Culture medium for producing tylosin by fermenting streptomyces fradiae, and fermenting method

The technology of Streptomyces flexneri and fermentation medium is applied in the culture medium and fermentation field of Streptomyces flexneri to produce tylosin by fermentation, which can solve the problem of increasing the work intensity of bacteria researchers, high production cost of tylosin, The problems of long fermentation cycle of tylosin can shorten the production cycle, reduce the types and dosage, and reduce the adverse factors.

Active Publication Date: 2014-01-01
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1 There are 4 types of organic nitrogen sources in tylosin seeds and fermentation medium, which increases the purchase cost of enterprises
[0005] 2 The first-level seed inoculation amount is more
The inoculum volume of conventional primary seed culture is 5-10L/m 3 , increasing the work intensity of st

Method used

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  • Culture medium for producing tylosin by fermenting streptomyces fradiae, and fermenting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Prepare 1L of shake flask seed medium, including 3ml of soybean oil, 5g of brewer's yeast residue, 4g of earthworm powder, 2g of dry corn steep liquor powder, 2g of magnesium sulfate, 1g of iron sulfate, 2g of light calcium carbonate, and 2g of ammonium sulfate.

[0074] First, pour earthworm powder and beer yeast dry residue into a stainless steel pot, add drinking water with an actual volume of 400ml, boil for 15 minutes and cool to ambient temperature, then add magnesium sulfate, iron sulfate and ammonium sulfate, and finally pour corn steep liquor dry powder into a beaker, After adding drinking water to dissolve, pour it into the above-mentioned stainless steel pot, and make up to 1000ml with drinking water. The pH was adjusted to 7.1 with 20% NaOH. Add light calcium carbonate to the above culture medium and stir for 3 minutes, measure 250 ml of the culture medium with a graduated cylinder and distribute it in a 750 ml Erlenmeyer flask with 3 ml of soybean oil added...

Embodiment 2

[0079] Prepare 1L of shake flask seed medium, including 4ml of corn oil, 6g of brewer's yeast residue, 5g of earthworm powder, 3g of corn steep liquor powder, 2.2g of magnesium sulfate, 1.3g of iron sulfate, 2.3g of light calcium carbonate, and 2.2g of ammonium sulfate.

[0080] First, pour earthworm powder and brewer’s yeast dry residue into a stainless steel pot, add drinking water with an actual volume of 400ml, boil for 16 minutes and cool to ambient temperature, then add magnesium sulfate, iron sulfate and ammonium sulfate, and finally pour corn steep liquor dry powder into a beaker, After adding drinking water to dissolve, pour it into the above-mentioned stainless steel pot, and make up to 1000ml with drinking water. The pH was adjusted to 7.3 with 20% NaOH. Add light calcium carbonate to the above culture medium and stir for 4 minutes, measure 250 ml of the culture medium with a graduated cylinder and distribute it in a 750 ml Erlenmeyer flask with 4 ml of soybean oil ...

Embodiment 3

[0085] Prepare 1L of shake flask seed medium, including 4.5ml of soybean oil, 7g of brewer's yeast residue, 6g of earthworm powder, 3.5g of corn steep liquor powder, 2.5g of magnesium sulfate, 2g of iron sulfate, 3g of light calcium carbonate, and 3g of ammonium sulfate. First, pour earthworm powder and beer yeast dry residue into a stainless steel pot, add drinking water with an actual volume of 400ml, boil for 17 minutes and cool to ambient temperature, then add magnesium sulfate, iron sulfate and ammonium sulfate, and finally pour corn steep liquor dry powder into a beaker, After adding drinking water to dissolve, pour it into the above-mentioned stainless steel pot, and make up to 1000ml with drinking water. The pH was adjusted to 7.5 with 20% NaOH. Add light calcium carbonate to the above culture medium and stir for 4 minutes, measure 250 ml of the culture medium with a graduated cylinder and distribute it in a 750 ml Erlenmeyer flask with 4.5 ml of soybean oil added in a...

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Abstract

The invention relates to a culture medium for producing tylosin by fermenting streptomyces fradiae, and a fermenting method. A shaking bottle seed culture medium, a first-level seed culture medium, a second-level seed culture medium and a fermentation medium all comprise beer yeast dry residue and earthworm powder. According to the invention, the formula of the culture medium is optimized, so that the problem about the costs of raw materials and auxiliary materials is solved, the influence on the sources of the raw materials and auxiliary materials is reduced to the maximum degree, sufficient supply of the raw materials and auxiliary materials is guaranteed, and stable and high-efficiency production of the tylosin is realized. By adopting the culture medium the fermentation unit can be increased, the fermentation period can be shortened, the inoculation amount of primary seeds is reduced, and the quantity of the component A of the tylosin is increased.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and a fermentation method for fermenting and producing tylosin by Streptomyces flexneri. Background technique [0002] Tylosin is a macrolide antibiotic obtained by fermentation and extraction of Actinomycetes Streptomyces freundii. It is mainly used as an animal-specific antibiotic. [0003] At present, the method of domestic production of tylosin is mainly a three-stage fermentation mode, using Streptomyces freundii as the strain, and there are mainly two modes of nitrogen sources in the traditional medium: one is based on fish meal, corn steep liquor, peanut cake powder An animal nitrogen source composed of yeast powder, and a vegetable nitrogen source composed of peanut cake powder, cottonseed cake powder, corn steep liquor and yeast powder. The main problems of the above culture medium are: [0004] 1 There are 4 types of organic nitrogen ...

Claims

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Application Information

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IPC IPC(8): C12P19/62C12R1/54
Inventor 任勇王义奇乃董媛李小萍
Owner 宁夏泰瑞制药股份有限公司
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