Seed culture medium and culture method for streptomyces aureus

Abstract

The invention relates to a seed culture medium and a culture method for streptomyces aureus. The seed culture medium comprises beer yeast dry residue, and particularly comprises the following raw materials: 8 to 10 ml / L oil, 20 to 22 g / L starch, 27 to 29 g / L beer yeast dry residue, 8 to 9 g / L yeast extract, 11 to 13 g / L low temperature soybean cake powder, 0.3 to 0.4 g / L dipotassium phosphate, 0.8 to 1 g / L light calcium carbonate, 0.04 to 0.06 g / L magnesium sulphate, 0.8 to 0.9 g / L sodium chloride, 0.2 to 0.3 g / L amylase, 0.05 to 0.07 g / L ammonium sulfate and 0.03 to 0.04 g / L polyether antifoaming agents. The seed culture medium and the culture method can effectively improve the bacterial cell consistence and bacterial cell culture quality of streptomyces aureus, reduce the inoculum dose in the initial stage of the seed culturing, shorten the seed culturing period, meanwhile reduce the raw auxiliary material dose in the seed culturing to the maximum extent, reduce the production cost, ensure the abundant supply, and realize the stability and high quality culturing of the streptomyces aureus.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, in particular to a seed culture medium and a culture method for reducing the inoculum amount of Streptomyces aureus by using beer yeast dry residue. Background technique [0002] Streptomyces aureus is a type of Streptomyces, which is named for its golden color. Streptomyces aureus has a strong ability to synthesize aureomycin when the temperature is below 30°C; when the temperature exceeds 35°C, it only synthesizes tetracycline. [0003] At present, the organic nitrogen sources commonly used in the seed medium for cultivating Streptomyces aureus in China are mainly corn gluten meal, peanut cake powder, yeast powder and peptone. The main problems that exist: [0004] 1) There are many types of organic nitrogen sources in the formula of the seed medium, and more than 4 kinds of organic nitrogen sources need to be added, which increases the purchase cost of the production enterpri...

AI Technical Summary

Problems solved by technology

[0004] 1) There are many kinds of organic nitrogen sources in the formula of the seed medium, and more than 4 kinds of organic nitrogen sources need to be added, which increases the purchase cost of the production enterprise and the seed cultivation cost

[0005]2) The seed cultivation time is longer, usually 24-28 hours

[0006]3) In the process of seed cultivation, the quality of the organic nitrogen source is likely to affect the shape and concentration of the bacteria, resulting in a decrease in the quality of the seed solution

[0007]4) Before cultivating Streptomyces aureus seeds, the inoculation amount of the seed culture medium is relatively large, generally 600-800ml / m3, increasing reduce the workload of strain researchers