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32results about How to "Increase the wall area" patented technology

Animal rabies virus and vaccine and production method thereof

The invention discloses a method for producing animal rabies viruses and animal rabies vaccines on a large scale. By using a bioreactor, the animal rabies viruses and the animal rabies vaccines are produced by a cell micro-carrier suspension culturing system on a large scale by the following steps of: inoculating cells for preparing the vaccine into a carrier tank containing culture solution and micro-carriers to attach the cells to the micro-carriers; growing the cells on the micro-carriers until the concentration of culture solution is 5 to 20 times the inoculation concentration under a proper culturing environment; preparing virus suspension from the rabies virus to adsorb the virus suspension to the cells; replacing cell maintenance culture solution to culture the virus under the proper culturing environment; continuously culturing for 3 to 5 days and harvesting the virus solution for the first time, wherein the solution replacement ratio is 50 percent by using a semi-continuous process; continuously culturing for 9 to 11 days and harvesting and replacing the solution once every 24 hours; and mixing the harvested virus solution and the virus solution of the bioreactor, repeatedly performing freeze-thawing twice at the constant temperature of -20 DEG C, inactivating, purifying and adding adjuvants to prepare the rabies vaccine. The method has the advantages of large production scale, high single-batch yield and relatively low production cost.
Owner:PULIKE BIOLOGICAL ENG INC

Virus and vaccine of porcine reproductive and respiratory syndrome and preparation method of same

The invention discloses a method for preparing virus of porcine reproductive and respiratory syndrome on a large scale. In the method, the virus of the porcine reproductive and respiratory syndrome is prepared in a cell microcarrier suspension culture system by a bioreactor. The method comprises the following steps of: inoculating host cells for preparing the virus to a carrier tank containing culture solution and a microcarrier, and mixing the cells and the microcarrier uniformly to ensure that the cells are attached to the microcarrier; providing sufficient nutrients and appropriate gas environment for the cells under the appropriate culture environment to ensure that the cells are grown until the cells are in an amount which are 10 to 20 times of the inoculation concentration on the microcarrier; preparing virus suspension from the virus of the porcine reproductive and respiratory syndrome by using cell maintenance culture solution to ensure that the suspension is adsorbed to the cells; culturing the virus under the appropriate culture environment; culturing continuously for 2 to 3 days to obtain virus solution; and after the virus solution passes inspection, performing freeze thawing on the virus solution twice at the temperature of -20 DEG C, and inactivating and purifying to prepare an inactivated vaccine of the porcine reproductive and respiratory syndrome or adding a freeze-drying protective agent for freeze drying to prepare a live vaccine of the porcine reproductive and respiratory syndrome. The method has large production scale, high yield of single batch and low production cost.
Owner:PU LIKE BIO ENG

Method for producing rabies virus antigens for animals at a large scale

The invention discloses a method for producing rabies virus antigens for animals at a large scale, which produces the rabies virus antigens at a large scale by utilizing a bioreactor by a cell microcarrier suspension culture system. The method comprises the following steps: inoculating cells for preparing the antigens into a carrier tank containing a culture solution and microcarriers to enable the cells to be attached to the microcarriers; in a proper culture environment, enabling the cells to grow on the microcarriers until the quantity of the cells is 5-40 times more than inoculum density; making rabies viruses into a virus suspension, and enabling the virus suspension to be adsorbed on the cells; culturing the viruses in the proper culture environment by using a cell maintenance culture solution; continuously culturing for 3-5 days and then harvesting a virus solution for the first time, wherein a semicontinuous process is adopted and the ratio of a changed solution is 50 percent; continue culturing for 9-11 days, and harvesting the changed solution once every 24 hours; mixing the harvested virus solution with the virus solution of the bioreactor; and carrying out freeze thawing at the temperature of -20 DEG C and inactivation purification to obtain the rabies virus antigens. The method has large production scale, high single-scale yield and relatively low production cost.
Owner:PU LIKE BIO ENG

