32results about How to "Increase the wall area" patented technology

The invention discloses a method for producing animal rabies viruses and animal rabies vaccines on a large scale. By using a bioreactor, the animal rabies viruses and the animal rabies vaccines are produced by a cell micro-carrier suspension culturing system on a large scale by the following steps of: inoculating cells for preparing the vaccine into a carrier tank containing culture solution and micro-carriers to attach the cells to the micro-carriers; growing the cells on the micro-carriers until the concentration of culture solution is 5 to 20 times the inoculation concentration under a proper culturing environment; preparing virus suspension from the rabies virus to adsorb the virus suspension to the cells; replacing cell maintenance culture solution to culture the virus under the proper culturing environment; continuously culturing for 3 to 5 days and harvesting the virus solution for the first time, wherein the solution replacement ratio is 50 percent by using a semi-continuous process; continuously culturing for 9 to 11 days and harvesting and replacing the solution once every 24 hours; and mixing the harvested virus solution and the virus solution of the bioreactor, repeatedly performing freeze-thawing twice at the constant temperature of -20 DEG C, inactivating, purifying and adding adjuvants to prepare the rabies vaccine. The method has the advantages of large production scale, high single-batch yield and relatively low production cost.

The invention relates to a method for preparing porcine parvovirus inactivated vaccines, and belongs to the field of biotechnology. The method for preparing the porcine parvovirus inactivated vaccines includes the following steps: (1) culturing of cells used for vaccine preparation, (2) virus inoculation and culture, (3) virus liquid harvest, (4) virus liquid inactivation, and (5) vaccine preparation. Seed virus used for vaccine preparation and cells are screened, matching degree between the virus and the cells is strengthened, a riptide perfusion type bioreactor culture system is used for improving multiplication titer and harvest yield of the virus, immune effects are improved through the improvement of an emulsification process and a whole production process does not involve other biosafety and public health problems and is suitable for large scale production.

The invention discloses a method for preparing a porcine circovirus 2-type inactivated vaccine, and belongs to the technical field of organisms. The method comprises the following steps of: a, cultivation of cells for seeding; b, virus inoculation and cultivation; c, virus liquid harvesting; d, virus liquid inactivation; and e, vaccine preparation. By adopting the method, the seeding cells are screened; the matching property of the cells and the virus is enhanced; the multiplication titer and the harvesting yield of the virus are improved by adopting a torrent perfusion-type bioreactor culture system; the immune effect is improved by improvement of an emulsifying process; the entire production process is not involved with other problems of biosafety and public health; and the method is suitable for mass production.

The invention provides an on-site real-time treatment system for laboratory organic waste liquid, wherein the waste liquid reaction device (1) includes a primary reactant (11) and a secondary reactant (12) communicated through channels; the primary reactant ( 11) It is olive-shaped, with a flame stabilizer (8) inside and primary air and waste liquid; the secondary reaction body (12) is in the shape of a straight cylinder with eccentric necking, the left side of the top is a slope and the right side is a vertical surface, and the top The outlet section is about 1 / 2-2 / 3 of the middle section; the reactant of the waste liquid reaction device (1) is cooled by the waste heat recovery device (3), quenched and desulfurized by the flue gas quenching and desulfurization integrated device (4), and dehydrated The device (5) removes moisture, and finally the harmful components are filtered out by the flue gas purification device (6). The first-stage reaction body can guarantee the reaction space of the waste liquid; the second-stage reaction body can ensure the waste liquid treatment temperature; the invention is beneficial to the staged treatment of the waste liquid, increases the reaction process of the reactants, and ensures the reaction temperature, space and time of the waste liquid.

The invention discloses a human placenta chorionic mesenchymal stem cell separation method, which comprises: 1) taking healthy human placenta chorionic tissue, cutting into strip blocks, and completely rinsing with physiological saline; 2) adding a proper amount of physiological saline, chopping the chorion into fine particles by using a homogenizer, and rinsing with physiological saline until the solution is clarified; 3) treating again with the homogenizer to achieve micro-particles, and carrying out centrifugation to remove the upper layer blood cells; 4) adding an enzyme to the precipitate, digesting, carrying out centrifugation, and separating the precipitate; 5) carrying out centrifugal washing with physiological saline twice, adding a culture medium to the precipitate, inoculating, and culturing; and 6) discarding the tissue blocks and the culture medium at the 7th day, rinsing the bottom of the culture flask by using physiological saline, adding a fresh culture medium, changing the culture medium every 3 days until the cell fusion degree achieves 80-90%, and carrying out digestion passage with trypsin to obtain the human placenta chorionic mesenchymal stem cells. According to the present invention, the operation is simple and rapid, and the high-quality human placenta chorionic mesenchymal stem cells can be obtained.

Provided a high-pressure homogenizer comprising a channel module comprising a microchannel through which an object for homogenization passes, wherein the microchannel is provided with a first flow channel and a second flow channel sequentially arranged along the direction through which the object passes, the first flow channel is provided with a plurality of first baffles disposed so as to partition the microchannel into a plurality of spaces, the second flow channel is provided with a plurality of second baffles disposed so as to partition the microchannel into a plurality of spaces, and at least one of the first baffles is provided to be positioned between two adjacent second baffles.