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Method for producing liquid strain of cordyceps militaris

A technology of Cordyceps militaris and liquid strains, applied in the fields of botany equipment and methods, horticulture, applications, etc., can solve problems such as the correlation of no conidia cultivation effects, etc., and achieve the effect of easy strain quality

Inactive Publication Date: 2010-10-20
和润通(重庆)供应链管理有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Literature research shows that there is no report on the use of conidia of Cordyceps militaris as a cultured fruiting body, and there is no study on the correlation between conidia and cultivation effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation method of Cordyceps militaris liquid strain, its specific steps are:

[0020] (1) Take 0.5cm 2 The mother species of Cordyceps militaris was inoculated in a 500mL Erlenmeyer flask containing 100mL of special strain culture medium for primary shake flask culture, culture conditions: temperature 20°C, rotation speed 100 rpm, dark culture. The composition and content of the special culture medium for this step are: glucose 20g / L, yeast extract powder 5g / L, MgSO 4 ·7H 2 O 0.5g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, agar 0.5g / L, pH natural.

[0021] (2) the bacterium liquid that step (1) shakes flask to cultivate 5 days is moved into 500mL Erlenmeyer flask and is housed in 100mL special strain culture medium and carry out expansion culture, and inoculum size is 5mL / bottle, is used for producing conidia of Cordyceps militaris; Culture conditions: temperature 20°C, rotation speed 100 rpm, culture in dark. The composition and content of the sp...

Embodiment 2

[0024] The preparation method of Cordyceps militaris liquid strain, its specific steps are:

[0025] (1) Take 0.5cm 2 The mother species of Cordyceps militaris was inoculated in a 500mL Erlenmeyer flask with 100mL of special culture medium for primary shake flask culture, culture conditions: temperature 25°C, rotation speed 50 rpm, dark culture. The composition and content of the special culture medium for this step are: glucose 20g / L, yeast extract powder 5g / L, MgSO 4 ·7H 2 O 0.3g / L, KH 2 PO 4 0.3g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, agar 0.5g / L, pH natural.

[0026](2) Move the bacterium liquid that step (1) shakes flask culture 4 days into 50L fermenter and carry out expansion culture in 30L special-purpose culture medium, the inoculum size is 5% (v / v), and is used for the production of Cordyceps sinensis conidia; culture conditions: temperature 20°C, rotation speed 80 rpm, culture in dark. The composition and content of the special culture medium for this step are: sucr...

Embodiment 3

[0029] The preparation method of Cordyceps militaris liquid strain, its specific steps are:

[0030] (1) Take 0.5cm 2 The mother species of Cordyceps militaris was inoculated in a 500mL Erlenmeyer flask containing 100mL of special culture medium for primary shake flask culture, culture conditions: temperature 18°C, speed 20 rpm, dark culture. The composition and content of the special culture medium for this step are: glucose 20g / L, yeast extract powder 5g / L, MgSO 4 ·7H 2 O 0.3g / L, KH 2 PO 4 0.3g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, agar 0.5g / L, pH natural.

[0031] (2) Move the bacterium solution that step (1) shakes flask culture 7 days into 50L fermenter and carry out expansion culture in 30L special-purpose culture medium, the inoculation amount is 3% (v / v), and is used for the production of northern Cordyceps sinensis conidia; culture conditions: temperature 18°C, rotation speed 50 rpm, culture in dark. The composition and content of the special culture medium for this ...

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Abstract

The invention provides a method for producing the liquid strain of cordyceps militaris. The method comprises the following steps: (1) inoculating the mother culture of cordyceps militaris to a dedicated strain culture medium to carry out the shaking culture; (2) moving the strain solution cultured in step (1) into a dedicated strain culture medium to carry out the amplification culture, wherein the inoculation amount accounts for 2% to 20% of the volume of the culture medium; (3) filtering the strain solution cultured in step (2) by using a sieve with the bore diameter thereof being 50 to 500 meshes to obtain the filtrate containing more than 107 conidia per ml; and (4) diluting the filtrate obtained in step (3) by using sterile water until the density thereof is 105 to 107 conidia per ml to obtain the liquid strain of cordyceps militaris. The method of the invention is applicable to inoculating and cultivating the fruit body of cordyceps militaris, wherein the dedicated strain culture medium contains carbon source, yeast nitrogen base, magnesium sulfate, monopotassium phosphate, dipotassium phosphate and agar.

Description

technical field [0001] The invention relates to a production method of edible fungus cultivation strains, in particular to a method for producing Cordyceps militaris strains through liquid culture, and also relates to the components of these liquid mediums. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris and Cordyceps militaris, belongs to Ascomycotina, Clavcipitaceae and Cordyceps sp. in taxonomy. Cordyceps militaris has similar pharmacological effects to Cordyceps sinensis, and is a rare edible and medicinal fungus. It has been the focus of research and development in the fields of biology, medicine, and health food at home and abroad for many years. At present, the artificial cultivation of fruiting bodies of Cordyceps militaris has been successful. The artificial cultivation of Cordyceps militaris mainly uses liquid strains, which is in line with the development direction of edible fungi. [0003] The use of edible fungus liquid str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 毛先兵朱华李叶敏叶伟
Owner 和润通(重庆)供应链管理有限责任公司
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