51 results about "Streptomyces fradiae" patented technology

The invention discloses a novel strain CLIMAX-VSF of streptomyces fradiae. The preservation number of the novel strain is CGMCC No. 5133; the preservation date is August 12, 2011; and the preservation department is China General Microbiological Culture Collection Center. The invention further discloses a microbial inoculum containing the novel strain. The novel strain CLIMAX-VSF of the streptomyces fradiae, which is provided by the invention, has quite obvious performances of disease resistance, nitrogen fixation, phosphate solubilization and somatotropin secretion. By using the strain, the germination rate and the growth speed in a seedling stage can be obviously improved under the condition that nutrients are not added to soil, and furthermore, diseases in the seedling stage and a growth period are obviously inhibited. On the aspect that the strain is used for the mixed preparation and the synergy of a fertilizer, the strain shows to have the favorable effect of activating soil nutrients and the performance of resisting a plurality of cultivation obstacles.

The invention pertains to the technical field of biology and medicine, and relates to a bacterial strain of streptomyces fradiae NTGA-334 with a preseravation no. of CGMCC No.2074, and a process for using the bacterial strain to ferment and transform Compound K in glycol group in various notoginsenosides so as to prepare 20-O-beta-D-glucopyranoside-20 (S)-protopanaxadiol. The culture media are Gause's no.1, ISP2, corroding acid (HSG culture medium) and asparagine culture medium. During the fermentation, the dosing proportions of various notoginsenosides are 0.1-10% (w / v), fermenting temperature is 18-50 DEG C, dynamically adjusted pH value of 0.1-35%(w / v) aqueous ammonia is 0.1-30% (w / v). The invention transforms and ferments in a scaled way various notoginsenosides, prepares Compound K, provides a streptomycete stain that is of steady condition, simple nutrition requirement and good growth, and provides a process for fermenting and separating by a simple and easy fully automatic fermenting tank. Using this bacterial strain and process to produce Compound K, the production cost is low, the foreign matter is less, and the yield rate is high.

The invention discloses an organic material decomposing agent, which comprises the following components in percentage by weight: 10-50 parts of Streptomyces fradiae CGMCC No.5133 microbial inoculum, 5-20 parts of Myceliophthora thermophila CGMCC No. 5134 microbial inoculum, 0-20 parts of Trichoderma viride microbial inoculum, 0-20 parts of Bacillus subtilis microbial inoculum and 0-20 parts of microzyme inoculum. The invention provides the organic material decomposing agent with auxin accumulation function which utilizes the quick accumulation of auxin generated by the Streptomyces fradiae in the medium temperature stage and the matching of high temperature function of the Myceliophthora thermophila in the anaphase to realize quick treatment and final resource utilization of organic materials, and treated organic solid wastes are fundamentally transformed, embody good performances in improving soil, promoting growth and inhibiting soil-borne diseases and meanwhile are environment-friendly and sanitary.

The invention relates to a production method capable of increasing yield of neomycin. According to the production method, one or several of additives which are lysine, ferrous lactate, tyrosine, methionine, hydrolysis casein, vitamin B12, fructose, glucosamine, folic acid, riboflavin and tryptophan are added to a conventional fermentation medium comprising peanut meal, corn starch, rice flour, yeast powder, glucose, peptone, ammonium sulfate, sodium chloride, light calcium carbonate and KH2PO4-containign streptomyces fradiae. The production method has the advantages that the yield of the neomycin is increased by above 5-20%, the cost is reduced, and the fermentation efficiency is high. The neomycin is used for treating human diseases, widely used in animal husbandries and used as antibiotics.

The invention relates to a method for improving the percent conversion of a tylosin component A. With the method, streptomyces fradiae is used as a producing strain and is fermented to produce the tylosin by first-level seed tank fermentation culture, second-level seed tank fermentation culture and fermentation tank fermentation culture. The method is characterized in that nonionic surfactant of which the mass concentration is 0.5-2.5% is added into a fermentation culture medium after the fermentation tank fermentation culture enters a stable period after a logarithmic growth phase is finished. The nonionic surfactant is added into the culture medium during a fermentation culture period to the stable period so as to enhance the permeability of a mycelium cell, so that a metabolin in a cell is accelerated to quickly release out of the cell to relieve the feedback inhibition of macrosin methyltransferase and improve the enzyme activity, and finally, a tylosin component C is accelerated to quickly covert into the tylosin component A. The tylosin component A in the current industrial production generally accounts for 80-85%, and the content of the tylosin component A can finally achieve 90-94% according to the method disclosed by the invention.

