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Culture medium for producing neomycin by utilizing fermentation of streptomyces fradiae

A technology of Streptomyces flexneri and fermentation medium, which is applied in the field of medium for producing neomycin, can solve the problems of low yield, limited resources, and high raw material prices, achieve the improvement of mycelial growth and the ability to produce hormones, and promote the development of mycelium. Growth and metabolism, the effect of improving the viability of strains

Pending Publication Date: 2016-11-09
NINGXIA TAIYICIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1 In the traditional fermentation medium, peanut cake powder is used as the late-acting nitrogen source. Due to the influence of the place of origin and processing technology, the quality of raw materials fluctuates greatly, which affects the level of fermentation production
[0006] 2 The cost of fermentation raw materials is high
In the traditional fermentation medium, can rice flour is used as the carbon source, and can rice flour is produced from southern early rice, with low yield and limited resources, resulting in high raw material prices and high production costs
[0007] 3 The fermentation cycle is long, generally around 240h, resulting in higher energy consumption and increased production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Seed culture: the effective volume of seed medium is 2m 3 .

[0031] Seed medium: soluble starch 50kg, soybean peptone 24kg, yellow rice flour 12kg, biological fish meal 6kg, corn steep liquor 6kg, yeast powder 12kg, corn flour 40kg, glucose 40kg, (NH 4 ) 2 SO 4 18kg, light calcium carbonate 12kg.

[0032] First, sterilize the seed medium at 121°C, keep the pressure for 30 minutes, cool to 25°C, and keep the pressure with sterile air. The quality requirements of the sterilized seed medium are: amino nitrogen 64mg / 100ml, total sugar 3.2g / 100ml, pH6.9. Then, under the protection of the flame, the cultured streptomycin mother bottle fermentation broth was inserted into the seed medium according to the inoculum size of 0.1L for seed culture. The seed culture conditions were: tank pressure 0.03-0.05MPa; tank temperature 35±0.5℃; air flow 180m 3 / h; stirring speed 160r / min. To the cell concentration of 25.2%, pH 6.6, culture time 36h to transplant.

[0033] Fermenta...

Embodiment 2

[0038] Seed culture: the effective volume of seed medium is 2m 3 .

[0039] Seed medium: soluble starch 54kg, soybean peptone 24kg, yellow rice flour 14kg, biological fish meal 8kg, corn steep liquor 6kg, yeast powder 12kg, corn flour 40kg, glucose 40kg, (NH 4 ) 2 SO 4 18kg, light calcium carbonate 12kg.

[0040] First, sterilize the seed medium at 121°C, keep the pressure for 30 minutes, cool to 25°C, and keep the pressure with sterile air. The quality requirements of the sterilized seed medium are: amino nitrogen 69mg / 100ml, total sugar 3.4g / 100ml, pH7.3. Then, under the protection of the flame, the cultured streptomycin mother bottle fermentation broth was inserted into the seed medium according to the inoculum size of 0.1L for seed culture. The seed culture conditions were: tank pressure 0.03-0.05MPa; tank temperature 35±0.5℃; air flow 180m 3 / h; stirring speed 160r / min. The bacteria concentration was 28.6%, the pH value was 6.9, and the cultivation time was 34 ho...

Embodiment 3

[0046] Seed culture: the effective volume of seed medium is 2m 3 .

[0047] Seed medium: soluble starch 58kg, soybean peptone 24kg, yellow rice flour 16kg, biological fish meal 10kg, corn steep liquor 6kg, yeast powder 12kg, corn flour 40kg, glucose 40kg, (NH 4 ) 2 SO 4 18kg, light calcium carbonate 12kg.

[0048] First, sterilize the seed medium at 121°C, keep the pressure for 30 minutes, cool to 25°C, and keep the pressure with sterile air. The quality requirements of the sterilized seed medium are: amino nitrogen 75mg / 100ml, total sugar 3.5g / 100ml, pH7.4. Then, under the protection of the flame, the cultured streptomycin mother bottle fermentation broth was inserted into the seed medium according to the inoculum size of 0.1L for seed culture. The seed culture conditions were: tank pressure 0.03-0.05MPa; tank temperature 35±0.5℃; air flow 180m 3 / h; stirring speed 160r / min. The bacteria concentration was 27.6%, the pH value was 6.9, and the culture time was 34 hours...

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Abstract

The invention relates to a culture medium for producing neomycin by utilizing fermentation of streptomyces fradiae. The culture medium comprises a seed culture medium and a fermentation culture medium, wherein soy peptone, biological fish meal, millet meal, zinc lactate, ammonium molybdate and other substances are added into the culture medium, so that metabolism of strains can be benefited, fermentation starting titer can be improved, the aging speed of hyphae can be delayed, the neomycin production capability can be improved, the fermentation cycle can be shortened, the fermentation cost can be reduced, and high-efficiency production of neomycin can be realized.

Description

technical field [0001] The invention belongs to the field of fermentation technology, in particular to a medium for producing neomycin by fermenting Streptomycesfradiae. Background technique [0002] Neomycin is the first discovered 2-DOS (2-deoxystreptomyces amine) aminoglycoside antibiotic, which is a mixture isolated from the metabolites of Streptomyces fradiae by Waksman et al in 1949 , Contains A, B, C three components, mainly B, C. Chemical name: 2-deoxy-4-O-(2,6-diamino-2,6-dideoxy-α-D-glucopyranosyl)-5-O-[3-O-(2,6- Diamino-2,6-dideoxy-β-L-idopyranosyl)-β-D-ribofuranosyl]-D-streptamethamine sulfate. [0003] Neomycin sulfate has a strong bactericidal effect on Gram-negative bacteria and some Gram-positive bacteria. It has inhibitory effect on actinomycetes, leptospira and amoeba. Strong antibacterial effect in alkaline solution. Clinically, it is mainly used for the treatment of intestinal infection, skin infection, ENT diseases and disinfection before abdominal ...

Claims

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Application Information

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IPC IPC(8): C12P19/52C12R1/54
CPCC12P19/52
Inventor 裴立忠郭佳张江飞尤永福
Owner NINGXIA TAIYICIN BIOTECH CO LTD
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