Microbial foliage synergist

A technology of microorganisms and synergists, applied in the direction of organic fertilizers, etc., can solve the problems of low utilization, insufficient technological innovation, unstable product quality, etc., and achieve the effect of increasing yield and ensuring stability.

Inactive Publication Date: 2011-07-27
DONGGUAN BAODE BIOLOGICAL ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The development and application of microbial fertilizers in my country began in the 1950s. Due to the lack of research funds, the overall l

Method used

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  • Microbial foliage synergist
  • Microbial foliage synergist

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0084] 1. Preparation of Bacillus lateralis:

[0085] a. Strain selection

[0086] (Ampoule tube) shape and arrangement: the fungus is rod-shaped, with a slightly thinner end, solitary in pairs and piles; the spores are oval, (1-1.2)×(2.5-3) μm, with canoes attached to the side of the spores Shape, spores grow laterally and mesolaterally in spindle-shaped cysts.

[0087] Movement and flagella: able to move.

[0088] b. Bacteria cultivation

[0089] Slant culture: test tube slant medium: beef extract-peptone medium; culture temperature: 32±1°C; culture time: 72-96 hours.

[0090] Shake flask culture: culture temperature: 32±1°C culture time: 30-32 hours

[0091] c. Formulation production

[0092] Seed tank liquid fermentation culture: culture temperature: 32±1°C culture time: 18-20 hours aeration condition 1: 0.3-0.5V / V

[0093] Closed solid fermentation incubator culture:

[0094] Cultivation temperature: 28-35°C Cultivation time: 96-120 hours Cultivation humidity: 60%-...

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Abstract

The invention belongs to the technical field of microbial fertilizers, and particularly relates to a microbial foliage synergist; the preparation process flow of the invention is as follows: (1) the preparation of a bacillus laterosporus laubach WY9701 preparation; (2) the preparation of a streptomyces fradiae BD04002 metabolite; (3) the preparation of a microbial foliage synergist. The preparation process of the invention adopts an advanced continuous fermentation process of 'liquid submerged fermentation + closed solid fermentation', and uses a fully-closed self-controlled solid fermentation apparatus; the whole process of the solid fermentation is under fully-closed automatic monitoring; environment conditions such as the medium component, the formula, the temperature, the humidity, the ventilation, the pH value and the like are automatically detected, electronically displayed, and automatically controlled; not only the yield is greatly increased, but also the quality of the microbial foliage synergist is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers, in particular to microbial foliage synergists. Background technique [0002] The development and application of microbial fertilizers in my country began in the 1950s. Due to the lack of research funds, the overall level of the microbial fertilizer industry has long been low, insufficient technological innovation, unstable product quality, and low utilization. At present, more than 70 countries and regions in the world have developed and applied microbial fertilizers. In the agricultural production of developed countries in Europe and America, the use of microbial fertilizers has reached more than 20% of the total amount of fertilizers, and the total output is increasing at a rate of 10% to 20%. However, the annual microbial fertilizer production in my country only accounts for 0.5% of the chemical fertilizer production. According to experts' estimates, if this ratio reaches 3%, it...

Claims

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Application Information

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IPC IPC(8): C05F11/08
Inventor 张新雄
Owner DONGGUAN BAODE BIOLOGICAL ENG
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