Streptomyces fradiae G-1 and application thereof

A technology of Streptomyces freundii and bacteria agents, applied in the field of microorganisms, can solve problems such as high material costs, increased production costs, and complex processes, and achieve the effects of solving high material costs, restoring ecological balance, and enhancing soil fertility

Active Publication Date: 2021-10-29
辽宁省微生物科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this invention, the function of the Streptomyces flexneri strain is not clear, and the fermentation formula and process are relatively complicated
Another example is CN102329754, titled "a new strain of Streptomyces freundii and its application", the Streptomyces freundii seed liquid is inserted into 60 parts of wheat bran, 5 parts of brown sugar, 30 parts of cottonseed cake, and 1.5 parts of magnesium sulfate. , 1.5 parts of sodium chloride, and 2 parts of calcium carbonate, the original bacterial agent is then inoculated into carriers with different formulations. It is unknown whether the carrier components are suitable for strain survival and long-term storage, and the process is complicated. The cost is high, resulting in the quality of the finished bacterial agent cannot be guaranteed
In addition, in the above two inventions, the material cost of the fermented inoculum alone is 1000-1500 yuan per ton, which undoubtedly increases the production cost, pushes up the price of inoculum, indirectly increases the production burden of farmers, and is not conducive to the development of microbial inoculum. Mass production and wide application

Method used

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  • Streptomyces fradiae G-1 and application thereof
  • Streptomyces fradiae G-1 and application thereof
  • Streptomyces fradiae G-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Isolation, screening and taxonomic identification of strain G-1:

[0054] 1. Isolation and screening of bacterial strains:

[0055] The continuous cropping capsicum plot in Chaoyang County, Liaoning Province was selected, and the capsicum plants were pulled out to collect rhizosphere soil samples by root shaking method. When separating, take 1g soil sample and put it into 99mL sterile physiological saline, vibrate for 30min, and mix 10 -3 、10 -4 、10 -5 100 μL of soil suspension containing 50 μg / mL -1 Potassium dichromate Gaoshi No. 1 solid plate was cultured at 32°C for 5 days, and a single colony of actinomycetes was selected for streaking and purification. The purified strains were inoculated into Gaoshi No. 1 slant medium and stored for later use.

[0056] Pipette 100 μL of pepper root rot pathogen (Fusarium solani) spore suspension and evenly spread it on the Gaoshi No. 1 solid medium, and place a piece of sterile filter paper (d=5mm) on the plate for later use....

Embodiment 2

[0070] Physiological characteristics of strain G-1:

[0071] (1) The strain has antagonistic effects on pathogenic bacteria of various vegetable soil-borne diseases:

[0072] The well-grown pepper root rot pathogen (Fusarium solani), pepper sclerotinia pathogen (Sclerotinia sclerotium), cucumber wilt pathogen F.oxysporium fsp.cucumarinum, tomato early blight pathogen ( Alternaria solani) and Rhizoctonia solani were prepared into 1.0×10 7 cfu / mL pathogen spore suspension, use sterile filter paper pieces (d=5mm) to soak in the spore suspension respectively to become pathogenic bacteria flakes, also make flakes soaked in G-1 strain and put them on Gaoshi No. 1 plate The confrontation culture was carried out, and the control group was cultured with pathogenic bacteria slices alone, and each experiment was repeated 3 times. After culturing at 31°C for 5 days, measure the diameter of the treatment group and the control group, and calculate the antibacterial rate.

[0073] Bacteri...

Embodiment 3

[0102] Preparation of Streptomyces flexneri solid medium:

[0103] In parts by weight, 10-30 parts of rice husk powder, 5-8 parts of bran, 5-8 parts of rice husk, 55.9-79.3 parts of cultivated field soil, 0.3-0.5 part of quicklime, 0.4-0.6 part of ammonium sulfate, Design-expert10.0.3 software was used to carry out D-optimal mixture design, and the optimal formula was determined with the number of viable bacteria as the response value. The results of the medium formula and the number of viable bacteria were shown in Table 6 (see Table 7 for variance analysis). Among them, the particle size of the rice husk powder is 20 mesh, and the cultivated field soil is the daily field soil, which is sieved to make the particle size 10 mesh.

[0104] According to the mixture recorded in Table 6, the materials are fully mixed evenly. In order to make the distribution of quicklime and ammonium sulfate more uniform, put quicklime and ammonium sulfate into water to make a suspension, and mix t...

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Abstract

The invention relates to the field of microorganisms, in particular to multifunctional streptomyces fradiae and application of the multifunctional streptomyces fradiae as a solid microbial agent. The collection number of the streptomyces fradiae G-1 is CCTCC M 2020250. While preventing and treating soil-borne diseases, the strain can generate active substances such as IAA, cellulase and protease to promote plant growth and soil nutrient utilization, and can degrade phenolic acid plant autotoxic substances to relieve obstacles to continuous cropping of soil. The preparation of the microbial agent adopts a preferable formula and is supplemented with a fermentation process conforming to the growth of strains to obtain the high-activity multifunctional microbial agent. The microbial agent can be combined with straw manuring to promote straw decomposition and can be combined with a commercially available organic fertilizer or farmyard manure to serve as base fertilizer. After application, the ecological balance of the soil is regulated by means of nutrient competition, metabolite regulation and control and the like, the fertility of the soil is enhanced, and the occurrence of soil-borne diseases is reduced. The microbial agent is simple in preparation method, readily available in raw materials, low in production cost, long in shelf life and obvious in disease prevention and yield increase effects.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a multifunctional Streptomyces freundii and its application as a solid bacterial agent, and further its application in preventing and treating soil-borne diseases of vegetables in protected areas and improving continuous cropping soil microecology. Background technique [0002] With the gradual expansion of protected cultivation areas, crop diseases are always an important factor affecting the production of protected areas. When serious, it will cause quality decline and yield reduction. Therefore, effective control of diseases is very important. Due to farmers' habit of planting the same crop, continuous cropping of a certain crop is caused. Excessive use of chemicals and fertilizers in special facilities destroys the ecological balance of the soil, resulting in serious soil-borne diseases and continuous cropping obstacles. In recent years, capsicum root rot has occurred seriously in pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20A01G13/00A01G7/06A01N63/28A01P3/00C12R1/54
CPCC12N1/20C05F17/20A01G13/00A01G7/06A01N63/28Y02W30/40Y02A40/10
Inventor 高晓梅池景良李杨敖静刘晓辉桓明辉孙玉禄郭玲玲
Owner 辽宁省微生物科学研究院
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