Centralized and real-time processing system for laboratory waste

The invention relates to a centralized and real-time processing system for laboratory waste. A waste liquid neutralizing device is used for neutralizing waste liquid according to the monitored pH value of the waste liquid; a waste liquid caching device is connected with the waste liquid neutralizing device; a premixing device is connected with the waste liquid caching device, an atomizer is arranged on the premixing device, and a primary wind hole for introducing air is formed in the premixing device; a first-stage reaction device is used for carrying out evaporation and cleavage reaction on awaste gas-liquid-solid mixture conveyed from the premixing device; a micro-particle collection device is used for collecting solid micro-particles separated from the first-stage reaction device; other waste gas-liquid mixtures are collected into a second-stage reaction device from an outlet of the micro-particle collection device; a temperature monitoring point and a secondary wind pipe for introducing hot air are distributed on the second-stage reaction device; a signal end of the temperature monitoring point is connected with a control end of an adjusting valve of a secondary wind supply device; and a purifying device is used for purifying secondary-stage products separated from the second-stage reaction device. The centralized and real-time processing system is high in integration level, compact in structure, low in single amount processing cost of waste and high in processing efficiency.
Owner:上海即崇环保科技有限公司

Simple mobile intelligent illumination plant wall device based on internet of things and control method

The invention belongs to the technical field of intelligent internet-of-things plant walls, and discloses a simple mobile intelligent illumination plant wall device based on internet of things, and acontrol method. After relevant information of a power supply module, a semi-automatic water and fertilizer system and a planting module is acquired by signal sensors which are uniformly distributed ina plant wall support frame, a system controller processes the data, and the working stage of a fan, a water tank and an intelligent illumination module is displayed on a display and controlled. Whenthe humidity and temperature are not normal, an alarm gives out sound, a flashing lamp is turned on to give an alarm, and alarm signals are transmitted to a mobile terminal device through a bluetoothmodule or an internet of things gateway. The invention provides the novel intelligent plant wall capable of remotely acquiring the plant wall information at any time and any place and controlling theworking state of the plant wall. Wall bodies with different sizes can be formed by splicing, the whole system is designed by adopting modularization, and the plant wall device is quite convenient to mount and dismount. In addition, the plant wall can be also moved, so that indoor placement and layout are convenient.
Owner:HUAZHONG UNIV OF SCI & TECH

Attached cell culturing container suitable for automatic equipment

The invention relates to the field of culturing containers, in particular to an attached cell culturing container suitable for automatic equipment. The container comprises a culturing container bottlebody, a fully air-permeable bottle cap and a cell attaching wall panel, the culturing container bottle body comprises a centrifugal bottle bottom collector, a bottle mouth, an inner cell attaching wall, an oblique slope panel and a cell attaching wall panel, the interior of the centrifugal bottle bottom collector is provided with an oblique slope panel, one end of the centrifugal bottle bottom collector is provided with an inner cell attaching wall, the interior of the inner cell attaching wall comprises a plurality of cell attaching wall panels, and a scaling ruler is arranged at the exterior of the culturing container bottle body. The container has the advantages that the container can be used as a single container in the attached cell culturing process for culturing, centrifugation, sample observation, in-process transferring and the like, liquid bottle opening / closing operation, liquid transferring operation, multi-type container storage and multi-type container conversion in thewhole automatic production process are simplified, and the production efficiency and benefits are effectively improved.
Owner:INNOVEL INTELLIGENT TECH CO LTD

