Preparation method of electrochemical aptamer sensor for detecting aflatoxin B1

An aptamer sensor, aflatoxin technology, applied in the direction of electrochemical variables of materials, etc., to achieve the effects of good reproducibility, simple construction process and low detection limit

Active Publication Date: 2019-06-11
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no method for combining aptamers with sensors for the detection of aflatoxin B1

Method used

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  • Preparation method of electrochemical aptamer sensor for detecting aflatoxin B1

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Embodiment 1

[0039] A preparation method for detecting aflatoxin B1 electrochemical aptamer sensor, the sensor includes a working electrode, a reference electrode and a counter electrode, the working electrode is a glassy carbon electrode, and the reference electrode is saturated potassium chloride glycerin Mercury electrode, counter electrode is platinum column electrode, comprises the following steps:

[0040] 1) Modify mesoporous carbon on the working electrode: Dissolve an appropriate amount of mesoporous carbon in N, N-dimethylformamide aqueous solution, obtain a uniformly dispersed mesoporous carbon suspension by ultrasonication, and modify the mesoporous carbon on the surface of the working electrode to Increase the surface area of ​​the working electrode;

[0041] 2) Modification of gold nanoparticles: immerse the modified working electrode in step 1) into HAuCl 4 and H 2 SO 4 Constant potential deposition in the mixed solution to change the surface properties of the working ele...

Embodiment 2

[0046] A preparation method for detecting aflatoxin B1 electrochemical aptamer sensor, the sensor includes a working electrode, a reference electrode and a counter electrode, the working electrode is a glassy carbon electrode, and the reference electrode is saturated potassium chloride glycerin Mercury electrode, counter electrode is platinum column electrode, comprises the following steps:

[0047] 1) Modifying the working electrode with mesoporous carbon: modifying the surface of the working electrode with mesoporous carbon to increase the surface area of ​​the working electrode; the specific steps are:

[0048] Take 1mg of mesoporous carbon and dissolve it in 2ml of N,N-dimethylformamide aqueous solution (v:v, 1:1), sonicate for 30min to obtain a uniformly dispersed concentration of 0.5mg mL -1 Take the mesoporous carbon suspension, take 2-14 μL of the mesoporous carbon suspension and drop it on the pretreated clean working electrode, and let it dry naturally to obtain the ...

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Abstract

The invention provides a preparation method of an electrochemical aptamer sensor for detecting aflatoxin B1. The preparation method comprises the following steps of (1) dropwise adding mesoporous carbon onto a work electrode; (2) performing potentiostatic gold nanoparticle deposition for modifying CMK/GCE; (3) dropwise adding an AFB1 aptamer modified with sulfydryl at one end onto an AuNPs/CMK/GCEelectrode; (4) immersing the Apt/AuNPs/CMK/GCE into an MB (Methylene Blue) solution, so that the MB is adsorbed on an aptamer chain to obtain an MB-Apt/AuNPs/CMK/GCE electrode; and (5) detecting thereduction peak current value. The electrochemical aptamer sensor for detecting aflatoxin B1 can achieve the goal of quantitatively detecting a target object; the characteristics of high speed and operation simplicity are realized; and the defects of operation complexity and long detection period of a large-scale detecting instrument are overcome.

Description

technical field [0001] The invention belongs to the field of photoelectric materials and photoelectrochemical detection, and in particular relates to a preparation method of an electrochemical aptamer sensor for detecting aflatoxin B1. Background technique [0002] Aflatoxin (Aflatoxin) is a carcinogenic mycotoxin. Aflatoxin has different specific structures and toxicity differences. Among them, AFB1 is the most toxic and carcinogenic, and has been identified as a class I carcinogen by the International Organization for Research on Cancer. When it enters the animal body, it will show strong pro-hepatotropism, causing hepatic hemorrhage, fatty degeneration, bile duct hyperplasia and liver cancer. In addition, it will also cause inflammatory lesions in the front part of the stomach, changes in hematopoietic function and blood components, and immune function. damage to other systems. [0003] Electrochemical sensor is a small and portable analysis device, which is composed of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30
Inventor 刘冰赵耀帅吴秋月潘明飞王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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