Silk-screen printing electrode sensor preparation method based on ionic liquid-multi-wall carbon nano-tube modification

A technology of multi-walled carbon nanotubes and screen-printed electrodes, applied in the field of biosensors, can solve the problems of low residue and quantitative detection, the influence of spectrophotometry, and inaccurate detection results, and achieve excellent functionality and compatibility High performance, high stability and sensitivity, high chemical stability and thermal stability

Inactive Publication Date: 2015-11-04
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The principle of spectrophotometry is based on the change of absorbance for detection, but a large number of pigments in vegetables and fruits will have a great impact on spectrophotometry, resulting in inaccurate detection results
Moreover, the above method has disadvantages such as low recovery rate, high proportion of false detection and missed detection, poor repeatability, and difficulty in meeting the requirements of low residue and quantitative detection.

Method used

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  • Silk-screen printing electrode sensor preparation method based on ionic liquid-multi-wall carbon nano-tube modification
  • Silk-screen printing electrode sensor preparation method based on ionic liquid-multi-wall carbon nano-tube modification
  • Silk-screen printing electrode sensor preparation method based on ionic liquid-multi-wall carbon nano-tube modification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: A method for preparing a sensor based on screen-printed carbon electrodes modified by ionic liquid-multi-walled carbon nanotubes

[0053] (1) Cleaning of the screen-printed carbon electrode before the preparation of the amperometric acetylcholinesterase sensor. Place the screen-printed carbon electrode in 1mM NaOH solution for 5min, rinse with ultrapure water, N 2 blow dry. Afterwards, the electrode was placed in 1mM HCl solution for 5min sonication, rinsed with ultrapure water, N 2 blow dry. Finally, rinse the electrode with ethanol, N 2 Blow dry; (2) Activation of the electrode before the preparation of the amperometric acetylcholinesterase sensor. Scan the current-time curve for 300s in pH5.0 phosphate buffer, and then scan the cyclic voltammetry curve until the performance is stable; (3) Preparation of ionic liquid / multi-walled carbon nanotubes. Disperse 4mg of N-octylpyridinium hexafluorophosphate (ionic liquid) in 4mL of absolute ethanol and disper...

Embodiment 2

[0054] Example 2: Using the prepared amperometric acetylcholinesterase sensor to detect vegetable samples

[0055] (1) The acetylcholinesterase sensor prepared according to Example 1 was subjected to a cyclic voltammetry test at a scan rate of 50 mV / s in a phosphate buffer solution of pH 7.5 containing 1.0 mM thioacetylcholine chloride (ATCl), The potential window is 0V~1.0V; (2) Prepare chlorpyrifos standard solution. When measuring pesticides, immerse the above-mentioned acetylcholinesterase sensor in different concentrations of pesticide standard solutions for 10 minutes, and then add 15~20mL containing 1.0mM chlorine to the reaction pool. Thioacetylcholine (ATCl) in phosphate buffered saline solution, carry out cyclic voltammetry scanning, and the inhibition rate I can be obtained by the following formula:

[0056] I(%)=(I P, control ?I P, exp ) / I P, control ×100%

[0057] where I P, control and I P, exp Respectively, the peak current of the modified electrode in the...

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Abstract

The present invention discloses a silk-screen printing electrode sensor preparation method based on ionic liquid-multi-wall carbon nano-tube modification, and belongs to the technical field of biological sensors. According to the method, an ionic liquid-multi-wall carbon nano-tube nanometer material is prepared and then coats the surface of a silk-screen printing carbon electrode in a dropwise manner, and then acetylcholine esterase coats the surface of the ionic liquid-multi-wall carbon nano-tube nanometer material modified silk-screen printing work electrode in a dropwise manner so as to prepare the acetylcholine esterase biological sensor for detecting organic phosphorus and carbamate pesticides. According to the present invention, the prepared sensor has characteristics of simple process, easy operation, low cost, high detection sensitivity, strong electrical signal and simple electrode pretreatment, and is suitable for detection of pesticide residues, wherein the detection limit is 0.05 [mu]g/L.

Description

technical field [0001] The invention relates to a sensor preparation method based on an ionic liquid-multi-walled carbon nanotube modified screen printing electrode, belonging to the technical field of biosensors. Background technique [0002] Pesticides are important means of agricultural production, and play a very important role in the prevention and control of diseases, insects, weeds, and rodents in the production of agricultural products. However, pesticide pollution and its harmful consequences are serious, and the losses caused by pesticides to environmental pollution are various, including pollution to water environment, pollution to soil, pollution to air, impact on environmental organisms and hazards to human health, etc. At present, due to the general lack of scientific knowledge of pesticide use by users and one-sided pursuit of yield, the agricultural products currently on the market have pesticide residues to varying degrees, and their harm has increasingly a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327B82Y30/00B82Y40/00
Inventor 孙霞陈栋菲徐建光郭业民王相友
Owner SHANDONG UNIV OF TECH
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