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Culture medium for fermentation production of spectinomycin through streptomyces spectabilis and fermentation method

A technology of Streptomyces spectiosa and fermentation medium, which is applied in the medium and fermentation field of Streptomyces spectinomycin to produce Spectinomycin. Purchasing and production costs, reducing usage and variety, and improving work efficiency

Active Publication Date: 2014-01-01
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1 Spectinomycin seeds and the composition of organic nitrogen sources in the fermentation medium are at least three, which increases the purchase cost of the enterprise
[0005] 2 The first-level seed inoculation amount is more
The inoculum volume of conventional primary seed culture is 5-10L / M 3 , increasing the work intensity of strain researchers
[0006] 3 The seed culture cycle of spectinomycin with conventional formula is longer, and the cycle of two seed cultures exceeds 50h
In addition, the price of purchasing pure fishmeal is relatively high, and it is easily affected by the fishing moratorium, which increases the purchase cost and production cost
[0008] 5 During the sterilization process of conventional medium, part of the glucose is easily carbonized at high temperature, which makes the color of seed liquid and fermentation liquid darker, reduces the total sugar content in the medium, and affects the effect of seed culture and fermentation culture

Method used

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  • Culture medium for fermentation production of spectinomycin through streptomyces spectabilis and fermentation method
  • Culture medium for fermentation production of spectinomycin through streptomyces spectabilis and fermentation method
  • Culture medium for fermentation production of spectinomycin through streptomyces spectabilis and fermentation method

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Experimental program
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Effect test

Embodiment 1

[0072] Quality of beer yeast dry residue: protein 52.3%; phosphorus 96ug / ml; moisture 4.1%; ash content 3.9%; carbohydrate 14.8%;

[0073] Earthworm powder quality: protein 64.2%; water 8.4%; all passed through 80-mesh sieve.

[0074] Configure primary seed medium 1m 3 . The composition of the primary seed medium is: corn oil 18L, maltose 5kg, beer yeast dry residue 13kg, earthworm powder 17kg, ammonium sulfate 0.05kg, maltase 0.04kg, polyether defoamer 0.03kg.

[0075] Configure secondary seed medium 10m 3 . The composition of the secondary seed medium is: 200L corn oil, 70kg maltose, 180kg beer yeast dry residue, 180kg earthworm powder, 0.4kg ammonium sulfate, 0.6kg maltase, and 0.1kg polyether defoamer.

[0076] Configure fermentation medium 100m 3 . The composition of the fermentation medium is: corn oil 2300L, maltose 2300kg, beer yeast dry residue 2300kg, earthworm powder 2100kg, ammonium sulfate 7kg, potassium dihydrogen phosphate 4kg, potassium chloride 4kg, ligh...

Embodiment 2

[0082] Quality of beer yeast dry residue: protein 53.7%; phosphorus 89.7ug / ml; moisture 3.8%; ash content 3.7%; carbohydrate 13.5%;

[0083] Earthworm powder quality: protein 65.9%; moisture 7.8%; all passed through 80-mesh sieve.

[0084] Configure primary seed medium 1m 3 . The composition of the primary seed medium is: corn oil 19L, maltose 4kg, beer yeast dry residue 12kg, earthworm powder 19kg, ammonium sulfate 0.03kg, maltase 0.05kg, polyether defoamer 0.01kg.

[0085] Configure secondary seed medium 10m 3 . The composition of the secondary seed medium is: 210L corn oil, 60kg maltose, 170kg beer yeast dry residue, 200kg earthworm powder, 0.5kg ammonium sulfate, 0.7kg maltase, and 0.2kg polyether defoamer.

[0086] Configure fermentation medium 100m 3 . The composition of the fermentation medium is: corn oil 2400L, maltose 2200kg, beer yeast dry residue 2200kg, earthworm powder 2300kg, ammonium sulfate 8kg, potassium dihydrogen phosphate 4kg, potassium chloride 3kg,...

Embodiment 3

[0092] Quality of beer yeast dry residue: protein 56.6%; phosphorus 92.3ug / ml; moisture 3.9%; ash content 4.5%; carbohydrate 15.1%;

[0093] Earthworm powder quality: protein 62.9%; moisture 8.8%; all passed through 80-mesh sieve.

[0094] Configure primary seed medium 1m 3 . The composition of the primary seed medium is: 20L of corn oil, 3kg of maltose, 14kg of beer yeast dry residue, 18kg of earthworm powder, 0.04kg of ammonium sulfate, 0.06kg of maltase, and 0.02kg of polyether defoamer.

[0095] Configure secondary seed medium 10m 3 . The composition of the secondary seed medium is: 220L corn oil, 5kg maltose, 190kg beer yeast dry residue, 190kg earthworm powder, 0.6kg ammonium sulfate, 0.5kg maltase, and 0.3kg polyether defoamer.

[0096] Configure fermentation medium 100m 3 . The composition of the fermentation medium is: corn oil 2500L, maltose 2100kg, beer yeast dry residue 2400kg, earthworm powder 2200kg, ammonium sulfate 8kg, potassium dihydrogen phosphate 3kg,...

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Abstract

The invention relates to a culture medium for fermentation production of spectinomycin through streptomyces spectabilis and a fermentation method. A primary seed culture medium, a secondary seed culture medium and a fermentation culture medium all contain corn oil, maltose, beer yeast dry residues and earthworm meal. According to the invention, maltose replaces glucose, beer yeast dry residues and earthworm meal replace fish meal, peptone, soybean cake meal and corn steep liquor, and the culture medium formula is optimized, so that the problem of high costs of raw materials is solved, the environmental effect on the source of the raw materials is reduced to the utmost extent, sufficient supply of the raw materials is guaranteed, and stable and efficient production of spectinomycin is realized. Meanwhile, the culture medium can improve the fermentation unit and shorten the fermentation period.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a culture medium and a fermentation method for producing spectinomycin by fermentation of Streptomyces spectiformis. Background technique [0002] Spectinomycin is a secondary metabolite produced by Streptomyces spectiosa through aerobic fermentation. It has the characteristics of broad bactericidal spectrum, no allergies, few side effects, and good safety. It is not only the first choice drug for treating gonorrhea, but also can be used for animal treatment, and can promote animal growth, and is a good animal feed additive. [0003] At present, the production method of spectinomycin in China is mainly the fermentation method, that is, Streptomyces spectiformis is used as the strain, and the carbon source in the medium is mainly starch and glucose; the organic nitrogen source is mainly corn steep liquor, yeast powder, soybean cake powder and Peptone. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12R1/465
Inventor 任勇王义奇乃
Owner 宁夏泰瑞制药股份有限公司
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