76results about How to "Small amount of inoculum" patented technology

The invention relates to a culture medium for fermentation production of spectinomycin through streptomyces spectabilis and a fermentation method. A primary seed culture medium, a secondary seed culture medium and a fermentation culture medium all contain corn oil, maltose, beer yeast dry residues and earthworm meal. According to the invention, maltose replaces glucose, beer yeast dry residues and earthworm meal replace fish meal, peptone, soybean cake meal and corn steep liquor, and the culture medium formula is optimized, so that the problem of high costs of raw materials is solved, the environmental effect on the source of the raw materials is reduced to the utmost extent, sufficient supply of the raw materials is guaranteed, and stable and efficient production of spectinomycin is realized. Meanwhile, the culture medium can improve the fermentation unit and shorten the fermentation period.

The invention provides a method for producing the liquid strain of cordyceps militaris. The method comprises the following steps: (1) inoculating the mother culture of cordyceps militaris to a dedicated strain culture medium to carry out the shaking culture; (2) moving the strain solution cultured in step (1) into a dedicated strain culture medium to carry out the amplification culture, wherein the inoculation amount accounts for 2% to 20% of the volume of the culture medium; (3) filtering the strain solution cultured in step (2) by using a sieve with the bore diameter thereof being 50 to 500 meshes to obtain the filtrate containing more than 107 conidia per ml; and (4) diluting the filtrate obtained in step (3) by using sterile water until the density thereof is 105 to 107 conidia per ml to obtain the liquid strain of cordyceps militaris. The method of the invention is applicable to inoculating and cultivating the fruit body of cordyceps militaris, wherein the dedicated strain culture medium contains carbon source, yeast nitrogen base, magnesium sulfate, monopotassium phosphate, dipotassium phosphate and agar.

The invention belongs to the technical field of wine brewing, and particularly relates to an amplification culture method of composite caproic acid bacteria liquid. In allusion to the problems that during existing caproic acid bacteria culture, the culture cycle is long, the bacterial colony structure is single, the caproic acid yield is low, the large-scale degree and the mechanization degree arelow, miscellaneous bacteria are easily caused, the quality is unstable and the like, the invention provides the amplification culture method of the composite caproic acid bacteria liquid. The amplification culture method comprises the following steps: a, activating a caproic acid bacteria, and performing proliferation culture; b, performing amplification culture on caproic acid bacteria; c, performing amplification culture on kiln mud enrichment liquid; d, performing mixed fermentation culture; e, performing mixed amplification culture. Through adoption of mixed culture of the pure caproic acid bacteria and the high-quality kiln mud enrichment liquid, the composite caproic acid bacteria liquid with rich bacterial colonies is obtained; the composite caproic acid bacteria liquid is stable in quality, uses the caproic acid bacteria as dominant bacteria, and has a very strong caproic acid producing capability; in addition, the culture amount is increased, the culture steps are reduced, the operating process is simplified, and the culture cycle is shortened, so that the production efficiency is improved and the production cost is saved.

The invention discloses a method for removing heavy metal Cu in sludge of an urban sewage treatment plant in a bioleaching mode, by means of the method, the problem that sludge of the urban sewage treatment plant contains much heavy metal Cu is solved, and the treated sludge can serve as fertilizer for agricultural utilization. The method includes the following specific steps that original thiobacillus in the sludge is enriched and cultured, acclimated sludge is obtained, the acclimated sludge serves as the inoculum and is added to raw sludge, Na2S2O3 and FeSO4 substrates are fed, the feeding amount of Na2S2O3 is controlled to be 3-5 g/L, the feeding amount of FeSO4 is controlled to be 4-6 g/L, air is blown into the sludge at the speed of 1.0-1.5 L/(min-L), stirring is conducted at a certain rotation speed, the temperature is controlled to be 20-30 DEG C, and the sludge is subjected to bioleaching; after bioleaching is conducted for 3-4 days, the removal rate of the heavy metal Cu reaches 90% or above. The method is reasonable in design and high in practicability.

