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Bacillus cereus and application

A technology of Bacillus cereus and inoculum, which is applied in the field of microorganisms, can solve the problems of uneven degradation effect, limited number of bacterial species, and undeveloped process technology, and achieves reduced application cost, less inoculum, and good environmental stability. Effect

Active Publication Date: 2021-08-06
SICHUAN JUNHE ENVIRONMENTAL PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the number of special microbial strains in black and odorous water bodies is limited, the degradation effect is uneven, and the related technology needs to be developed

Method used

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  • Bacillus cereus and application
  • Bacillus cereus and application
  • Bacillus cereus and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Screening of strains

[0047] 1. The enrichment of indigenous strains in black and odorous water bodies.

[0048] The water body and sediment in the black and odorous river of Fenghuang Ergou, Chengdu were sampled, and the samples were placed in a low-temperature storage box for transshipment.

[0049] Enrichment of indigenous bacterial strains in water: shake and mix well, take 20mL of water and add it to 150mL enrichment medium, culture in a constant temperature shaking box at 30°C, 140r / min for 2 days, take 20mL of culture solution and add 150mL of enrichment medium for cultivation, repeat three times That is, the enriched culture solution of the native bacterial strains in the water body is obtained.

[0050] Enrichment of indigenous bacterial strains in bottom mud: Take 5g of bottom mud into a conical flask filled with 150mL sterilized saline, add a few glass beads and stir magnetically for 30 minutes. Take 20mL of the stirred liquid and add it to 150mL enrich...

Embodiment 2

[0081] Effects of different physical and chemical factors on the effect of bacterial strains in remediating black and odorous water bodies

[0082] When exploring the influence of physical and chemical factors, the HC7 seed liquid in Example 1 was inoculated in the artificially simulated black and odorous water body. The initial values ​​of the artificially simulated black and odorous water body were COD 78 mg / L, ammonia nitrogen 16 mg / L, DO 0 mg / L, redox The potential is ORP-217mV, and the transparency is 13cm. According to the black and odorous water body discrimination standard in the "Black and Odorous Water Treatment Work Guidelines", it belongs to severe black and odorous water. After 8 days, samples were taken to measure the chemical oxygen demand (COD), ammonia nitrogen, dissolved oxygen (DO), oxidation-reduction potential (ORP), and transparency of the artificially simulated black and odorous water body, with three parallels for each group. Ammonia nitrogen was determ...

Embodiment 3

[0105] 1, the preparation of bacterium liquid: the HC7 seed liquid among the embodiment 1 is transferred in the LB culture medium with 10% (v / v) inoculum amount, 30 ℃ of constant temperature shaking boxes, 140r / min cultivates 10h, promptly obtains bacterium liquid .

[0106] 2. Method: Take the black and odorous water body. When the water temperature is 20-25°C, spray the bacteria liquid into the black and odorous water body by spraying. The inoculum amount of the bacteria liquid is 1% of the volume of the black and odorous water body before the restoration. 24h, then stop; 3 days later, spray the bacteria solution again, the inoculation amount of the bacteria solution is 1% of the volume of the black and odorous water body before repairing, and the water body is aerated for 24 hours on the day of spraying, and then stop; spray the bacteria solution 5 times in total (the inoculum amount of each bacteria solution Both are 1% of the volume of the black and odorous water body bef...

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Abstract

The invention discloses bacillus cereus and application. The bacillus cereus HC7 is preserved in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms and has a preservation number of CGMCC No. 21660. The bacillus cereus HC7 can be used for effectively repairing water mass and can be used for repairing black and smelly water mass, lowering concentrations of pollutants such as COD and ammonia nitrogen in water, improving the DO, ORP and transparency of the water mass and obviously improving the water quality of the water mass; and the strain is wide in temperature adaptation range, relatively high in environmental adaptation, good in environmental stability, low in water mass repairing inoculum size and low in application cost, can withstand low-temperature deamination and has a huge popularization value.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus cereus and its application. Background technique [0002] With the acceleration of urbanization and rapid economic development, more and more production wastewater and domestic sewage are discharged into rivers, resulting in varying degrees of pollution to rivers, among which the more serious is the phenomenon of black and odorous rivers. In my country, black and odorous water has become a common problem in recent decades. There are reports of black and odorous water in Nanfei River in Anhui, Wenruitang River in Zhejiang, Taihu Lake in Jiangsu and Chaohu Lake in Anhui. There are many hazards in black and odorous water bodies, which not only affect the image of the city, but also lead to the deterioration of the water ecosystem and affect the health of surrounding residents. Therefore, the research on the restoration technology of black and odorous water bodies i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/085
CPCC12N1/20C02F3/34Y02W10/10
Inventor 罗启贵邓俊张丹谢冀飞
Owner SICHUAN JUNHE ENVIRONMENTAL PROTECTION
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