop them, add 1L of distilled water to boil for half an hour, filter with gauze, add 20g of glucose and 20g of agar, fully dissolve and filter with gauze while hot, Dispense into Erlenmeyer flasks or glass test tubes, sterilize under high pressure at 121°C for 20 minutes; add potassium dichromate 20 μg mL when in use -1 ; In the following experiments, the medium used for routine culture of actinomycetes was all configured with distilled water);

[0020] (2) ...

Embodiment 2

[0028] Embodiment 2: ( Streptomyces albus 2013 and Streptomyces fradiae Preparation method of M931 fermentation metabolite)

[0029] Follow these steps:

[0030] (1) Activation culture of strains: the stored Streptomyces albus 2013 and Streptomyces fradiae M931 spore suspension (1×10 8 cfu mL -1 ) into the potato dextrose liquid medium, cultivated for 48 hours at 28°C±1°C for fermentation;

[0031] (2) Fermentation culture: Fermentation medium contains 10g of soybean cake powder, 4g of corn flour, 20g of soluble starch, 5g of glucose, 5g of peptone, and CoCl2 0.02g, beef extract 5g, CaCO 3 5g, 5g of yeast extract, 50mL of fermentation medium per 300mL triangular bottle; after preparation, sterilize at 121°C for 20min, and use potato glucose liquid medium to culture Streptomyces albus 2013 and Streptomyces fradiae M931 was respectively inserted into the fermentation medium at 5% (v / v), and fermented for 84 hours at 28°C ± 1°C with a shaker speed of 180 r / min...

Embodiment 3

[0034] Embodiment 3: ( Streptomyces albus 2013 and Streptomyces fradiae Inhibitory effect of M931 metabolites on Pepper solanum blight)

[0035] (1) Accurately weigh Streptomyces albus 2013 and Streptomyces fradiae M931 metabolite 0.1g, add 10mL volume of sterile water, fully dissolve by ultrasonic vibration, respectively prepare the mother solution with a final concentration of 10 g / L;

[0036] (2) Determination of antibacterial activity: Take the above-mentioned mother liquor and dilute it into solutions with a concentration of 1g / L and 0.5g / L respectively, put 1mL of each concentration solution in a sterile petri dish, and rapidly mix ( Streptomyces albus 2013 and Streptomyces fradiae M931 metabolite final concentration is respectively 0.1g / L and 0.05g / L), after cooling, put 1 diameter on each culture medium plane respectively and be the capsicum solani bacterium bacterium cake of 4mm, bacterium cake is connected in the culture dish center ( The diamete...

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Abstract

The invention discloses streptomycete strains and a combined application thereof in prevention and treatment of pepper seedling blight, and relates to the technical field of microorganisms. The streptomycetes disclosed by the invention are separated and obtained from a deep soil sample of 10-15cm collected from the Hangzhou xixi wet land in Zhejiang by adopting a streptomycete separation technology, are identified by microbial taxonomy, are named streptomyces albus (streptomyces albus) 2013 and streptomyces fradiae (streptomyces fradiae) M931,S.albus2013; the streptomyces albus 2013 is preserved at the China Center for Type Culture Collection on June 19, 2013; the preservation number is CCTCC NO:M2013269; the S.fradiae M931 is preserved at the China Center for Type Culture Collection on December 25, 2013; and the preservation number is CCTCC NO:M2013712. The two bacterial strains can be fermented to generate active substances with relatively strong antagonistic effects on rhizoctonia solani kuhn (Rhizoctonia solani Kuhn), and can be used for preventing and treating plant diseases such as the pepper seedling blight; when fermentation products of the two bacterial strains are mixed to prepare the mixed preparation according to the mass percent of 3 to 2, the effect for preventing and treating the pepper seedling blight is more significant in comparison with the effect when two metabolites are respectively used as single preparations.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and relates to the application of metabolites of two biocontrol actinomycetes, Streptomyces albicans 2013 and Streptomyces flexneri M931, in the prevention and treatment of pepper blight. technical background [0002] Pepper (Capsicum annuum L.) is one of the favorite vegetables and condiments, and plays an important role in vegetable production. Pepper blight caused by Rhizoctonia solani is an important disease that causes dead seedlings and fallen seedlings in the early stage of pepper seedling cultivation. It mostly occurs at the cotyledon stage of peppers, causing the stem base of the seedlings to rot and constrict and die when they are close to the ground. , When the soil humidity in the seedbed is high, the disease develops rapidly, which can cause a large number of seedlings to die. In recent years, the occurrence of pepper blight has increased year by year, posing a serious threat ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P1/00C12P1/06C12R1/47C12R1/54
CPCA01N63/00C12P1/06C12N1/205C12R2001/47C12R2001/54A01N2300/00
Inventor 马正俞晓平罗帅路丹丹边亚琳许益鹏王正亮申屠旭萍
Owner CHINA JILIANG UNIV
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