2results about How to "Boost electrical signal" patented technology

The application discloses a preparation method of a dual-mode CD122 gene thalassemia detection self-powered biosensor, successfully applies an enzyme biological fuel cell (EBFC) to dual-mode detection of CD122 gene thalassemia, realizes quantitative analysis through changes of EBFC output voltage and changes of electrolyte color (RGB Blue value), and mainly concentrates on a cathode of the EBFC in the self-powered design. When CD122 exists, an electrode starts catalytic hairpin self-assembly (CHA) amplification, realizes recycling of CD122, and provides a binding site for subsequent hybridization chain reaction (HCR) amplification, at this time, methylene blue (MB) adsorbed by a biological cathode increases, and an electric signal is enhanced, and meanwhile, electrolyte color becomes lighter (RGB Blue value becomes larger). The self-powered CD122 dual-mode biosensor based on the EBFC disclosed by the application can realize simple, rapid, sensitive and efficient detection of a target.

The invention belongs to the technical field of biomarker detection, and particularly relates to a suspended graphene field effect transistor, a preparation method thereof and application of the suspended graphene field effect transistor in a biosensor. The suspended graphene field effect transistor provided by the invention comprises a substrate, wherein a groove is formed in the upper surface of the substrate; the insulating layers are arranged on the upper surface, except for the groove, of the substrate, the side wall of the groove and the bottom surface of the groove, the electrode layers are arranged on the upper surfaces of the insulating layers and comprise source electrodes and drain electrodes, and the source electrodes and the drain electrodes are located on the upper surfaces of the insulating layers on the two sides of the groove respectively; and the single-layer graphene film is arranged on the upper surface of the electrode layer, covers the groove and is communicated with the source electrode and the drain electrode. The suspended graphene field effect transistor provided by the invention can realize miR-21 detection without marking, is high in detection sensitivity and low in detection limit, can realize miR-21 detection in a fly mole magnitude, and provides a technical approach for promoting early cancer diagnosis.