The invention relates to a direct vat set clostridium butyricum starter and a preparation method thereof. The method comprises the following steps of: performing clostridium butyricum seed culture; performing high-density scale-up culture; preparing thallus starter by using a freeze-drying method; and finally obtaining the direct vat set clostridium butyricum starter, wherein one liter of culturemedium for clostridium butyricum seed culture and high-density scale-up culture is prepared from 1.75-2g of peptone, 0.5g of glucose, 0.02g of MgSO4, 0.5g of K2HPO4 and 0.02g of MnSO4; and concentration conditions for preparing the thallus starter by using the freeze-drying method are that: the centrifugal rotation speed is 5,000r/min, centrifugation time is 10 minutes, centrifugation temperatureis 4 DEG C, and a freeze-dry protecting agent comprises 10 percent dry skim milk and 10 percent malt extract. The survival rate of the direct vat set clostridium butyricum starter produced by the method is 94.6 percent, the viable count is 3.92*10<10>cfu/g, and an ideal liquid-state and solid-state fermentation effect can be achieved according to inoculum size of 0.1 percent. A novel preparation method for the high-activity direct vat set clostridium butyricum starter is developed on the basis of the strain to be applied to fermentative culture of clostridium butyricum.

Potato yoghourt and a making method thereof are disclosed. 40-60% of potato juice is creatively added into yoghourt, and lactobacillus bulgaricus and streptococcus thermophilus are used in cooperation for fermentation. On the basis of controlling the ratio of the materials and the making method, the potato yoghourt with rich nutrients, moderate sweetness and palatable sour taste is prepared.

The invention relates to a novel biological floc. The biological floc includes microalgae aggregates and commensalism heterotrophic bacteria, and the commensalism heterotrophic bacteria are Pesudomonas sp. and Bacillus sp. The biological floc significantly improves the water quality of the nursing pond of the litopenaeus vannamei, improves the survival rate, growth index and immunity of the litopenaeus vannamei, reduces the application amount of compound feed and external carbon sources, and reduces the aeration intensity and quantity of exchanged water.

The invention relates to an oligotrophic low-temperature denitrifying bacterium and its application so as to solve the problem that an existing denitrifying bacterium is not adapted to oligotrophic low-temperature water. The oligotrophic low-temperature denitrifying bacterium refers to janthinobacterium ((Janthinobacterium sp.) M-11, and is preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3 institution, No.1 Beichen west road, Chaoyang district, Beijing city, the preservation data is in July 3, 2017, and the preservation number is CGMCC No.14380. The application refers to the application of the denitrifying bacterium in denitrifying oligotrophic low-temperature water. Compared with other strains, the inoculation quantity of the oligotrophic low-temperature denitrifying bacterium is smaller, and the oligotrophic low-temperature denitrifying bacterium is more suitable for the treatment of oligotrophic water. The oligotrophic low-temperature denitrifying bacterium is applied to the technical field of environmental microorganisms.

The invention provides Bacillus sp. NB-1 and a culture method and application thereof. The Bacillus sp. NB-1 is Bacillus sp. NB-1, is preserved in the China Center for Type Culture Collection on July13 , 2020, the address is Wuhan University, Wuhan Province, China, and the preservation number is CCTCC NO: M2020304; the Bacillus sp. NB-1 is cultured for 24h at 30 DEG C by using an LB liquid culture medium; and the Bacillus sp. NB-1 is used for degrading the concentration of ammonia nitrogen and nitrite in aquaculture water and aquaculture tail water. The Bacillus sp. NB-1 can obviously reduce the concentration of ammonia nitrogen and nitrite in the aquaculture water and the aquaculture tail water, is harmless to people and cultured animals, does not generate secondary pollution, and can be applied to improvement of aquaculture environment and aquaculture tail water emission reduction in large-scale production.

The invention relates to a method for preparing a directed vat set bacillus coagulans starter. The method comprises the following steps of: concentrating fermentation liquor of bacillus coagulans, wherein the concentration conditions of the fermentation liquor are that: the centrifugal rotating speed is 6,000r/min, the centrifugal time is 20min, and the centrifugal temperature is 4 DEG C; adding a protective agent, wherein the adopted protective agent is an aqueous solution which contains 5 percent m/m sucrose and 10 percent m/m maltodextrin; adding bacterial sludge into the aqueous solution of the protective agent; and drying by a vacuum drying method at the drying temperature of 60 DEG C under the drying pressure of 12,000Pa. The viable count of the obtained starter is 3.24*10<10>cfu/g; the sporation rate reaches 90.3 percent; and ideal liquid and solid fermentation effects can be achieved according to one thousandth of inoculation amount.

