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Method for preparing directed vat set bacillus coagulans starter

A technology of bacillus coagulans and starter, applied in the field of biological preparations, can solve problems such as unavailable products, achieve the effects of saving labor, preventing bacterial contamination and degradation, and shortening the production cycle

Active Publication Date: 2012-05-02
武汉华联科生物技术有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, there is no product of direct-throwing Bacillus coagulans starter at home and abroad

Method used

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  • Method for preparing directed vat set bacillus coagulans starter
  • Method for preparing directed vat set bacillus coagulans starter
  • Method for preparing directed vat set bacillus coagulans starter

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Embodiment Construction

[0027] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0028] The strain used in the present invention is Bacillus coagulans TQ33, the classification name is Bacillus coagulans, the preservation number is: CGMCC No.5233, the preservation date is September 9, 2011, and the preservation address is: Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing No. 3, depository unit: General Microbiology Center of China Committee for the Collection of Microbial Cultures.

[0029] 1. Seed and fermentation medium:

[0030] Liquid seed medium (g / L): peptone 20.0, yeast extract 10.0, glucose 20.0, CaCO 3 10.0, pH7.2-7.4.

[0031] Liquid fermentation medium (g / L): peptone 10.0, yeast extract 10.0, glucose 6.0, pH 7.0.

[0032] 2. Cell activation:

[0033] The strains frozen in the glycerol tube ...

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Abstract

The invention relates to a method for preparing a directed vat set bacillus coagulans starter. The method comprises the following steps of: concentrating fermentation liquor of bacillus coagulans, wherein the concentration conditions of the fermentation liquor are that: the centrifugal rotating speed is 6,000r / min, the centrifugal time is 20min, and the centrifugal temperature is 4 DEG C; adding a protective agent, wherein the adopted protective agent is an aqueous solution which contains 5 percent m / m sucrose and 10 percent m / m maltodextrin; adding bacterial sludge into the aqueous solution of the protective agent; and drying by a vacuum drying method at the drying temperature of 60 DEG C under the drying pressure of 12,000Pa. The viable count of the obtained starter is 3.24*10<10>cfu / g; the sporation rate reaches 90.3 percent; and ideal liquid and solid fermentation effects can be achieved according to one thousandth of inoculation amount.

Description

technical field [0001] The invention belongs to the field of biological preparations and relates to a preparation method of a direct-throwing Bacillus coagulans starter. Background technique [0002] Bacillus coagulans is a kind of facultative anaerobic bacteria with terminal spore structure, which is a normal microorganism beneficial to the host. It is widely added to various foods and feeds. After eating, it has a strong ability to reproduce and colonize in the body, and has a high number of viable bacteria, which is very beneficial to human health. The health effects of Bacillus coagulans on the human body are mainly through two ways: one is the function of Bacillus coagulans itself, which can produce a large number of beneficial metabolites, inhibit the growth of spoilage bacteria, and maintain the balance of the intestinal flora of the body; Metabolites can promote intestinal peristalsis, enhance enzyme activity, and help the body digest and absorb. [0003] Directed ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/07
Inventor 王海宽戚薇周超
Owner 武汉华联科生物技术有限公司
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