Method for mass production of pseudorabies virus vaccine

A pseudorabies virus, large-scale technology, applied in biochemical equipment and methods, tissue cell/virus culture devices, antiviral agents, etc., can solve the monitoring and control of environmental conditions, high labor intensity, low cell density, etc. problems, to achieve uniform and stable product quality, reduce the probability of contamination, and increase the effect of virus content
CN101804203AInactive Publication Date: 2010-08-18PU LIKE BIO ENG
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
PU LIKE BIO ENG
Publication Date
2010-08-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for mass production of pseudorabies virus vaccine, comprising the following steps: (a) adding netty polyester fiber which serves as a carrier into a bioreactor provided with a tide type micro-carrier suspension culture system and inoculating cells for producing vaccine; (b) inoculating pseudorabies virus vaccine when the culture cell grows to a certain intensity, so that the cells are infected by the pseudorabies virus vaccine; (c) reproducing the virus in great numbers under appropriate conditions; (d) harvesting the virus when cytopathic rate reaches above 70%; (e) carrying out freeze thawing on the harvested virus for once or twice to lead the cells to completely come off and disperse and then adding freeze-drying protective agent, evenly mixing the mixture, packaging the mixture in fixed volume and freeze-drying.The method of the invention has the advantages of good stability, explicit process control indicators, good controllability, easy operation, large process scale and the like.
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Description

technical field

[0001] The invention belongs to the technical field of biological products, and relates to a large-scale production method of pseudorabies virus vaccine. Background technique

[0002] Pseudorabies virus (PRV) belongs to the alphaherpesvirus subfamily of the family Herpesviridae, and has the characteristics of rapid growth on cell growth, strong neurotropism and latent infection. The virus can infect pigs, cattle, sheep, dogs, cats, rabbits, mice, minks, foxes and other livestock and wild animals under natural conditions. Pigs are the reservoirs and transmitters of the virus, mainly manifested as abortion, stillbirth or mummified fetuses in pregnant sows, neurological symptoms, paralysis, failure and even death in newborn piglets, with a mortality rate of almost 100%, and the incidence of weaned piglets is 20-40%. %, and the mortality rate is 10-20%. The disease has become the main cause of reproductive dysfunction syndrome in breeding pigs and is increasing ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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