Human placenta chorionic mesenchymal stem cell separation method

The invention discloses a human placenta chorionic mesenchymal stem cell separation method, which comprises: 1) taking healthy human placenta chorionic tissue, cutting into strip blocks, and completely rinsing with physiological saline; 2) adding a proper amount of physiological saline, chopping the chorion into fine particles by using a homogenizer, and rinsing with physiological saline until the solution is clarified; 3) treating again with the homogenizer to achieve micro-particles, and carrying out centrifugation to remove the upper layer blood cells; 4) adding an enzyme to the precipitate, digesting, carrying out centrifugation, and separating the precipitate; 5) carrying out centrifugal washing with physiological saline twice, adding a culture medium to the precipitate, inoculating, and culturing; and 6) discarding the tissue blocks and the culture medium at the 7th day, rinsing the bottom of the culture flask by using physiological saline, adding a fresh culture medium, changing the culture medium every 3 days until the cell fusion degree achieves 80-90%, and carrying out digestion passage with trypsin to obtain the human placenta chorionic mesenchymal stem cells. According to the present invention, the operation is simple and rapid, and the high-quality human placenta chorionic mesenchymal stem cells can be obtained.
Owner:GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER

Cluster layout of completely automatic biological product system

The invention relates to the field of culture containers, especially to a cluster layout of a completely automatic biological product system. The cluster layout of the completely automatic biologicalproduct system comprises a culture container bottle whole body, a fully permeable cap and cell adherent wallboards, wherein the culture container bottle whole body comprises a bottom collector of centrifugal bottles, a bottle mouth, an inner cell adherent wall, a slope board and the cell adherent wallboards, the slope board is arranged in the bottom collector of centrifugal bottles, the inner celladherent wall is arranged at one end of the bottom collector of centrifugal bottles, multiple groups of cell adherent wallboards are arranged in the inner cell adherent wall, and a graduation scaleplate is arranged outside the culture container bottle whole body. According to the present invention, the cluster layout of the complete automatic biological product system can be used as a single container in the process of adherent cell culture, can be used for culture, centrifugation, sample observation, transportation and other operations, the operation of opening/closing the cap, the operationof liquid transporting, storage of multi-type containers and conversion of multi-type containers in the whole process of automated production can be simplified, and the production efficiency and benefit can be effectively improved.
Owner:INNOVEL INTELLIGENT TECH CO LTD

Laboratory organic waste liquid on-site real-time treatment system

The invention provides a laboratory organic waste liquid on-site real-time treatment system. A waste liquid reaction device (1) comprises a first-stage reaction body (11) and a second-stage reaction body (12) communicated through a channel; the first-stage reaction body (11) is olive-shaped, is internally provided with a flame holder (8) and is filled with primary air and waste liquid; the second-stage reaction body (12) is in a straight eccentric necking shape, the left side of the top is an inclined plane, the right side is a vertical plane, and the section of a top outlet is about 1 / 2-2 / 3 of the section of the middle section; and reactants of the waste liquid reaction device (1) are cooled by a waste heat recovery device (3), quenched and desulfurized by a flue gas quenching and desulfurizing integrated device (4), dewatered by a dewatering device (5) and finally filtered by a flue gas purifying device (6) to remove harmful components. The first-stage reaction body can guarantee a waste liquid reaction space; the second-stage reaction body can guarantee the waste liquid treatment temperature; and the laboratory organic waste liquid on-site real-time treatment system is beneficial to staged treatment of the waste liquid, increases the reactant reaction flow, and ensures the reaction temperature, space and time of the waste liquid.
Owner:TSINGHUA UNIV

A real-time treatment system for laboratory organic waste liquid

The invention provides an on-site real-time treatment system for laboratory organic waste liquid, wherein the waste liquid reaction device (1) includes a primary reactant (11) and a secondary reactant (12) communicated through channels; the primary reactant ( 11) It is olive-shaped, with a flame stabilizer (8) inside and primary air and waste liquid; the secondary reaction body (12) is in the shape of a straight cylinder with eccentric necking, the left side of the top is a slope and the right side is a vertical surface, and the top The outlet section is about 1 / 2-2 / 3 of the middle section; the reactant of the waste liquid reaction device (1) is cooled by the waste heat recovery device (3), quenched and desulfurized by the flue gas quenching and desulfurization integrated device (4), and dehydrated The device (5) removes moisture, and finally the harmful components are filtered out by the flue gas purification device (6). The first-stage reaction body can guarantee the reaction space of the waste liquid; the second-stage reaction body can ensure the waste liquid treatment temperature; the invention is beneficial to the staged treatment of the waste liquid, increases the reaction process of the reactants, and ensures the reaction temperature, space and time of the waste liquid.
Owner:TSINGHUA UNIV