The invention relates to a culture medium for producing tylosin by fermenting streptomyces fradiae, and a fermenting method. A shaking bottle seed culture medium, a first-level seed culture medium, a second-level seed culture medium and a fermentation medium all comprise beer yeast dry residue and earthworm powder. According to the invention, the formula of the culture medium is optimized, so that the problem about the costs of raw materials and auxiliary materials is solved, the influence on the sources of the raw materials and auxiliary materials is reduced to the maximum degree, sufficient supply of the raw materials and auxiliary materials is guaranteed, and stable and high-efficiency production of the tylosin is realized. By adopting the culture medium the fermentation unit can be increased, the fermentation period can be shortened, the inoculation amount of primary seeds is reduced, and the quantity of the component A of the tylosin is increased.

The invention provides a compacted soil improvement method, which comprises the following steps: the first step, turning over the compacted soil with a shallow tiller and breaking the soil block with a rotary tiller; The soil improver, and then use the deep tiller to bury the soil improver into the soil; the third step, apply the organic compound fertilizer on the soil surface and use the shallow tiller to bury the organic compound fertilizer into the soil; the fourth step, in the soil Sprinkle the soil conditioner on the surface and cover with 2-3 cm crushed soil, then apply organic compound fertilizer, and finally cover with a layer of 3-5 cm crushed soil. The compacted soil improvement method has fewer steps, less workload and is easy to operate. Brevibacillus lateralosporus and Streptomyces freundii in the soil conditioner used in the compacted soil can multiply rapidly in compacted soil, combined with potassium fulvic acid, can improve Soil aggregate structure, loose soil, improve soil water and fertilizer retention capacity, so as to quickly improve the growth of crops in compacted soil.

The invention discloses a biological organic pesticide and fertilizer, which comprises the following raw materials in part by weight: 100 parts of fermented organic fertilizer and 0.2 to 2 parts of streptomyces fradiae fungicide. The invention further provides a preparation method for a biological organic pesticide and fertilizer, which comprises the following steps of: A10, adding an organic material composting agent into the organic fertilizer material and fermenting for 6 to 12 days; A20, when the temperature is dropped to below 40 DEG C and the water content is less than 35 percent, adding the streptomyces fradiae fungicide, and fermenting for 3 to 6 days, wherein the weight part ratio of the fermented organic fertilizer to the streptomyces fradiae fungicide is 100:0.2-2; A30, when the temperature is dropped to below 35 DEG C and the water content is less than 30 percent, grinding and screening. According to the novel idea of 'killing bacterium by bacterium, killing insect by bacterium, and combining fertilizer with pesticide', special microorganism bacterium with no toxicity and damage to human and beast and safe performance is applied to the preparation process of biological organic fertilizer to produce the biological organic pesticide and fertilizer with the characteristics of biological pesticide and biological fertilizer.

The invention provides a streptomyces fradiae solid culture medium, a culture method and a preparation method of an agricultural microbial agent, belonging to the field of microbial fermentation. The streptomyces fradiae solid culture medium is prepared from the following components in parts by weight: 3-10 parts of corn flour, 3-18 parts of soybean meal, 18-45 parts of straw powder, 10-30 parts of turfy soil, 20-50 parts of phosphate tailing, 1-10 parts of calcium carbonate and 40-50 parts of nutrient solution. According to the invention, a solid fermentation culture medium is prepared through liquid-solid two-phase fermentation by adopting the solid culture medium and is used for producing an agricultural streptomyces fradiae agent. The fermentation culture medium for streptomyces fradiae spore production, provided by the invention, is suitable for the growth of streptomyces fradiae, and over 7.5*10<9> spores can be produced from each gram of fermentation raw material; and the germination rate of the prepared spore powder can reach 95%, the prepared agricultural microbial agent conforms to the national standard of agricultural microbial agent, and an obvious promotion effect is realized on the crop growth.

