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Production method capable of increasing yield of neomycin

A production method and technology of neomycin, which are applied in the production field of increasing the output of neomycin, can solve the problems of production yield and production cycle limit, complex production process, long cycle and the like, and achieve small changes in production process and low production cost. , the effect of increasing production

Active Publication Date: 2014-04-23
福安药业集团烟台只楚药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, due to the complex production process, long cycle and low yield
The limitations of the development of the process itself, the production yield and production cycle seem to have reached the limit

Method used

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  • Production method capable of increasing yield of neomycin
  • Production method capable of increasing yield of neomycin
  • Production method capable of increasing yield of neomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Soybean meal powder 0.5, peanut meal powder 2.8, rice flour 5.0, yeast powder

[0044] 0.9, glucose 1.5, peptone 1.0, ammonium sulfate 0.5, sodium chloride 0.45, light calcium carbonate 0.45, KH 2 PO 4 0.06, α-amylase 0.02, soybean oil 0.12, and silicon oil 0.05 were added to the conventional fermentation medium of Streptomyces freundii, with lysine added at a concentration of 0.3 g / L, and inoculated with Streptomyces fradiae-H strain according to the conventional method ( SY060112), fermented for 130 hours at pH 7.0, culture temperature 35°C and volume 50 liters.

Embodiment approach

[0046] (1) Incline medium% (g / 100ml) formula: glucose 1.0, beef extract 0.2, agar 1.8~2.2, dipotassium hydrogen phosphate 0.05, DL-aspartin 0.05

[0047] (2) Seed bottle culture medium% (g / 100ml) formula: glucose 2.0, DL-asparagus 0.06, peptone 0.5, peanut meal powder 2.8, yeast powder 0.5, rice flour 8.0, corn steep liquor 0.25, amylase 0.0025, sulfuric acid Ammonium 0.6, light calcium carbonate 0.45

[0048] (3) Reproduction tank seed medium% (g / 100ml) formula: peanut meal 1.0, corn starch 1.0, yeast powder 2.25, glucose 2.5, peptone 0.5, ammonium sulfate 0.1, light calcium carbonate 1.4, Na 2 HPO 4 0.1, soybean oil 0.28

[0049] (4) Fermentation tank fermentation medium% (g / 100ml) formula: soybean meal 0.5, peanut meal 2.8, rice flour 5.0, yeast powder 0.9, glucose 1.5, peptone 1.0, ammonium sulfate 0.5, sodium chloride 0.45 light Calcium carbonate 0.45, KH 2 PO 4 0.06, α-amylase 0.02, soybean oil 0.12, silicone oil 0.05, lysine concentration 0.03

[0050] (5) Preparation of incli...

Embodiment 2

[0060] Example 2: The additive is ferrous lactate with a concentration of 0.6 g / L, instead of the lysine concentration of 0.3 g / L in Example 1. The medium composition and dosage, and fermentation conditions are the same as in Example 1.

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PUM

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Abstract

The invention relates to a production method capable of increasing yield of neomycin. According to the production method, one or several of additives which are lysine, ferrous lactate, tyrosine, methionine, hydrolysis casein, vitamin B12, fructose, glucosamine, folic acid, riboflavin and tryptophan are added to a conventional fermentation medium comprising peanut meal, corn starch, rice flour, yeast powder, glucose, peptone, ammonium sulfate, sodium chloride, light calcium carbonate and KH2PO4-containign streptomyces fradiae. The production method has the advantages that the yield of the neomycin is increased by above 5-20%, the cost is reduced, and the fermentation efficiency is high. The neomycin is used for treating human diseases, widely used in animal husbandries and used as antibiotics.

Description

Technical field [0001] The present invention provides a production method of neomycin, specifically, a production method for improving the production of neomycin. It is a preparation method that involves using microorganisms to ferment and increase the production of neomycin. Background technique [0002] Neomycin is an aminoglycoside antibiotic. Neomycin is the first 2-DOS (2-deoxystreptamine) aminoglycoside antibiotic to be discovered. It is an amino cyclic polysaccharide isolated from the metabolites of Streptomyces fradiae by Waksman et al. in 1949. The alcohol compound antibiotic mainly binds to the 30S subunit of the bacterial ribosome and inhibits the bacterial synthesis of protein. Streptomyces fradiae strains can be purchased commercially and are also strains used in the domestic neomycin microbial fermentation production process. Clinically commonly used neomycin sulfate (Neomycin Salfate), molecular formula: C23H46N6O13.3 (H2SO4). The product is white or slightly y...

Claims

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Application Information

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IPC IPC(8): C12P19/52C12R1/54
Inventor 尹国民齐红革郑朝霞刘洪海唐茂峰
Owner 福安药业集团烟台只楚药业有限公司
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