6results about How to "Uniform state" patented technology

The utility model discloses a craft beer size mixing tank which comprises a base assembly, the top of the base assembly is provided with a size mixing tank, the outer wall of the size mixing tank is fixedly connected with a mixing assembly, and the bottom of the base assembly is fixedly connected with an air cooler. The mixing assembly comprises a connecting frame mechanism, the outer wall of the connecting frame mechanism is fixedly connected with a telescopic hydraulic cylinder, one end of the telescopic hydraulic cylinder is fixedly connected with a sealing cover, and one end of the telescopic hydraulic cylinder is fixedly connected with a stirring mechanism; the connecting frame mechanism comprises a connecting cylinder fixedly arranged on the size mixing tank, and the outer wall of the connecting cylinder is fixedly connected with a direction adjusting motor; according to the craft beer pulp mixing tank, through the arrangement of the mixing assembly, in the using process, a direction adjusting motor is started and controlled to be used, so that a sealing cover or a stirring mechanism is selected to be used according to needs, a telescopic hydraulic cylinder is started and controlled to be used for stuffing the sealing cover or the stirring mechanism into a stirring hole for filling, and then the sealing effect on the tank body is achieved.

This invention provides a gravity-based multi-tension-leg floating wind turbine platform, comprising: a wind turbine and its tower, a buoyancy chamber, a tensioning mooring system, and a gravity foundation. The wind turbine and its tower are mounted on top of the buoyancy chamber; the buoyancy chamber is connected to the gravity foundation arranged on the seabed via the tensioning mooring system to form a tension leg restraint system. The tensioning mooring system, by significantly increasing the number and rationally distributing the cables, distributes the platform's tension under the influence of wind, waves, and currents among more tensioning components, thereby reducing the tensile force and fatigue load requirements of a single tensioning mooring member and its end connecting components, and reducing the engineering implementation difficulty caused by excessive stress on the tension legs. The gravity foundation is fixed to the seabed by its own weight, eliminating the need for piling operations required for traditional tension-leg platforms, significantly reducing the difficulty and cost of offshore construction.

The invention provides a non-coding RNA combination and application thereof in mouse totipotent stem cell induction, and relates to the technical field of biology. The invention provides a non-coding RNA combination for inducing totipotency of mouse embryonic stem cells. The non-coding RNA combination comprises an enhancer RNA with a sequence as shown in SEQ ID NO: 1, an enhancer RNA with a sequence as shown in SEQ ID NO: 2 and a long-chain non-coding RNA with a sequence as shown in SEQ ID NO: 3. According to the invention, the CRISPR inhibition system is used for synergistically inhibiting the expression of the specific endogenous non-coding RNA combination for the first time, and the mouse embryonic stem cells can be efficiently promoted to be converted into 2C-like cells. Exogenous transcription factor integration is not needed, so that the safety risk of a genome is avoided; precise targeting is realized through sgRNA, and the defect of non-specific off-target of chemical small molecules is overcome; a remarkable synergistic effect is generated, and the induction efficiency of 2C-like cells can be remarkably improved to about 9.09%. The invention provides a safer, more efficient and controllable new strategy for stem cell fate reprogramming and early development research.

The application discloses a tissue culture method using maple zygotic embryos as explants and relates to the technical field of plant tissue culture.The method comprises the following steps: S1, cutting the zygotic embryos of immature maple fruits and inoculating the zygotic embryos onto a callus induction culture medium to induce callus; S2, transferring the callus induced in S1 to a bud induction culture medium to induce adventitious buds; and S3, inoculating the adventitious buds formed in step S2 into a strong seedling rooting culture medium to induce rooting, directly forming regenerated seedlings and carrying out transplanting culture.Compared with the culture methods using other maple tissues as explants, the method is more convenient, has higher genotype universality and is more efficient in propagation.

The present invention relates to a method for producing whey protein, the method comprising a hydrolysis step and an enzyme inactivation step, wherein in the hydrolysis step, a whey protein aqueous solution is hydrolyzed in the presence of an enzyme, the hydrolysis temperature is 55°C or lower, the hydrolysis time is 60 minutes or less, and the whey protein aqueous solution is subjected to heat history at a temperature of not more than 40°C before the hydrolysis, and before the end of the hydrolysis, lactose is added to a system containing a whey protein hydrolysis component, the amount of the lactose added being 15% to 300% by mass of the whey protein content; and in the enzyme inactivation step, after the end of the hydrolysis, the hydrolyzate solution is heated to inactivate the enzyme after adjusting the pH to 7.2 to 8.0.