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Classical swine fever virus vaccine and production method thereof

A technology of swine fever vaccine and swine fever virus, which is applied in the field of swine fever virus vaccine and its production, can solve the problems that the vaccine production cannot be greatly improved, foreign virus contamination, and low titer of toxin production, etc., and achieve product quality Uniform and stable, low cell inoculum volume, high virus infection efficiency

Inactive Publication Date: 2010-12-29
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Among them, the tissue seedling method and the primary bovine testicular cell seedling method generally have shortcomings such as low yield, low efficiency, foreign virus contamination, low toxin production titer, and large batch-to-batch differences; the cell line method, although it has solved the problem of exogenous virus Pollution problem, the titer of toxin production has also been greatly improved, and the difference between batches has also been reduced, but the vaccine production still cannot be greatly improved, and the process is cumbersome and inefficient, resulting in a lot of waste of personnel

Method used

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  • Classical swine fever virus vaccine and production method thereof
  • Classical swine fever virus vaccine and production method thereof
  • Classical swine fever virus vaccine and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Large-scale production of swine fever virus by tidal type microcarrier suspension bioreactor

[0056] The bioreactor used in this example is a tidal microcarrier bioreactor, and the microcarrier used is polyester fiber. Width 5mm, length 10mm, 1g carrier provides 2,400cm 2 The adherent area can provide 1.0×10 9 The above number of cells grows.

[0057] 1. Viruses and cell lines

[0058] The virus strain used to prepare the CSFV antigen is the attenuated strain of CSFV (purchased from China Veterinary Drug Control Institute). The porcine testis (ST) cell line is used as the cell line for making seedlings, so as to infect and multiply the classical swine fever virus.

[0059] 2 Preparation method

[0060] 1) In a tidal microcarrier bioreactor, add polyester fiber microcarriers, cell growth fluid and porcine testis (ST) cells, at 37 ° C, 5% CO 2 Under the culture environment, start the cell adsorption program to fully combine the microcarriers with the porc...

Embodiment 2

[0078] The comparative test of the swine fever live virus vaccine that embodiment 2 the present invention makes and similar products

[0079] 1. Materials

[0080] (1) Vaccine: 3 batches of swine fever live virus vaccine that embodiment 1 makes, batch number: CSF-T01, CSF-T02, CSF-T03. 1 batch of swine fever live virus vaccine (produced by roller bottle culture system), batch number: CSF-RB01.

[0081] (2) Experimental pigs: select feeding farms or designated pig farms that meet the national experimental animal standards to supply, 8-week-old weaned susceptible piglets, body weight 18-25kg, all negative for classical swine fever antibodies (serum neutralizing antibodies); note Observation before seedlings was carried out for 7 days, once a day in the morning and afternoon, and those with normal body temperature, spirit and appetite were selected for use.

[0082] (3) Attacking virus: CSFV (CSFV) Shimen is a strong strain.

[0083] 2. Method

[0084] (1) Property inspection...

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Abstract

The invention discloses a method for preparing a classical swine fever (CSF) vaccine by using a cell microcarrier suspension culture system, which comprises the following steps of: (1) inoculating cells for preparing the vaccine to a carrier tank containing culture solution and a microcarrier, and uniformly mixing the cells and the microcarrier to make the cells attached to the microcarrier; (2) when the concentration after cell proliferation is 5 to 40 times of the initial inoculation concentration, inoculating CSF virus (lapinized virus) to the cells according to multiplicity of infection (M.O.I.) of the virus of 0.01-1 and reproducing the virus; and (3) mixing prepared virus liquid, adding an appropriate freeze-drying protective agent, fully and uniformly mixing, quantitatively packaging, and freeze-drying to obtain the CSF vaccine. The CSF vaccine produced by the method has the advantages of high density of cultured cells, continuous culture, high yield of the virus, high immune effect, high safety, complete immune protection on attack of violent CSF, and the like.

Description

technical field [0001] The invention belongs to the technical field of biological products, and relates to a swine fever virus vaccine and a production method thereof. Background technique [0002] Classical Swine fever (CSF for short), also known as Hog cholera or European swine fever, is a viral infectious disease with acute or hyperacute fever in pigs, clinically characterized by Rapid dissemination, fever, and typical hemorrhagic lesions can be seen on autopsy. Swine fever can infect pigs of all ages and is prevalent throughout the year with high morbidity and mortality, which is extremely harmful to pigs. This disease is one of the most important infectious diseases threatening the pig industry. The World Organization for Animal Health (OIE) lists swine fever as one of the 16 notifiable infectious diseases of category A, and China defines it as a severe infectious disease. [0003] Compulsory immunization with swine fever live virus vaccine is currently the best way ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/187C12N7/08A61P31/14C12R1/93
Inventor 张许科孙进忠乔荣岑
Owner PU LIKE BIO ENG
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