The invention relates to a liquid state composite biological multivariable control paddy herbicide. The herbicide is characterized by being prepared from medicinal plants and multiple species inoculant. The medicinal plants comprise, by weight, 5-25 parts of radix euphorbiae lantu, 5-25 parts of cumin, 5-25 parts of garlic, 5-25 parts of radix angelicae pubescentis and 5-25 parts of common cnidium fruits. The multiple species inoculant comprises, by weight, 5-20 parts of bacterial liquid of streptomyces lavendulae, 5-20 parts of bacterial liquid of streptomyces fradiae, 5-20 parts of bacterial liquid of streptomyces viridocbromogenes, 5-20 parts of bacterial liquid of streptomyces griseolus, 5-20 parts of bacterial liquid of alternaria, 5-20 parts of bacterial liquid of botryis cinerea and 5-20 parts of bacterial liquid of curvuloria lunata. The biological multivariable control paddy herbicide is efficient, low in toxicity, environmentally friendly and not likely to produce resistance and particularly has the remarkable restraining effect on barnyard grass, nutgrass flatsedge, semen euphorbiae and duckweed.

The invention belongs to the technical field of microbial fertilizers, and particularly relates to a microbial foliage synergist; the preparation process flow of the invention is as follows: (1) the preparation of a bacillus laterosporus laubach WY9701 preparation; (2) the preparation of a streptomyces fradiae BD04002 metabolite; (3) the preparation of a microbial foliage synergist. The preparation process of the invention adopts an advanced continuous fermentation process of 'liquid submerged fermentation + closed solid fermentation', and uses a fully-closed self-controlled solid fermentation apparatus; the whole process of the solid fermentation is under fully-closed automatic monitoring; environment conditions such as the medium component, the formula, the temperature, the humidity, the ventilation, the pH value and the like are automatically detected, electronically displayed, and automatically controlled; not only the yield is greatly increased, but also the quality of the microbial foliage synergist is guaranteed.

The invention belongs to the technical field of agriculture microbe, and especially relates to an insecticidal biological composite inocula and a preparation method thereof. The insecticidal biological composite inocula comprises composite bacteria, a bacterial strain protective agent, and a carrier; and the composite bacteria is bacillus thuringiensis, paecilomyces lilacinus, beauveria bassiana,pesudomonas fluorescence, streptomyces fradiae, bacillus mucilaginosus, bacillus licheniformis and hygrospinocus var.beigingenis. The insecticidal biological composite inocula has the advantages of wide insecticidal scope, less amount, and low cost, can effectively inhibit harmful flora and insect breeding, can increase the pest-resistant capability of plants, can enhance the soil activity, stressresistance and soil fertility, increases the fertilizer absorption utilization rate, keeps high insecticidal rate, can keeps high survival rate of effective bacterial strains, and prolongs the viability time of the effective bacterial strains.

The invention relates to a culture medium for producing neomycin by utilizing fermentation of streptomyces fradiae. The culture medium comprises a seed culture medium and a fermentation culture medium, wherein soy peptone, biological fish meal, millet meal, zinc lactate, ammonium molybdate and other substances are added into the culture medium, so that metabolism of strains can be benefited, fermentation starting titer can be improved, the aging speed of hyphae can be delayed, the neomycin production capability can be improved, the fermentation cycle can be shortened, the fermentation cost can be reduced, and high-efficiency production of neomycin can be realized.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a composite microbial agent used for soil amelioration and a preparation method of the composite microbial agent. The composite microbial agent used for the soil amelioration includes acidithiobacillus ferrooxidans, pseudomonas fluorescens, streptomyces fradiae, bacillus mucilaginosus, paecilomyces lilacinus, paracoccus denitrificans and bacillus licheniformis. The composite microbial agent provided by the invention can quickly form a PGRR protective layer on the soil surface, reduce harmful microbial content, repair soil biological-ecological balance, enhance soil activity, stress resistance and soil fertility, decompose and activate large, medium and micro nutrient elements insolublein water in soil, improve an absorption and utilization rate of chemical fertilizers, greatly improve fertilizer efficiency, and reduce use of pesticides.

The invention relates to a compound menstruum for extracting tylosin and an extraction method. According to the invention, the compound menstruum formed by mixing butyl acetate, isobutyl acetate and butanol according to the volume ratio of 6:3 to 3.2 to 0.8-1 is used for extracting tylosin in fermentation filtrate through a secondary counter-current extraction method. The method has the advantages of low consumption of organic menstruum, low loss of tylosin, short extraction time, high extraction yield and the like.

