Method for rapidly and selectively breeding terramycin strains

An oxytetracycline and strain technology, which is applied in the field of oxytetracycline strain selection and breeding, can solve problems such as affecting work efficiency and long screening period, and achieve the effects of improving work efficiency and shortening verification time.

Inactive Publication Date: 2015-05-13
HEBEI SHENGXUE DACHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the technical problem that the screening cycle of oxytetracycline industrial strains in the prior art is long and affects work efficiency, the present invention provides a method for rapid breeding of oxytetracycline strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The composition of the test tube slant culture medium according to mass percentage is: 6.0%-7.0% bran, 2.0%-2.5% agar.

[0026] The composition of the separation medium by mass percentage is: 2.0%-3.0% cornstarch, 0.2%-0.5% corn steep liquor, 0.1%-0.3% ammonium sulfate, 0.1%-0.5% calcium carbonate, 0.3%-0.5% sodium chloride , 2.0%-2.5% agar.

[0027] The composition of the fermentation medium according to mass percentage is: 4%-5% cornstarch, 0.1%-0.2% corn steep liquor, 0.5%-1.0% soybean powder, 0.2%-0.3% ammonium sulfate, 0.2%-0.3% calcium carbonate, 0.1%-0.2% sodium chloride, 0.01%-0.02% potassium dihydrogen phosphate.

[0028] a. test tube slant culture: inoculate the oxytetracycline production strain sandy soil spores in the test tube slant culture medium, the sandy soil spore inoculation amount of each test tube slant is, cultivate 5 under the condition of temperature 37.0 ± 0.5 ℃, humidity 40%. days, get slant spores;

[0029] b. Preparation of spore suspensio...

Embodiment 2

[0038] The composition of the test tube slant culture medium according to mass percentage is: 6.0%-7.0% bran, 2.0%-2.5% agar.

[0039] The composition of the separation medium by mass percentage is: 2.0%-3.0% cornstarch, 0.2%-0.5% corn steep liquor, 0.1%-0.3% ammonium sulfate, 0.1%-0.5% calcium carbonate, 0.3%-0.5% sodium chloride , 2.0%-2.5% agar.

[0040] The composition of the fermentation medium according to mass percentage is: 4%-5% cornstarch, 0.1%-0.2% corn steep liquor, 0.5%-1.0% soybean powder, 0.2%-0.3% ammonium sulfate, 0.2%-0.3% calcium carbonate, 0.1%-0.2% sodium chloride, 0.01%-0.02% potassium dihydrogen phosphate.

[0041] a. test tube slant culture: inoculate the sand spores of the oxytetracycline production strain in the test tube slant medium, the inoculation amount of the sand spores on the slant of each test tube is 37.0 ± 0.5 ℃ at temperature, and cultivate under the condition of humidity 60% for 3 days, get slant spores;

[0042] b. Preparation of spor...

Embodiment 3

[0051] The composition of the test tube slant culture medium according to mass percentage is: 6.0%-7.0% bran, 2.0%-2.5% agar.

[0052] The composition of the separation medium by mass percentage is: 2.0%-3.0% cornstarch, 0.2%-0.5% corn steep liquor, 0.1%-0.3% ammonium sulfate, 0.1%-0.5% calcium carbonate, 0.3%-0.5% sodium chloride , 2.0%-2.5% agar.

[0053] The composition of the fermentation medium according to mass percentage is: 4%-5% cornstarch, 0.1%-0.2% corn steep liquor, 0.5%-1.0% soybean powder, 0.2%-0.3% ammonium sulfate, 0.2%-0.3% calcium carbonate, 0.1%-0.2% sodium chloride, 0.01%-0.02% potassium dihydrogen phosphate.

[0054] a. Test tube slant culture: inoculate the sandy soil spores of the oxytetracycline production strain in the test tube slant medium, the inoculum amount of the sandy soil spores on each test tube slant is, cultivate 4.5% under the conditions of temperature 37.0±0.5°C and humidity 45%. days, get slant spores;

[0055] b. Preparation of spore ...

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Abstract

The invention relates to a method for rapidly and selectively breeding terramycin strains. The method comprises the following steps: a, test tube slant culture; b, preparation of spore suspension; c, isolated culture; d, fermentation culture; e, strain re-testing. Single colonies obtained through isolated culture are directly inoculated into a fermentation medium for fermentation culture, so that the titer of the terramycin obtained through screening is high, the titer validation time of the single colonies is shortened to 48-50h, and the working efficiency is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of breeding oxytetracycline strains, and in particular relates to a method for rapid breeding of oxytetracycline strains. Background technique [0002] Oxytetracycline (Spectniomycni) is a secondary metabolite produced by Streptomyces rimosus after aerobic fermentation. It has antibacterial effect on a variety of cocci and bacilli, and also has inhibitory effect on Rickettsia and amoeba, and is used to treat upper respiratory tract infection, gastrointestinal infection, typhus, scrub typhus, mycoplasma and chlamydia Is an infection and other diseases. [0003] Oxytetracycline is mainly used as medicine and feed additive for livestock and poultry. In addition, the market prospect of producing semi-synthetic antibiotics with oxytetracycline as raw material is good. For example, doxycycline (doxycycline) synthesized with it has a market price of Oxytetracycline is 5-7 times that of oxytetracycline, and the cl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/59
CPCC12N1/20
Inventor 张慧赵丽红
Owner HEBEI SHENGXUE DACHENG PHARMA
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