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Insecticidal biological composite inocula and preparation method thereof

A compound bacterial agent and compound bacteria technology, applied in the field of agricultural microorganisms, can solve the problems of low survival rate of effective bacteria, limited insecticidal range, short survival time, etc. rate effect

Inactive Publication Date: 2018-08-14
SHANDONG CREATE YIFENG FERTILIZER GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the biological composite bacterial agents used in agriculture mainly have the disadvantages of limited insecticidal range, low insecticidal rate, low survival rate of effective bacteria, and short survival time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of insecticide biological composite bacterial agent, comprises 0.3% of composite bacteria, 0.1% of strain protection agent and 99.6% of carrier by weight ratio, and composite bacteria is Bacillus thuringiensis, Paecilomyces lilacinus, Beauveria bassiana, Pseudomonas fluorescens, Streptomyces freundii, Bacillus jellylike, Bacillus licheniformis and Streptomyces hygroscopicus.

[0035] Among them, Bacillus thuringiensis: Paecilomyces lilacinus: Beauveria bassiana: Pseudomonas fluorescens: Streptomyces freundii: Bacillus jellylike: Bacillus licheniformis: Streptomyces hygroscopicus = 0.8: 1.2: 1.0 : 1.25: 0.8: 0.8: 1.2: 1.0; the strain protection agent is glucose, activated carbon and skimmed milk powder, and by weight, glucose: activated carbon: skimmed milk powder=2: 1: 5.

[0036] Its preparation process is as follows:

[0037] (1) Inoculate each of the above bacterial strains into the activation medium at 30°C and 200r / min, and cultivate for 48 hours to obtain ...

Embodiment 2

[0043] A kind of insecticide biological composite bacterial agent, comprises 0.4% of composite bacteria, 0.3% of strain protection agent and 99.3% of carrier by weight ratio, and composite bacteria is Bacillus thuringiensis, Paecilomyces lilacinus, Beauveria bassiana, Pseudomonas fluorescens, Streptomyces freundii, Bacillus jellylike, Bacillus licheniformis and Streptomyces hygroscopicus.

[0044] Among them, Bacillus thuringiensis: Paecilomyces lilacinus: Beauveria bassiana: Pseudomonas fluorescens: Streptomyces freundii: Bacillus jellylike: Bacillus licheniformis: Streptomyces hygroscopicus = 0.8: 1.2: 1.0 : 1.25: 0.8: 0.8: 1.2: 1.0; the strain protection agent is glucose, activated carbon and skimmed milk powder, by weight, sucrose: activated carbon: skimmed milk powder=2: 1: 5.

[0045] Its preparation process is as follows:

[0046] (1) Inoculate each of the above-mentioned strains into the activation medium at 33°C and 180 r / min, and cultivate for 48 hours to obtain the...

Embodiment 3

[0052] A biocomposite bacterial agent for insecticide, comprising 0.5% of composite bacteria, 0.5% of strain protectant and 99.0% of carrier by weight ratio, and the composite bacteria are Bacillus thuringiensis, Paecilomyces lilacinus, Beauveria bassiana, Pseudomonas fluorescens, Streptomyces freundii, Bacillus jellylike, Bacillus licheniformis and Streptomyces hygroscopicus.

[0053] Among them, Bacillus thuringiensis: Paecilomyces lilacinus: Beauveria bassiana: Pseudomonas fluorescens: Streptomyces freundii: Bacillus jellylike: Bacillus licheniformis: Streptomyces hygroscopicus = 0.8: 1.2: 1.0 : 1.25: 0.8: 0.8: 1.2: 1.0; the strain protection agent is glucose, activated carbon and skimmed milk powder, and by weight, lactose: activated carbon: skimmed milk powder=2: 1: 5.

[0054] Its preparation process is as follows:

[0055] (1) Inoculate each of the above bacterial strains into the activation medium at 35°C and 150 r / min, and cultivate for 48 hours to obtain the seed li...

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PUM

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Abstract

The invention belongs to the technical field of agriculture microbe, and especially relates to an insecticidal biological composite inocula and a preparation method thereof. The insecticidal biological composite inocula comprises composite bacteria, a bacterial strain protective agent, and a carrier; and the composite bacteria is bacillus thuringiensis, paecilomyces lilacinus, beauveria bassiana,pesudomonas fluorescence, streptomyces fradiae, bacillus mucilaginosus, bacillus licheniformis and hygrospinocus var.beigingenis. The insecticidal biological composite inocula has the advantages of wide insecticidal scope, less amount, and low cost, can effectively inhibit harmful flora and insect breeding, can increase the pest-resistant capability of plants, can enhance the soil activity, stressresistance and soil fertility, increases the fertilizer absorption utilization rate, keeps high insecticidal rate, can keeps high survival rate of effective bacterial strains, and prolongs the viability time of the effective bacterial strains.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a biocomposite bacterial agent for insecticide and a preparation method thereof. Background technique [0002] Soil is the material basis for the growth of crops. The amount of soil organic matter, the strength of soil fertility, the quality of soil aeration and the balance of soil solution play a decisive role in the growth of plants. Soil is mainly composed of minerals, organic matter and microorganisms. The activity of soil microorganisms is very important for the nutrition of plant roots, because the beneficial microorganisms in the soil are directly involved in the formation of soil fertility, including the transformation of substances and energy in the soil, humus The formation and decomposition of nutrients, the release of nutrients, the fixation of nitrogen, etc., but the number of beneficial microorganisms is not enough in a purely natura...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/04A01N63/00A01N25/22A01P7/04A01P5/00A01P7/02C12R1/07C12R1/79C12R1/645C12R1/39C12R1/54C12R1/10C12R1/55
CPCA01N25/22A01N63/00A01N63/30C12N1/14C12N1/20A01N2300/00
Inventor 刘亚民
Owner SHANDONG CREATE YIFENG FERTILIZER GRP CO LTD
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