The invention relates to the technical field of microbial engineering, and particularly relates to acetobacter aceti and application thereof. Wherein the bacillus aceticus is lactobacillus aceticus LHZ-ST001, wherein a preservation unit is China General Microbiological Culture Collection Center (CGMCC for short), a preservation address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, a preservation date is September 10th, 2018, and a preservation number is CGMCC No.16448; the acetobacter aceti has high viable count in a fermentation culture medium, has strong acid and ethanol dehydrogenase producing capacity, has the capacity of being compounded with other lactic acid bacteria or saccharomycetes, has good tolerance to acid, and has the characteristic of high acid resistance.

The invention discloses a method for rapidly enriching a functional flora without production of foreign odor substances in pit mud and an application of the functional flora, belonging to the technical field of brewing microorganisms. According to the method, tested and screened aged pit mud with high quality is used as seed mud, and selected high-quality pit mud is subjected to step-by-step amplification culture by adopting a specific culture medium. The obtained flora is a caproic acid-producing flora taking a clostridium rumen flora as a main body, and the flora does not generate a mud odorsubstance, namely 4-methylphenol. The mixed flora obtained by using the method disclosed by the invention can be used for a solid fermented grain fermentation process of a pit-mud-free system; the content of beneficial flavor substances of fermented grains can be remarkably increased; the generation of bad flavor substances in the pit mud is avoided; thus, the quality of raw wine is improved. Themixed flora can also be used in a culture process of artificial pit mud and a daily pit mud maintenance process, so the caproic acid-producing main flora in a pit can be increased; pit mud aging is promoted; pit mud aging and hardening are prevented; meanwhile, generation of the mud odor substance, namely the 4-methylphenol is effectively reduced.

The invention relates to a method for rapidly and selectively breeding terramycin strains. The method comprises the following steps: a, test tube slant culture; b, preparation of spore suspension; c, isolated culture; d, fermentation culture; e, strain re-testing. Single colonies obtained through isolated culture are directly inoculated into a fermentation medium for fermentation culture, so that the titer of the terramycin obtained through screening is high, the titer validation time of the single colonies is shortened to 48-50h, and the working efficiency is greatly improved.

The invention discloses bacillus cereus and application. The bacillus cereus HC7 is preserved in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms and has a preservation number of CGMCC No. 21660. The bacillus cereus HC7 can be used for effectively repairing water mass and can be used for repairing black and smelly water mass, lowering concentrations of pollutants such as COD and ammonia nitrogen in water, improving the DO, ORP and transparency of the water mass and obviously improving the water quality of the water mass; and the strain is wide in temperature adaptation range, relatively high in environmental adaptation, good in environmental stability, low in water mass repairing inoculum size and low in application cost, can withstand low-temperature deamination and has a huge popularization value.

The invention discloses a method for producing biogas through anaerobic fermentation of a degradation straw raw material. The method concretely comprises the following steps: chopping the straw raw material to form 0.3-0.8 cm fragments; adding the chopped fragments into an anaerobic reactor as a fermentation material; adding a fermentation buffer solution to the fermentation material in the anaerobic reactor according to a weight/volume ratio of the fermentation material to the fermentation buffer solution of 1:(45-90); inoculating the anaerobic reactor with an inoculation material according to a weight/volume ratio of the fermentation material to the inoculation material of 1:(5-10) to make the final concentration of the fermentation material be 1-2%; and adding ampicillin into the anaerobic reactor to make the final concentration of the ampicillin be 1800-2200 U/ml, and carrying out fermentation at a controlled reaction temperature of 38-40 DEG C for 48-72 h to obtain the biogas. Compared with the prior art, the method disclosed in the invention has the advantages of effective increase of the yield of the biogas produced from the straw raw material by 55-60%, increase of the proportion of the biogas in cumulative gas production, and increase the biogas yield per unit straw raw material.