The invention provides a clean fermentation process of neomycin, which uses Streptomyces fradiae as producing stain and modifies the composition of sugar supplementing solution, the supplementing rate control and other parameters during fermentation process based on the conventional neomycin microbial fermentation production process. The inventive process is scientific and reasonable, and can improve the concentration of the fermentation product and reduce residual sugar content. The starch consumption of unit neomycin production is remarkably reduced, and the emitted COD pollution load is reduced by approximate 50%, so as to achieve clean production purpose.

A Streptomyces fradiae NTGA-344 strain with the preservation number of CGMCC No.2074 and a method for preparing 20(S)-ginsenoside Rh1 (6-O-belta-D-Glucopyranoside-20(S)-Protopanaxatriol by using the strain to ferment and convert panaxatriol saponins in various notoginsenosides in a fermentation cylinder are provided. 1(GA), ISP2, humic acid (HSG culture medium) and asparagines culture medium are selected as the culture media. In the process of fermentation, the inventory rating proportion of various notoginsenosides is 0.1% to 10 % (w / v), the fermentation temperature is 18 to 50 DEG C, the pH value of 0.1% to 35% (w / v) ammonia is dynamically adjusted to be between 2 and 8, the ventilation ratio is 0.1 to 10 (v / v), the stir speed is 10 to 900r / min, the fermentation time is 5h to 200h, and the macroporous absorbent resin is 0.1% to 30% (w / v). The invention provides the streptomyces yunnanensis with stable property, simple nutritional requirement and good growth and a practical and convenient fermentation cylinder fermentation and separation extraction process for large-scale fermentation and conversion of various notoginsenoside and preparation of 20(S)-ginsenoside Rh1. The 20(S)-ginsenoside Rh1 is prepared by the strain and the technology, thereby having low manufacturing cost, less impurity and high product yield.

The invention relates to a tylosin gene engineering strain. The construction method for the tylosin gene engineering strain comprises the following steps: cloning tylosin through polymerase chain reaction (PCR) to synthesize crucial rate-limiting enzyme gene TylF and Vitreoscilla hemoglobin VHb gene, constructing a recombinant plasmid by using an E. coli-streptomyces shuttle vector, importing the recombinant plasmid into Escherichia coli ET12567 / pUZ8002, performing conjugal transfer culture on the Escherichia coli ET12567 / pUZ8002 and streptomyces fradiae spores, converting streptomyces, screening a converter through apramycin resistance, further confirming the converter by using PCR, and finally obtaining the gene engineering strain. The tylosin gene engineering strain can be directly used in the industrial production of tylosin; and compared with the tylosin gene engineering strain used in the conventional industrial production, the tylosin gene engineering strain has the advantages that the yield of the tylosin can be obviously improved to be 17,000-18,000u / ml and the content of a component A in the tylosin is effectively improved to be 94-95 percent.

The invention relates to the field of microorganisms, in particular to multifunctional streptomyces fradiae and application of the multifunctional streptomyces fradiae as a solid microbial agent. The collection number of the streptomyces fradiae G-1 is CCTCC M 2020250. While preventing and treating soil-borne diseases, the strain can generate active substances such as IAA, cellulase and protease to promote plant growth and soil nutrient utilization, and can degrade phenolic acid plant autotoxic substances to relieve obstacles to continuous cropping of soil. The preparation of the microbial agent adopts a preferable formula and is supplemented with a fermentation process conforming to the growth of strains to obtain the high-activity multifunctional microbial agent. The microbial agent can be combined with straw manuring to promote straw decomposition and can be combined with a commercially available organic fertilizer or farmyard manure to serve as base fertilizer. After application, the ecological balance of the soil is regulated by means of nutrient competition, metabolite regulation and control and the like, the fertility of the soil is enhanced, and the occurrence of soil-borne diseases is reduced. The microbial agent is simple in preparation method, readily available in raw materials, low in production cost, long in shelf life and obvious in disease prevention and yield increase effects.