Coil type heat exchanger

The invention provides a coil type heat exchanger. The coil type heat exchanger comprises a coil and a metal corrugated pipe. The coil type heat exchanger is characterized in that the metal corrugated pipe with the large caliber is mounted in the coil, openings in the two ends of the metal corrugated pipe are exposed out of openings in the two ends of the coil, and the openings in the two ends of the coil and the outer wall of the metal corrugated pipe are sealed through injection molding, hot melting, welding or colloid; a first joint is mounted at one end of the coil, a second joint is mounted at the other end of the coil, a third joint is the opening in one end of the metal corrugated pipe, and a fourth joint is the opening in the other end of the metal corrugated pipe; the metal corrugated pipe is a copper corrugated pipe or a stainless steel corrugated pipe; and the coil is a rubber coil or a plastic coil. According to the coil type heat exchanger, the runner is long, the metal corrugated pipe with the large caliber is used for flowing of waste hot water, blocking is not likely to happen, the heat exchange efficiency is high, and the coil type heat exchanger can be widely applied to recovery of waste hot water and hot energy generated in household bathing, hairdressing and beauty, large bath centers and industrial production.
Owner:刘洋豪

A method for isolating human placental chorionic mesenchymal stem cells

The invention discloses a human placenta chorionic mesenchymal stem cell separation method, which comprises: 1) taking healthy human placenta chorionic tissue, cutting into strip blocks, and completely rinsing with physiological saline; 2) adding a proper amount of physiological saline, chopping the chorion into fine particles by using a homogenizer, and rinsing with physiological saline until the solution is clarified; 3) treating again with the homogenizer to achieve micro-particles, and carrying out centrifugation to remove the upper layer blood cells; 4) adding an enzyme to the precipitate, digesting, carrying out centrifugation, and separating the precipitate; 5) carrying out centrifugal washing with physiological saline twice, adding a culture medium to the precipitate, inoculating, and culturing; and 6) discarding the tissue blocks and the culture medium at the 7th day, rinsing the bottom of the culture flask by using physiological saline, adding a fresh culture medium, changing the culture medium every 3 days until the cell fusion degree achieves 80-90%, and carrying out digestion passage with trypsin to obtain the human placenta chorionic mesenchymal stem cells. According to the present invention, the operation is simple and rapid, and the high-quality human placenta chorionic mesenchymal stem cells can be obtained.
Owner:GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER

Annular casing pipe type heat exchanger

The invention provides an annular casing pipe type heat exchanger. The annular casing pipe type heat exchanger comprises a plastic pipe, a stainless steel corrugated pipe and bundling pieces. The annular casing pipe type heat exchanger is characterized in that the stainless steel corrugated pipe is arranged in the plastic pipe, openings in the two ends of the stainless steel corrugated pipe are exposed out of openings in the two ends of the plastic pipe, and the openings in the two ends of the plastic pipe and the outer wall of the stainless steel corrugated pipe are sealed through injection molding or hot melting or welding or glue; a hole is formed in the pipe wall at one end of the plastic pipe, and a first connector is arranged on the hole in the pipe wall in the manner of injection molding or hot melting or welding or glue; a hole is formed in the pipe wall at the other end of the plastic pipe, a second connector is arranged on the hole of the pipe wall, the opening in one end of the stainless steel corrugated pipe is provided with a third connector, and the opening in the other end of the stainless steel corrugated pipe is provided with a fourth connector; and the plastic pipe provided with the stainless steel corrugated pipe is manufactured into a spiral shape, and the plastic pipe is twisted by the bundling pieces to be shaped into a ring shape. Waste hot water flows through the stainless steel corrugated pipe with the diameter ranging from 10 mm to 70 mm, blockage is not likely to happen, and the heat energy of the waste hot water can be better recycled.
Owner:刘洋豪
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