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Composite microbial agent used for soil amelioration and preparation method of composite microbial agent

A compound microbial agent and soil improvement technology, which is applied in the field of agricultural microorganisms, can solve the problems of unstable use effect and single performance of the bacterial agent, and achieve the effects of eliminating compaction, overcoming the obstacles of repeated cropping, and reducing the use of pesticides

Inactive Publication Date: 2018-08-14
SHANDONG CREATE YIFENG FERTILIZER GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the microbial agents used in agriculture are produced by the cultivation of a single strain. Because the performance of a single strain of the agent is single, the use effect is not very stable, so it is urgent to screen through the combination of strains and agents, cultivate and produce The effect is stable, which can not only increase crop production, reduce the use of chemical fertilizers, but also improve soil and crop quality and reduce pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A compound microbial agent for improving soil, including acidophilus Thiobacillus ferrooxidans 2.5×10 8 cfu / g, Pseudomonas fluorescens 5.0×10 8 cfu / g, Streptomyces freundii 4.0×10 8 cfu / g, jelly-like Bacillus 4.0×10 8 cfu / g, Paecilomyces lilacinus 4.5×10 8 cfu / g, paracoccus denitrification 3.5×10 8 cfu / g and Bacillus licheniformis 5.0×10 8 cfu / g.

[0033] Its preparation process is as follows:

[0034] (1) Inoculate each of the above bacterial strains into the activation medium at 30°C and 200r / min, and cultivate for 48 hours to obtain the seed liquid of each bacterial strain;

[0035] (2) Inoculate the seed liquids of the various strains into the fermentation medium respectively under the conditions of 30° C., 200 r / min, and pH=7.0, and cultivate and diffuse for 48 hours to obtain the fermentation liquid of each strain;

[0036] (3) After mixing the fermented liquids of the various strains, carry out constant temperature drying at 30°C;

[0037] (4) After dryin...

Embodiment 2

[0040] A compound microbial agent for improving soil, including acidophilus Thiobacillus ferrooxidans 3.0×10 8 cfu / g, Pseudomonas fluorescens 4.0×10 8 cfu / g, Streptomyces freundii 3.5×10 8 cfu / g, jelly-like Bacillus 3.5×10 8 cfu / g, Paecilomyces lilacinus 5.0×10 8 cfu / g, paracoccus denitrification 4.0×10 8 cfu / g and Bacillus licheniformis 4.5×10 8 cfu / g.

[0041] Its preparation process is as follows:

[0042] (1) Inoculate each of the above-mentioned strains into the activation medium at 33°C and 180 r / min, and cultivate for 48 hours to obtain the seed liquid of each strain;

[0043] (2) inoculate the seed liquids of the various strains into the fermentation medium respectively under the conditions of 33° C., 180 r / min, and pH=7.1, and cultivate and diffuse for 48 hours to obtain the fermentation liquids of the various strains;

[0044] (3) After mixing the fermentation broths of the various strains, carry out constant temperature drying at 33°C;

[0045](4) After dryi...

Embodiment 3

[0048] A compound microbial agent for improving soil, including acidophilus Thiobacillus ferrooxidans 4.0×10 8 cfu / g, Pseudomonas fluorescens 6.0×10 8 cfu / g, Streptomyces freundii 3.0×10 8 cfu / g, jelly-like Bacillus 3.0×10 8 cfu / g, Paecilomyces lilacinus 5.5×10 8 cfu / g, paracoccus denitrification 4.5×10 8 cfu / g and Bacillus licheniformis 4.0×10 8 cfu / g.

[0049] Its preparation process is as follows:

[0050] (1) Inoculate each of the above bacterial strains into the activation medium at 35°C and 150 r / min, and cultivate for 48 hours to obtain the seed liquid of each bacterial strain;

[0051] (2) Inoculate the seed liquids of the various strains into the fermentation medium respectively under the conditions of 35° C., 150 r / min, and pH=7.2, and cultivate and diffuse for 48 hours to obtain the fermentation liquid of each strain;

[0052] (3) After mixing the fermented liquids of the various strains, carry out constant temperature drying at 35°C;

[0053] (4) After dryi...

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PUM

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Abstract

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a composite microbial agent used for soil amelioration and a preparation method of the composite microbial agent. The composite microbial agent used for the soil amelioration includes acidithiobacillus ferrooxidans, pseudomonas fluorescens, streptomyces fradiae, bacillus mucilaginosus, paecilomyces lilacinus, paracoccus denitrificans and bacillus licheniformis. The composite microbial agent provided by the invention can quickly form a PGRR protective layer on the soil surface, reduce harmful microbial content, repair soil biological-ecological balance, enhance soil activity, stress resistance and soil fertility, decompose and activate large, medium and micro nutrient elements insolublein water in soil, improve an absorption and utilization rate of chemical fertilizers, greatly improve fertilizer efficiency, and reduce use of pesticides.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a composite microbial bacterial agent for soil improvement and a preparation method thereof. Background technique [0002] Soil is the material basis for the growth of crops. The amount of soil organic matter, the strength of soil fertility, the quality of soil aeration and the balance of soil solution play a decisive role in the growth of plants. Soil is mainly composed of minerals, organic matter and microorganisms. The activity of soil microorganisms is very important for the nutrition of plant roots, because the beneficial microorganisms in the soil are directly involved in the formation of soil fertility, including the transformation of substances and energy in the soil, humus The formation and decomposition of nutrients, the release of nutrients, the fixation of nitrogen, etc., but the number of beneficial microorganisms is not enough in a p...

Claims

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Application Information

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IPC IPC(8): C09K17/00C12N1/20C12N1/14C09K101/00C12R1/01C12R1/39C12R1/54C12R1/07C12R1/79C12R1/10
CPCC09K17/14C09K2101/00C12N1/14C12N1/20
Inventor 刘亚民
Owner SHANDONG CREATE YIFENG FERTILIZER GRP CO LTD
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