The invention discloses a biosurfactant composition for soil improvement. The biosurfactant composition is prepared from the following raw materials in parts by weight: 2-20 parts of a composite microbial flora, 20-50 parts of an organic fermentation substance, 15-40 parts of shell powder, 10-30 parts of starch, and 5-30 parts of inorganic salt. The biosurfactant composition for soil improvement, provided by the invention, is prepared by mixing a microbial preparation with the organic fermentation substance, the shell powder, the starch and the inorganic salt, the soil improvement type microbial preparation is obtained through combination of at least two kinds of streptomyces fradiae, photosynthetic bacteria, acidithiobacillus ferrooxidans, bacillus mucilaginosus, bacillus subtilis and bacillus laterosporus, and through a composite of the biological preparation and chemicals, the microenvironment of soil is regulated, so that the growing environment of crops can be improved, and the purpose of increasing the yield per unit area of the crops is realized.

The invention provides an acid soil activity remediation inoculant. The inoculant takes streptomyces fradiae and bacillus licheniformis as active ingredients. Preferably, the streptomyces fradiae CLIMAX-VSF with preservation number as CGMCC No.5133 and the bacillus licheniformis CLIMAX-VBL with preservation number as CGMCC No.12499 are taken as the active ingredients. The invention also provides a preparation method of the inoculant. The product (the inoculant), which consists of high-activity microorganisms, alkaline raw materials (such as sodium silicate and potassium fulvic acid) and high-quality organic matters, can effectively improve and condition acid soil and significantly improve micro-ecological environment of crop rhizosphere soil, so that the microorganisms can be rapidly planted and a dominant flora can be formed; therefore, favorable conditions are created for offering nutrient ingredients to the growth of plants.

The invention discloses a composite protease, a preparation method and application thereof. The invention also discloses a new streptomyces fradiae bacterial strain for manufacturing the composite protease. The collection number of the bacterial strain in the CGMCC (General Microbiology Center of China Committee for Culture Collection of Microorganisms) is CGMCC No.4186. The invention also discloses a method for obtaining the microorganism and the application thereof.

The invention relates to a method for producing sisomicin sulfate by the variation GB-2 of Freund streptomyces and belongs to the technical field of microorganism fermentation. The variation GB-2 is inoculated into a fermentation culture liquid and cultured for 7 day under the temperature of 28 DEG C with a speed of 180r.min<-1>; a permeate liquid equal to or less than 1KDa obtained by carrying out centrifugating and hyperfiltration on a supernatant fluid is pre-treated and then goes on a column of Sephadex LH-20, collects the liquid and detects the antibacterialactivity, then a second apex which is namely the product of sisomicin sulfate is collected after effective liquidoid chromatogram analyzing and HPLC purifying are carried out. Detected by ESI-MS, the molecular weight thereof is m / z 447.7 and is confirmed to be the sisomicin sulfate by a secondary mass spectrum. The method of the invention firstly obtains the sisomicin sulfate in the Freund streptomyces which belongs to the streptomyces of actinomycetes. The sisomicin sulfate is named to be the aminoglycoside antibiotic of the third generation which is the antibiotic with the advantages of the smallest side effect, strongest resistance of pseudomonas aeruginosa, broadest capacity for resisting modifying enzyme and broadest antimicrobial spectrum in the aminoglycoside antibiotics and has a potential market application prospect.

The invention discloses streptomycete strains and a combined application thereof in prevention and treatment of pepper seedling blight, and relates to the technical field of microorganisms. The streptomycetes disclosed by the invention are separated and obtained from a deep soil sample of 10-15cm collected from the Hangzhou xixi wet land in Zhejiang by adopting a streptomycete separation technology, are identified by microbial taxonomy, are named streptomyces albus (streptomyces albus) 2013 and streptomyces fradiae (streptomyces fradiae) M931,S.albus2013; the streptomyces albus 2013 is preserved at the China Center for Type Culture Collection on June 19, 2013; the preservation number is CCTCC NO:M2013269; the S.fradiae M931 is preserved at the China Center for Type Culture Collection on December 25, 2013; and the preservation number is CCTCC NO:M2013712. The two bacterial strains can be fermented to generate active substances with relatively strong antagonistic effects on rhizoctonia solani kuhn (Rhizoctonia solani Kuhn), and can be used for preventing and treating plant diseases such as the pepper seedling blight; when fermentation products of the two bacterial strains are mixed to prepare the mixed preparation according to the mass percent of 3 to 2, the effect for preventing and treating the pepper seedling blight is more significant in comparison with the effect when two metabolites are respectively used as single preparations.

