Preparation method and application of sensor based on screen-printed carbon electrode

A technology of screen printing and carbon electrodes, which is applied in the field of biosensors, can solve the problems of low residue and quantitative detection, inaccurate detection results, and the influence of spectrophotometry, etc., and can be mass-produced at cost, with high stability and sensitivity , design flexible effects

Active Publication Date: 2015-09-23
SHANDONG EMBER INSTR
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

The principle of spectrophotometry is based on the change of absorbance for detection, but a large number of pigments in vegetables and fruits will have a great impact on spectrophotometry, resulting in inaccurate detection results
M

Method used

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  • Preparation method and application of sensor based on screen-printed carbon electrode
  • Preparation method and application of sensor based on screen-printed carbon electrode
  • Preparation method and application of sensor based on screen-printed carbon electrode

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Embodiment 1: A kind of sensor preparation method based on screen printing carbon electrode

[0056] (1) Cleaning of the screen-printed carbon electrode before the preparation of the amperometric acetylcholinesterase sensor, place the screen-printed carbon electrode in 1mM NaOH solution for 5 min, rinse with ultrapure water, and N 2 blow dry. Afterwards, the electrode was placed in 1mM HCl solution for 5min sonication, rinsed with ultrapure water, N 2 blow dry. Finally, rinse the electrode with ethanol, N 2 blow dry;

[0057] (2) Activation of the electrode before the preparation of the amperometric acetylcholinesterase sensor, scanning the current-time curve for 300s in pH 5.0 phosphate buffer, and then scanning the cyclic voltammetry curve until the performance is stable;

[0058] (3) Preparation of dicyclohexyl phthalate / multi-walled carbon nanotubes, adding 0.1g chitosan to 50mL concentration of 1.0% acetic acid solution to form a 0.2% chitosan solution, magneti...

Embodiment 2

[0060] Example 2: Using the prepared amperometric acetylcholinesterase sensor to detect vegetable samples

[0061] (1) The acetylcholinesterase sensor prepared according to Example 1 was subjected to a cyclic voltammetry test at a scan rate of 50 mV / s in a phosphate buffer solution of pH 7.5 containing 1.0 mM thioacetylcholine chloride (ATCl), The potential window is 0V~1.0V;

[0062] (2) Prepare chlorpyrifos standard solution. When measuring pesticides, immerse the above-mentioned acetylcholinesterase sensor in different concentrations of pesticide standard solutions for 10 minutes, and then add 15~20mL of chlorpyrifos containing 1.0mM thioacetylcholine chloride (ATCl) to the reaction pool. Phosphate buffered saline, carry out cyclic voltammetry scan, and inhibition rate I can be obtained by following formula:

[0063] I(%)=(I P, control ?I P, exp ) / I P, control ×100%

[0064] where I P, control and I P, exp Respectively, the peak current of the modified electrode in t...

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Abstract

The invention discloses a preparation method and an application of a sensor based on a screen-printed carbon electrode and belongs to the technical field of biological sensors. The method comprises steps as follows: a dicyclohexyl phthalate/ multiwalled carbon nanotube nano material is prepared and dripped on the surface of the screen-printed carbon electrode layer by layer, then acetylcholine esterase is dripped on the screen-printed work electrode surface modified with a dicyclohexyl phthalate/multiwalled carbon nanotube, and the acetylcholine esterase biological sensor for detecting organophosphorus and carbamate pesticides is prepared. The prepared sensor has the characteristics of simple work procedure, convenience in operation, low cost, high detection sensitivity, strong electric signal and simplicity in electrode pre-treatment and is applied to detection of pesticide residues, and the detection limit reaches 0.05 mu g/L.

Description

technical field [0001] The invention relates to a preparation method and application of a sensor based on a screen-printed carbon electrode, and belongs to the technical field of biosensors. Background technique [0002] Pesticides are important means of agricultural production, and play a very important role in the prevention and control of diseases, insects, weeds, and rodents in the production of agricultural products. However, pesticide pollution and its harmful consequences are serious, and the losses caused by pesticides to environmental pollution are various, including pollution to water environment, pollution to soil, pollution to air, impact on environmental organisms and hazards to human health, etc. At present, due to the general lack of scientific knowledge of pesticide use by users and one-sided pursuit of yield, the agricultural products currently on the market have pesticide residues to varying degrees, and their harm has increasingly attracted public attenti...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 孙霞陈栋菲徐建光郭业民王相友
Owner SHANDONG EMBER INSTR
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