Seed culture medium and culture method for streptomyces aureus

A technology of Streptomyces aureus and a culture method, applied in the field of biological fermentation, can solve the problems of increasing the procurement cost and seed cultivation cost of production enterprises, increasing the workload of bacteria researchers, easily affecting the shape and concentration of bacteria, and reducing procurement costs. and production costs, reduction in dosage and species, and the effect of reduction in inoculum

Active Publication Date: 2014-01-01
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1) There are many kinds of organic nitrogen sources in the formula of the seed medium, and more than 4 kinds of organic nitrogen sources need to be added, which increases the purchase cost of the production enterprise and the seed cultivation cost
[0005]2) The seed cultivation time is longer, usually 24-28 hours
[0006]3) In the process of seed cultivation, the quality of the organic nitrogen source is likely to affect the shape and concentration of the bacteria, resulting in a decrease in the quality of the seed solution
[0007]4) Before cultivating Streptomyces aureus seeds, the inoculation amount of the seed culture medium is relatively large, generally 600-800ml / m3, increasing reduce the workload of strain researchers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Configure seed medium 5m 3 : Soybean oil 40L, corn starch 105kg, beer yeast dry residue 145kg, yeast paste 40kg, low-temperature soybean cake powder 60kg, dipotassium hydrogen phosphate 2.0kg, light calcium carbonate 4kg, magnesium sulfate 0.25kg, sodium chloride 4.5kg, amylase 1kg, ammonium sulfate 0.3kg, silicone oil defoamer 200ml.

[0033] The quality of beer yeast dry residue: protein content is 54.3%, phosphorus content is 62.3ug / ml, water content is 4.8%, ash content is 4.1%, and carbohydrate content is 13.7%.

[0034] The seed medium is sterilized, cooled, and pressurized with sterile air. The sterilized seed culture medium has no bacteria contamination, and the sterilized pH is 6.2. Then under flame protection, insert the Streptomyces aureus that has cultivated into the seed tank and carry out seed culture, and the inoculum size is 900ml.

[0035] During the seed cultivation process, the tank pressure is 0.02MPa; 10h before fermentation, the tank tempera...

Embodiment 2

[0037] Configure seed medium 5m 3 : Corn oil 45L, wheat starch 110kg, beer yeast dry residue 135kg, yeast paste 42.5kg, low-temperature soybean cake powder 65kg, dipotassium hydrogen phosphate 1.5kg, light calcium carbonate 4.5kg, magnesium sulfate 0.3kg, sodium chloride 4.0kg , Amylase 1.25kg, ammonium sulfate 0.35kg, silicone oil defoamer 150ml.

[0038] The quality of beer yeast dry residue: protein content is 52.1%, phosphorus content is 68.6ug / ml, water content is 4.2%, ash content is 4.5%, and carbohydrate content is 14.2%.

[0039] The seed medium is sterilized, cooled, and pressurized with sterile air. The sterilized seed medium was free of bacteria contamination, and the sterilized pH was 6.3. Then under flame protection, insert the Streptomyces aureus that has cultivated into the seed tank and carry out seed culture, and the inoculum size is 920ml.

[0040] During the seed cultivation process, the tank pressure is 0.03MPa; 10h before fermentation, the tank t...

Embodiment 3

[0042] Configure seed medium 5m 3 , rapeseed oil 50L, sweet potato starch 100kg, beer yeast dry residue 140kg, yeast paste 45kg, low-temperature soybean cake powder 55kg, dipotassium hydrogen phosphate 1.75kg, light calcium carbonate 5kg, magnesium sulfate 0.2kg, sodium chloride 4.25kg, Amylase 1.5kg, ammonium sulfate 0.25kg, silicone oil defoamer 175ml.

[0043] The quality of beer yeast dry residue: protein content is 56.1%, phosphorus content is 71.4ug / ml, water content is 4.0%, ash content is 4.4%, and carbohydrate content is 14.8%.

[0044] The seed medium is sterilized, cooled, and pressurized with sterile air. The sterilized seed culture medium has no bacteria contamination, and the sterilized pH is 6.4. Then under flame protection, insert the Streptomyces aureus that has cultivated into the seed tank and carry out seed culture, and the inoculum size is 950ml.

[0045] During the seed cultivation process, the tank pressure is 0.04MPa; 10h before fermentation, the tan...

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PUM

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Abstract

The invention relates to a seed culture medium and a culture method for streptomyces aureus. The seed culture medium comprises beer yeast dry residue, and particularly comprises the following raw materials: 8 to 10 ml / L oil, 20 to 22 g / L starch, 27 to 29 g / L beer yeast dry residue, 8 to 9 g / L yeast extract, 11 to 13 g / L low temperature soybean cake powder, 0.3 to 0.4 g / L dipotassium phosphate, 0.8 to 1 g / L light calcium carbonate, 0.04 to 0.06 g / L magnesium sulphate, 0.8 to 0.9 g / L sodium chloride, 0.2 to 0.3 g / L amylase, 0.05 to 0.07 g / L ammonium sulfate and 0.03 to 0.04 g / L polyether antifoaming agents. The seed culture medium and the culture method can effectively improve the bacterial cell consistence and bacterial cell culture quality of streptomyces aureus, reduce the inoculum dose in the initial stage of the seed culturing, shorten the seed culturing period, meanwhile reduce the raw auxiliary material dose in the seed culturing to the maximum extent, reduce the production cost, ensure the abundant supply, and realize the stability and high quality culturing of the streptomyces aureus.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, in particular to a seed culture medium and a culture method for reducing the inoculum amount of Streptomyces aureus by using beer yeast dry residue. Background technique [0002] Streptomyces aureus is a type of Streptomyces, which is named for its golden color. Streptomyces aureus has a strong ability to synthesize aureomycin when the temperature is below 30°C; when the temperature exceeds 35°C, it only synthesizes tetracycline. [0003] At present, the organic nitrogen sources commonly used in the seed medium for cultivating Streptomyces aureus in China are mainly corn gluten meal, peanut cake powder, yeast powder and peptone. The main problems that exist: [0004] 1) There are many types of organic nitrogen sources in the formula of the seed medium, and more than 4 kinds of organic nitrogen sources need to be added, which increases the purchase cost of the production enterpri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/49
Inventor 王义任勇
Owner 宁夏泰瑞制药股份有限公司
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