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Vaccine produced by suspended microcarrier cell culture system and method for producing vaccine

A cell culture and microcarrier technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the huge cost of space and equipment use and maintenance, increase the burden of human labor costs, and cannot culture cells in large quantities at one time, etc. problems, to achieve the effect of saving costs and manpower, easy control, and high production efficiency

Active Publication Date: 2010-10-27
香港维克贸易有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the disadvantages of this traditional cell culture process are: the preparation time is cumbersome, the cells cannot be cultured in large quantities at one time, and each culture plate or culture flask can only culture a single layer of cells
If you want to cultivate a large number of people in a short period of time, the required space and equipment use and maintenance costs are very large, which will increase the cost burden of manpower and material resources for enterprises

Method used

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  • Vaccine produced by suspended microcarrier cell culture system and method for producing vaccine
  • Vaccine produced by suspended microcarrier cell culture system and method for producing vaccine
  • Vaccine produced by suspended microcarrier cell culture system and method for producing vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The making of embodiment 1 bird flu live virus vaccine

[0031] 1.1 Viruses and cell lines

[0032] The virus used to make avian influenza vaccine must be provided by a government-authorized unit. It is the H5N1 reassortant vaccine prototype strain (reassortant), which is isolated from a highly pathogenic avian influenza virus strain (for example: A / Hong Kong / 213 / 2003 or A / VietNam / 1194 / 2004, or NIBRG-14). After the pathogenicity test, the results show that the H5N1 reassortment vaccine prototype strain virus is not pathogenic before it can be used to make H5N1 avian influenza vaccine. In addition, a cell line with good susceptibility to the virus strain (the dog kidney cell line Madin Darby Canine Kidney, MDCK was used in this embodiment) was used as the cell line for seedling production to infect and multiply the H5N1 avian influenza virus in large quantities.

[0033] Cell lines, seed strains, and virus strains for mass production are all in accordance with the pro...

Embodiment 2

[0052] 2.1 Preparation of porcine pseudorabies live virus vaccine

[0053] 2.1.1 Viruses and cell lines

[0054] The virus used to make the porcine pseudorabies live virus vaccine is obtained by removing the gI glycoprotein and thymidine kinase (TK) double genes on the virulent strain (LC) of pseudorabies virus (Pseudorabies virus, PRV) by genetic engineering. Attenuated strains with gene defects. After the pathogenicity test, the result shows that gI glycoprotein and thymidine kinase (TK) double gene defect attenuated strain (LCM strain) virus has no pathogenicity, and it can be used to make porcine pseudorabies live virus vaccine. And use the cell line that has good susceptibility to this virus strain (the present embodiment selects hamster young mouse kidney cell BHK cell for use; Other cells such as: rabbit kidney cell (PK13) or African green monkey kidney cell (VERO) are also to swine pseudorabies Porcine pseudorabies virus has good susceptibility), as a cell line for s...

Embodiment 3

[0110] 3.1 Production of live Japanese encephalitis vaccine

[0111] 3.1.1 Viruses and cell lines

[0112] The virus used to make Japanese encephalitis live virus vaccine is the "at" strain, which is collected from pigs infected with Japanese encephalitis and passed 220 generations on hamster kidney cells (HK cells) to become Attenuated strain. After the pathogenicity test, the results show that the attenuated strain of the virus is not pathogenic before it can be used to make Japanese encephalitis live virus vaccine. And use the cell line that this virus strain has good susceptibility (the present embodiment selects hamster kidney cell (HK); Other cells such as: pig kidney cell (PK) and African green monkey kidney cell (VERO) are also to Japanese encephalitis virus have good susceptibility), as a cell line for making seedlings, by infecting and multiplying Japanese encephalitis virus in large quantities.

[0113] 3.1.2 Method

[0114] (1) Inoculate HK cells for seedling p...

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Abstract

The invention discloses a vaccine produced by a suspended microcarrier cell culture system and a method for producing the vaccine. The method comprises the following technical steps of: (1) inoculating cells for preparing the vaccine to a culture tank which contains a culture medium and a microcarrier; (2) uniformly mixing the cells and the microcarrier to make the cells attached to the microcarrier; (3) providing sufficient nutrient and gas for the cells at an appropriate temperature to make the cells continue growing on the microcarrier; (4) preparing virus suspension from viruses for preparing the vaccine, inoculating the virus suspension to the cells and continuing culturing, and harvesting virus liquid or the cells containing the viruses and replacing culture solution at intervals of1 to 3 days; and (5) after purifying the harvested virus liquid, inactivating the virus liquid as required, adding a proper adjuvant into the inactivated virus liquid, adding a proper freeze-drying protective agent into activated virus liquid, and quantitatively packaging after fully and uniformly mixing to obtain the vaccine. The method has the advantages of simple production process and capability of obviously improving the yield and quality of the vaccine.

Description

technical field [0001] The invention belongs to the technical field of biological products, and more specifically relates to a vaccine produced by a suspension microcarrier cell culture system and a method thereof. Background technique [0002] Currently known production methods for whole virus vaccines mostly use chicken embryo eggs, animal tissue or cell culture to produce virus; The production of whole-virus vaccines contains too many foreign proteins, which may easily cause allergic reactions and neurological complications in the recipients; therefore, a better way is to find cells that are sensitive to the virus and use cell culture to expand the vaccine. mass-produced viruses. [0003] Adhesive cell culture is used to produce viruses to manufacture whole virus vaccines (including live virus and inactivated vaccines), and mass culture of cells is one of the keys to vaccine production. Traditionally, cell lines (adherent) are used for vaccine production. Cultivate, fir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/145A61K39/12A61K39/205A61P31/14A61P31/16C12N7/00C12R1/93
Inventor 郭村勇陈旭中
Owner 香港维克贸易有限公司
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