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Oligotrophic low-temperature denitrifying bacterium and its application

A nutrient-poor, denitrifying bacteria technology, applied in bacteria, biological water/sewage treatment, microorganisms, etc., can solve the problem that denitrifying bacteria are not suitable for nutrient-poor and low-temperature water bodies

Active Publication Date: 2017-09-22
HARBIN INST OF TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that existing denitrification bacteria are not suitable for oligotrophic low-temperature water bodies, and provide an oligotrophic low-temperature denitrification bacterium and its application

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  • Oligotrophic low-temperature denitrifying bacterium and its application
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  • Oligotrophic low-temperature denitrifying bacterium and its application

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specific Embodiment approach 1

[0015] Embodiment 1: An oligotrophic low-temperature denitrification bacterium in this embodiment is Janthinobacterium sp. M-11, which is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation address is Beichen, Chaoyang District, Beijing No. 3, No. 1 Yard, West Road, the preservation date is July 3, 2017, and the preservation number is CGMCC No.14380.

[0016] In this embodiment, Janthinobacterium sp.M-11, a high-efficiency low-temperature resistant denitrification bacterium screened from the filler in the Chinese-style reactor for Songhua River water treatment, has been identified as a Gram-negative bacterium, a facultative anaerobic bacterium, and its growth temperature is 2-35°C, the optimum temperature is 25-30°C, the length of the bacteria is 1-1.5μm, the width is 0.5-1μm, short rod-shaped, without spores. The colonies are white translucent regular circles with a radius of 1-2 mm, moist and shi...

specific Embodiment approach 2

[0019] Embodiment 2: The application of an oligotrophic low-temperature denitrification bacterium in this embodiment refers to the application in denitrification of oligotrophic low-temperature water bodies.

[0020] In this embodiment, the oligotrophic low-temperature denitrification bacterium Janthinobacterium sp.M-11 can grow at 2°C, and the removal rate of ammonia nitrogen can reach more than 90% in 48 hours, and there is no accumulation of nitrite nitrogen and nitrate nitrogen; at 2°C The removal rate of nitrite and nitrate is more than 80%, and the accumulation of nitrite has no inhibitory effect on the bacteria, indicating that the inoculation amount of Janthinobacterium sp.M-11 is higher than that of other strains. Small, more suitable for the treatment of oligotrophic low temperature water.

specific Embodiment approach 3

[0021] Specific embodiment three: the difference between this embodiment and specific embodiment two is: the dosage of low-temperature denitrification bacteria in nutrient-poor low-temperature water body is 4×10 6 cfu / mL. Others are the same as in the second embodiment.

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Abstract

The invention relates to an oligotrophic low-temperature denitrifying bacterium and its application so as to solve the problem that an existing denitrifying bacterium is not adapted to oligotrophic low-temperature water. The oligotrophic low-temperature denitrifying bacterium refers to janthinobacterium ((Janthinobacterium sp.) M-11, and is preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3 institution, No.1 Beichen west road, Chaoyang district, Beijing city, the preservation data is in July 3, 2017, and the preservation number is CGMCC No.14380. The application refers to the application of the denitrifying bacterium in denitrifying oligotrophic low-temperature water. Compared with other strains, the inoculation quantity of the oligotrophic low-temperature denitrifying bacterium is smaller, and the oligotrophic low-temperature denitrifying bacterium is more suitable for the treatment of oligotrophic water. The oligotrophic low-temperature denitrifying bacterium is applied to the technical field of environmental microorganisms.

Description

technical field [0001] The invention relates to an oligotrophic low-temperature denitrification bacterium and its application. Background technique [0002] Nitrogen is a common pollutant in natural water bodies. Excessive accumulation will cause serious consequences such as eutrophication of water bodies and ecological system imbalance. Traditional denitrification methods mainly include physical, chemical and biological methods. Compared with physical and chemical methods, biological denitrification has the advantages of low cost, less secondary pollution, and good impact resistance. [0003] In northern China, especially in Northeast China, due to the low temperature of surface water in winter, it is not conducive to the growth of traditional nitrogen-removing bacteria, and because of the lack of nutrients such as C and N in surface water, it has brought great harm to traditional biological methods. Earth difficulty. Therefore, to screen out highly efficient bacteria th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F101/16C12R1/01
CPCC02F3/34C02F2101/163C02F2101/166C02F2101/16C02F2103/007C12R2001/01C12N1/205
Inventor 马军杨墨秦必达刘慧玲
Owner HARBIN INST OF TECH
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