Streptomyces fradiae solid culture medium, culture method and preparation method of agricultural microbial agent

A technology of Streptomyces flexneri and solid culture medium, which is applied in the field of microbial fermentation, can solve the problems of difficulty in preparing agricultural solid microbial inoculants, long fermentation period, low spore production, etc. The effect of promoting sporulation

Active Publication Date: 2016-11-23
KINGENTA ECOLOGICAL ENG GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a Streptomyces flexneri solid culture medium and its preparation and fermentation method, as well as a method for preparing the agricultural Streptomyces flexneri inoculant using the medium, so

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  • Streptomyces fradiae solid culture medium, culture method and preparation method of agricultural microbial agent
  • Streptomyces fradiae solid culture medium, culture method and preparation method of agricultural microbial agent

Examples

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Embodiment 1

[0055] The preparation method of Streptomyces flexneri solid medium:

[0056] (A) By weight, weigh 10 parts of corn flour, 10 parts of soybean meal powder, 18 parts of straw powder, 30 parts of peat soil, 30 parts of phosphorus tailings and 2 parts of calcium carbonate, wherein the particle size of the straw powder is -40 mesh , The particle size of peat soil is -40 mesh. Mix all substances evenly;

[0057] (B) Add 45 parts of sucrose-ammonium sulfate nutrient solution, mix thoroughly with a stainless steel stirring system, and add 1.2kg / cm2 at 115°C 2 Pressure sterilize for 40 minutes to obtain a solid medium. The composition of the sucrose-ammonium sulfate nutrient solution includes in parts by weight: 1 part of sucrose, 1 part of ammonium sulfate, 0.6 part of potassium dihydrogen phosphate and 92 parts of water.

[0058] Fermentation method of Streptomyces flexneri spores:

[0059] 1) The spores on the slant of the strain were transferred to the slant of the eggplant bo...

Embodiment 2

[0065] The preparation method of Streptomyces flexneri solid medium:

[0066] (A) By weight, weigh 7 parts of corn flour, 15 parts of soybean meal powder, 20 parts of straw powder, 20 parts of peat soil, 35 parts of phosphorus tailings and 3 parts of calcium carbonate, wherein the particle size of peat soil is -40 mesh , The particle size of corn stalk powder is -30 mesh. Mix all substances evenly;

[0067] (B) Add 43 parts of sucrose-ammonium sulfate nutrient solution, mix thoroughly with a stainless steel stirring system, and add 1.5kg / cm2 at 122°C 2 Pressure was applied for 20 minutes to obtain a solid medium. The composition of the sucrose-ammonium sulfate nutrient solution includes in parts by weight: 5 parts of sucrose, 3 parts of ammonium sulfate, 1 part of potassium dihydrogen phosphate and 85 parts of water.

[0068] Fermentation method of Streptomyces flexneri spores:

[0069] Same as Example 1, the difference is that after the spore suspension is inoculated into...

Embodiment 3

[0073] The preparation method of Streptomyces flexneri solid medium:

[0074] (A) By weight, weigh 5 parts of corn flour, 8 parts of soybean meal powder, 25 parts of straw powder, 25 parts of peat soil, 40 parts of phosphorus tailings and 2 parts of calcium carbonate; wherein the particle size of peat soil is -40 mesh , The particle size of corn stalk powder is -50 mesh. Mix all substances evenly;

[0075] (B) Add 48 parts of sucrose-ammonium sulfate nutrient solution, mix thoroughly with a stainless steel stirring system, and add 1.3kg / cm2 at 120°C 2 Pressure for 30 minutes to obtain a solid medium. The composition of the sucrose-ammonium sulfate nutrient solution includes in parts by weight: 7 parts of sucrose, 2 parts of ammonium sulfate, 0.8 parts of potassium dihydrogen phosphate and 89 parts of water.

[0076] Fermentation method of Streptomyces flexneri spores:

[0077] Same as Example 1, the difference is that after the spore suspension is inoculated into the liqui...

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Abstract

The invention provides a streptomyces fradiae solid culture medium, a culture method and a preparation method of an agricultural microbial agent, belonging to the field of microbial fermentation. The streptomyces fradiae solid culture medium is prepared from the following components in parts by weight: 3-10 parts of corn flour, 3-18 parts of soybean meal, 18-45 parts of straw powder, 10-30 parts of turfy soil, 20-50 parts of phosphate tailing, 1-10 parts of calcium carbonate and 40-50 parts of nutrient solution. According to the invention, a solid fermentation culture medium is prepared through liquid-solid two-phase fermentation by adopting the solid culture medium and is used for producing an agricultural streptomyces fradiae agent. The fermentation culture medium for streptomyces fradiae spore production, provided by the invention, is suitable for the growth of streptomyces fradiae, and over 7.5*10<9> spores can be produced from each gram of fermentation raw material; and the germination rate of the prepared spore powder can reach 95%, the prepared agricultural microbial agent conforms to the national standard of agricultural microbial agent, and an obvious promotion effect is realized on the crop growth.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a Streptomyces flexneri solid culture medium, a preparation and fermentation method thereof, and a method for preparing an agricultural Streptomyces flexneri bacterial agent using the culture medium. Background technique [0002] Streptomycetaceae (Streptomycetaceae) is the main producer of antibiotics. Among the more than 20,000 active substances produced by microorganisms, Streptomycetaceae accounts for more than 60%. Application prospects. As a species of Streptomyces, Streptomyces freundii can produce effective active ingredients such as tylosin and neomycin, which have obvious growth-promoting and disease-resistant effects on crops, and are increasingly used in Various crops such as fruit trees and vegetables are being cultivated. [0003] However, the amount of Streptomyces flexneri growing naturally in the soil is too small to perform the above-mentioned effects. I...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G1/00C12R1/54
CPCC05B17/00C12N1/20C05F11/00C05F11/02
Inventor 胡兆平李新柱邢芳芳高明夫禚优优
Owner KINGENTA ECOLOGICAL ENG GRP
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