Preparation method and application of immunosensor constructed by difunctional marker

An immunosensor and marker technology, which can be used in analytical materials, biological tests, instruments, etc., to achieve the effect of increasing the current response

Inactive Publication Date: 2015-05-06
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing research results have shown that titanium dioxide doped with metal ions has a good effect in photocatalytic degradation of environmental pollutants, but it has not yet appeared in the preparation of sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. A method for preparing a bifunctional marker-secondary antibody hatch, comprising the following steps:

[0043] (1) Preparation of titanium dioxide nanomaterials

[0044] Add 2 mL of butyl titanate to 40 mL of ethylene glycol, stir at room temperature for 6 h to form a mixture, add this mixture to 150 mL of acetone, add 2 mL of water, stir vigorously for 1 h, centrifuge, wash with ethanol for 2 Once, dry at 50°C to obtain the titanium dioxide precursor; disperse 0.1 g of the titanium dioxide precursor into 20 mL of water, heat and reflux for 1 h under stirring; then centrifuge, wash with ethanol twice, and dry at 50°C to obtain the titanium dioxide nanometer material, spare;

[0045] (2) Copper doped titanium dioxide CuTiO 2 preparation of

[0046] Add 1 g of titania nanomaterials to 40 mL, 10 mol L -1 in NaOH solution, stirred for 2 h, put into a polytetrafluoroethylene reaction kettle, heated at 120°C for 18 h, took it out, cooled to room temperature, and used ...

Embodiment 2

[0052] 1. A method for preparing a bifunctional marker-secondary antibody hatch, is characterized in that, comprises the following steps:

[0053] (1) Preparation of titanium dioxide nanomaterials

[0054] Add 2 mL of butyl titanate to 50 mL of ethylene glycol, stir at room temperature for 8 h to form a mixture, add the mixture to 170 mL of acetone, add 3 mL of water, stir vigorously for 2 h, centrifuge, and wash with ethanol 4 times , dried at 50°C to obtain a titanium dioxide precursor; disperse 0.1 g of the titanium dioxide precursor into 20 mL of water, heat and reflux for 2 h under stirring conditions; then centrifuge, wash with ethanol four times, and dry at 50°C to obtain titanium dioxide nanomaterials ,spare;

[0055] (2) Copper doped titanium dioxide CuTiO 2 preparation of

[0056] Add 1 g of titania nanomaterials to 40 mL, 10 mol L -1 in NaOH solution, stirred for 3 h, put into a polytetrafluoroethylene reaction kettle, heated at 140°C for 24 h, took it out, coo...

Embodiment 3

[0062] 1. A method for preparing a bifunctional marker-secondary antibody hatch, comprising the following steps:

[0063] (1) Preparation of titanium dioxide nanomaterials

[0064] Add 2 mL of butyl titanate to 45 mL of ethylene glycol, stir at room temperature for 7 h to form a mixture, add the mixture to 160 mL of acetone, add 2.5 mL of water, stir vigorously for 1.5 h, centrifuge, wash with ethanol three times, Dry at 50°C to prepare the titanium dioxide precursor; disperse 0.1 g of the titanium dioxide precursor into 20 mL of water, heat and reflux for 1.5 h under stirring conditions; then centrifuge, wash with ethanol three times, and dry at 50°C to prepare titanium dioxide nanomaterials. spare;

[0065] (2) Copper doped titanium dioxide CuTiO 2 preparation of

[0066] Add 1 g of titania nanomaterials to 40 mL, 10 mol L -1 in NaOH solution, stirred for 2.5 h, put it into a polytetrafluoroethylene reaction kettle, heated at 130°C for 20 h, took it out, cooled to room t...

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PUM

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Abstract

The invention discloses a preparation method and application of an immunosensor constructed by a difunctional marker, belonging to the technical field of novel nano functional materials and biosensors. The preparation method is characterized by comprising the following steps: (1) preparing copper-doped titanium dioxide Cu@TiO2; (2) preparing the difunctional marker-secondary antibody incubation material Cu@TiO2-Ab2; and (3) preparing the immunosensor constructed by the difunctional marker and performing analytic application. The immunosensor constructed by the difunctional marker has the advantages of quickness in identification, high sensitivity, low detection limit, low cost, convenience in operation and capability of realizing high-sensitive, specific, quick and accurate detection of various tumor markers.

Description

technical field [0001] The invention belongs to the technical fields of functionalized nanomaterials, immune analysis and biosensing, and in particular relates to a preparation method and application of an immunosensor constructed with dual-functional markers. Background technique [0002] Since the 1980s, the research and development of electrochemical sensors has shown rapid progress. In recent years, with the rapid development of nanomaterial science and microelectronics technology, the cross integration of nanotechnology, life science, biotechnology, electroanalysis technology and surface science, the wide adoption of various new principles, new materials and new processes , electrochemical sensors continue to emerge and enter practical applications. [0003] Immunosensor is a biosensor that combines immunological methods and analytical chemistry. Through the specific combination of antigen and antibody, it has the advantages of high sensitivity, good selectivity, rapid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N27/30G01N27/48
CPCG01N33/532G01N33/54393
Inventor 魏琴张森杜斌庞雪辉马洪敏吴丹
Owner UNIV OF JINAN
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