The invention discloses a high throughput screening method for a high-yield tylosin strain based on a flow cytometry, belonging to the technical field of high throughput screening. According to the method, a PI dyeing method is carried out on induced-mutation spores with different activities, active spore regions are correctly distinguished by adjusting and optimizing parameters including FSC, SSC, a voltage threshold and the like, and the interference blended with a background in flow cytometry detection caused by over-small volumes of the spores is eliminated; by carrying out a solid-liquid combined high throughput culture scheme on the separated viable spores, the pore plate growth rate of streptomyces fradiae is effectively increased; and activated streptomyces fradiae is separated based on the flow cytometry, and a novel effective manner is provided for the high throughput screening of the streptomyces fradiae.

The invention relates to a method for rapidly screening high-titer tylosin strain by taking lipase activity as an index and a method for rapidly screening a tylosin medium by taking lipase activity as an index. The method for rapidly screening high-titer tylosin strain by taking lipase activity as the index comprises: adding rhodamine B into a separation plate medium with plant oil as a main carbon source, coating with streptomyces fradiae spore suspension for plate culture, after culture is finished, choosing a single colony with a large ratio of bacterium colony hydrolysis circle diameter to bacterium colony diameter; inoculating a portion of the single colony to an inclined surface, inoculating the other portion of the single colony to a flask-shaking medium, and investigating the tilter. By adding rhodamine B into the medium the hydrolysis circle shows pink color; and according to the ratio of the bacterium colony hydrolysis circle diameter to the bacterium colony diameter, an excellent bacterial strain accord with requirements is chosen, and thus the workload is reduced, the screening efficiency is improved and the probability that a high-yield bacterial strain is chosen is increased. In a similar way, by utilizing lipase activity under different nitrogen-source conditions of streptomyces fradiae as the index, a medium formula suitable for tylosin growth is chosen, and thus the fermentation tilter is improved.

The invention belongs to the technical field of microbial pesticides, and provides a bio-control agent for preventing and controlling root-knot nematodes, and an application thereof in order to solvethe problems of single disease prevention mechanism and low prevention and control effect of existing single strains for preventing and controlling root-knot nematodes. The bio-control agent is prepared by mixing a Bacillus licheniformis-Pseudomonas fluorescens mixed fermentation broth, a streptomyces fradiae fermentation broth and a Phanerochaete chrysosporium-Myrothecium verrucaria mixed fermentation broth according to a volume ratio of (2-5):(1-3):(1-7), and uniformly mixing the obtained mixed solution with quinoa bran according to a mass ratio of 1 kg:(6-8) kg. The above five strains do not antagonize each other and cooperate with each other to kill insects, and the preparation method is simple, so the bio-control agent has a broad application prospect. The bio-control agent can prevent and control root-knot nematodes, promote the growth and development of plants and improve the disease resistance of plants.

The invention belongs to the technical field of fermentation engineering, and particularly relates to streptomyces fradiae SF-4 as well as an application thereof in fermentation of tylosin. The streptomyces fradiae SF-4 was deposited in the China typical culture preservation center on October 9, 2019, with the deposit number of CCTCC NO: M2019791. The invention further discloses a method for the fermentation of the tylosin by the strain, an obtained tylosin fermentation solution can effectively control the synthesis of component D by use of the method, and the quality of the tylosin is improved.

The invention discloses a strain for producing geosmin, and belongs to the technical field of biotechnology. The invention discloses a method for screening and identifying related strains of aroma components produced by microbial metabolism in screened white spirit in the starter propagation process by combining aroma smelling means and using flavor orientation. By using the method, a strain of the geosmin is successfully screened from yeast produced by an aromatic white spirit factory and named as FJ-HX; and according to colony, cellular morphology, physiological and biochemical property and 16SrRNA gene sequence comparison result, the strain is streptomyces fradiae.. The strain can accumulate a large quantity of geosmin in a potato-glucose culture medium. The problem about the source of the geosmin in the aromatic white spirit is solved. The screened streptomyces fradiae can be used as a type strain, and is used for detection and production mechanism research of the strain for producing the geosmin in white spirit production.