Amplification culture method of composite caproic acid bacteria liquid

A technology for expanding the culture of caproic acid bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low scale and mechanization, long culture period of caproic acid bacteria, single bacterial group structure, etc. , to achieve the effects of reducing labor costs, shortening the training period, and simplifying the operation process

Inactive Publication Date: 2018-06-01
LUZHOU PINCHUANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problems to be solved by the present invention are: the existing culture of caproic acid bacteria has problems such as long culture period, single bacterial group structure, low caproic acid yield, low scale and mechanization degree, easy contamination of miscellaneous bacteria, unstable quality, etc.

Method used

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  • Amplification culture method of composite caproic acid bacteria liquid

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Embodiment 1

[0045] Embodiment 1 uses the inventive method to compound hexanoic acid bacteria liquid expansion culture

[0046] The caproic acid bacteria that have been activated, multiplied and expanded are fermented with 2% of the inoculum and 2% of the pit mud enrichment liquid that has been expanded and cultivated in the seed tank to obtain a compound caproic acid bacterial liquid. Clostridium proliferation medium was used for the activation and proliferation of caproic acid bacteria, ethanol sodium acetate medium was used for seed tank cultivation of caproic acid bacteria and pit mud functional bacteria, and compound organic medium was used for mixed fermentation.

[0047] The caproic acid strain activation method is as follows: take 5 test tubes with a size of 15 mm × 150 mm, use the Clostridium proliferation medium to make a test tube slope, and pick the preserved test tube strains with an inoculation loop under aseptic conditions, "Z" After inoculation with font streaks, place them...

Embodiment 2

[0054] Embodiment 2 expands the cultivation of compound caproic acid bacteria liquid with the inventive method

[0055] The caproic acid bacteria that have been activated, proliferated and expanded are fermented with 2% of the inoculum and 2% of the pit mud enrichment liquid that has been expanded and cultivated in the seed tank. After fermentation, the fermentation broth is inoculated into the compound Continuous culture in organic medium. Clostridium proliferation medium is used for the activation and proliferation of caproic acid bacteria, ethanol sodium acetate medium is used for seed tank cultivation of caproic acid bacteria and pit mud functional bacteria, and compound organic medium is used for mixed fermentation and continuous culture.

[0056] In this embodiment, the method for activating caproic acid strains is as follows: take 5 test tubes with a specification of 15 mm × 150 mm, use the Clostridium proliferation medium to make a test tube slope, and pick and store t...

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Abstract

The invention belongs to the technical field of wine brewing, and particularly relates to an amplification culture method of composite caproic acid bacteria liquid. In allusion to the problems that during existing caproic acid bacteria culture, the culture cycle is long, the bacterial colony structure is single, the caproic acid yield is low, the large-scale degree and the mechanization degree arelow, miscellaneous bacteria are easily caused, the quality is unstable and the like, the invention provides the amplification culture method of the composite caproic acid bacteria liquid. The amplification culture method comprises the following steps: a, activating a caproic acid bacteria, and performing proliferation culture; b, performing amplification culture on caproic acid bacteria; c, performing amplification culture on kiln mud enrichment liquid; d, performing mixed fermentation culture; e, performing mixed amplification culture. Through adoption of mixed culture of the pure caproic acid bacteria and the high-quality kiln mud enrichment liquid, the composite caproic acid bacteria liquid with rich bacterial colonies is obtained; the composite caproic acid bacteria liquid is stable in quality, uses the caproic acid bacteria as dominant bacteria, and has a very strong caproic acid producing capability; in addition, the culture amount is increased, the culture steps are reduced, the operating process is simplified, and the culture cycle is shortened, so that the production efficiency is improved and the production cost is saved.

Description

technical field [0001] The invention belongs to the technical field of brewing, and in particular relates to a method for expanding the culture of compound caproic acid bacteria liquid. Background technique [0002] Caproic acid bacteria is a kind of important pit mud functional bacteria. The caproic acid produced by its metabolism and the ethanol produced by yeast undergo esterification reaction, and the ethyl caproate produced is the key aroma substance that determines the quality of Luzhou-flavor liquor. Acid bacteria liquid is widely used in pit maintenance, esterification liquid production and artificial pit mud cultivation. [0003] The traditional methods of producing caproic acid bacteria liquid include pure caproic acid bacteria liquid culture and pit mud enrichment culture. The liquid culture of pure caproic acid bacteria has the advantages of small inoculum size, fast growth and reproduction of bacteria, and high caproic acid production. The cultivation cycle is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 刘淼林锋张宿义秦辉杨艳邱川峰李德林罗杰康承霞徐琼王加彬
Owner LUZHOU PINCHUANG TECH CO LTD
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